Unveiling the potential of novel recyclable deep eutectic solvent pretreatment: Effective separation of lignin from poplar hydrolyzed residue.

To effectively convert the fermentable sugars present in lignocellulosic biomass into biofuels and additional value-added products, it is crucial to remove lignin from the biomass. With the intention of expeditiously remove lignin from poplar wood and improve cellulose saccharification, an innovative ternary deep eutectic solvent (DES) benzyl triethyl ammonium chloride-ethylene glycol-FeCl3 (T-EG-F) was studied for the pretreatment of poplar hydrolyzed residue (PHR). The results revealed that following T-EG-F DES pretreatment at 130 °C for 4 h, the lignin removal rate reached 91.88 %. The effect of DES on PHR and regenerated lignin was comprehensively investigated using X-ray diffractometer (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), Thermogravimetric (TG) and other characterization methods, providing valuable insights into the mechanism of this innovative biomass pretreatment. Moreover, there was a significant improvement in the enzyme digestibility of the DES pretreatment residue. At 48 h, the enzyme load of 30 FPU/g cellulose achieved a remarkable enzyme digestibility of 97.31 %, and this value exhibited a notable increase of 6.56 times compared to the untreated poplar sample. In addition, the T-EG-F could be recycled and reused. This study demonstrates that the potential of T-EG-F DES pretreatment as a green and efficient method for lignin dissociation from lignocellulosic biomass, offering a promising approach for biomass component separation.

Evaluating the potential of different bioaugmented strains to enhance methane production during thermophilic anaerobic digestion of food waste.

Bioaugmentation technology for improving the performance of thermophilic anaerobic digestion (TAD) of food waste (FW) treatment is gaining more attention. In this study, four thermophilic strains (Ureibacillus suwonensis E11, Clostridium thermopalmarium HK1, Bacillus thermoamylovorans Y25 and Caldibacillus thermoamylovorans QK5) were inoculated in the TAD of FW system, and the biochemical methane potential (BMP) batch study was conducted to assess the potential of different bioaugmented strains to enhance methane production. The results showed that the cumulative methane production in groups inoculated with E11, HK1, Y25 and QK5 improved by 2.05%, 14.54%, 19.79% and 9.17%, respectively, compared with the control group with no inoculation. Moreover, microbial community composition analysis indicated that the relative abundance of the main hydrolytic bacteria and/or methanogenic archaea was increased after bioaugmentation, and the four strains successfully became representative bacterial biomarkers in each group. The four strains enhanced methane production by strengthening starch, sucrose, galactose, pyruvate and methane metabolism functions. Further, the correlation networks demonstrated that the representative bacterial genera had positive correlations with the differential metabolic functions in each bioaugmentation group. This study provides new insights into the TAD of FW with bioaugmented strains.

Enhancing biomass conversion: Efficient hemicellulose removal and cellulose saccharification in poplar with FeCl3 coupled with acidic electrolyzed water pretreatment.

Due to the recalcitrant structure of woody biomass such as poplar, the efficient disassembly and separation of hemicellulose component from woody biomass is crucial for green biomass processing and full component utilization. This study presented an environmentally friendly approach to utilize acidic electrolyzed water (AEW) combined with metal salts and investigated its pretreatment effects on hemicellulose removal and cellulose and lignin retention under different conditions. Meanwhile, the structural properties and enzymatic hydrolysis performance of the pretreated residues were also characterized. As a result, under the optimized pretreatment conditions (0.03 mol/L FeCl3 with AEW at 180 °C for 10 min), hemicellulose removal from poplar wood reached 98.64 %, accompanied by xylose recovery rate of 98.46 %, cellulose retention rate of 93.43 % and lignin retention rate of 94.29 %. Enzymatic hydrolysis rate of the pretreated cellulose-enriched substrate reached 97.65 %. Furthermore, comprehensive structural characterizations revealed that FeCl3 coupled with AEW pretreatment resulted in surface damage to the poplar wood, effective removal of the amorphous hemicellulose component, and partial destruction of the cellulose crystallinity. In conclusion, FeCl3 coupled with AEW pretreatment effectively separates hemicellulose, leading to significant alterations in biomass composition and structure, ultimately resulting in improved enzymatic digestion. These results provide theoretical support for targeted dissociation of hemicellulose and full component utilization of woody biomass.

Bacterial community compositions and nitrogen metabolism function in a cattle farm wastewater treatment plant revealed by Illumina high-throughput sequencing.

Bacteria play an important role in pollutant transformation in activated sludge-based wastewater treatment plants (WWTPs). Exploring the microbial community structure and diversity is essential to improving the performance of wastewater treatment processes. This study employed Illumina MiSeq high-throughput sequencing to investigate the microbial community composition and diversity in a cattle farm wastewater treatment plant (Cf-WWTP). The results showed that the dominant phyla in the whole process were Proteobacteria, Bacteroidetes, and Firmicutes. The principal coordinate analysis (PCoA) indicated that the different stages had a significant impact on the microbial community structure; Bacteroidetes was the dominant phylum in the anearobic stage and Proteobacteria was the dominant phylum in the anoxic-oxic stage. Redundancy analysis (RDA) revealed that total phosphorus (TP) was the most significant factor that regulated the microbial community composition, followed by chemical oxygen demand (COD), total nitrogen (TN), and pH. Proteobacteria, Patescibacteria, and Chloroflexi were simultaneously negatively correlated with TN, COD, and TP. Nitrogen metabolic pathway and transformation mechanism was elucidated by a complete denitrification function predicted with phylogenetic investigation of communities with reconstruction of unobserved states (PICRUSt), as well as detection of ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB). These results provide new insights into our understanding of microbial community and metabolic functions of Cf-WWTP.

Enhanced sludge reduction during swine wastewater treatment by the dominant sludge-degrading strains Chryseobacterium sp. B4 and Serratia sp. H1.

Sludge reduction is considered a main target for sludge treatment and an urgent issue for wastewater treatment. In this study, two dominant sludge-degrading strains, identified as Chryseobacterium sp. B4 and Serratia sp. H1, were used for inoculation in swine wastewater treatment to investigate the enhancement of sludge reduction. The results showed the volatile suspended solid (VSS) removal rate in experimental groups inoculated with Chryseobacterium sp. B4, Serratia sp. H1, and a combination of the two strains improved by 49.4%, 11.0%, and 30.5%, compared with the control with no inoculation. Furthermore, microbial community structure and functional prediction analyses indicated Proteobacteria, Firmicutes, Bacteroidetes and Actinobacteria could play an essential role in sludge reduction, and the dominant sludge-degrading strains B4 and H1 enhanced sludge reduction by strengthening carbohydrate, nucleotide, amino acid, and lipid metabolism and membrane transport functions. This study provides new insights into sludge reduction during wastewater treatment with dominant sludge-degrading strains.

MicroRNA expression, targeting, release dynamics and early-warning biomarkers in acute cardiotoxicity induced by triptolide in rats.

Tripterygium wilfordii Hook. F. is a plant used in traditional Chinese medicine to treat rheumatoid arthritis, lupus erythematosus, and psoriasis in China. However, its main active substance, triptolide, has toxic effects on the heart, liver, and kidneys, which limit its clinical application. Therefore, determining the mechanism of cardiotoxicity in triptolide and identifying effective early-warning biomarkers is beneficial for preventing irreversible myocardial injury. We observed changes in microRNAs and aryl hydrocarbon receptor (AhR) as potential biomarkers in triptolide-induced acute cardiotoxicity by using techniques such as polymerase chain reaction (PCR) assay. The results revealed that triptolide increased the heart/body ratio and caused myocardial fiber breakage, cardiomyocyte hypertrophy, increased cell gaps, and nuclear dissolution in treated male rats. Real-time PCR array detection revealed a more than 2-fold increase in the expression of 108 microRNA genes in the hearts of the male rats; this not only regulated the signaling pathways of ErbB, FOXO, AMPK, Hippo, HIF-1α, mTOR, and PI3K-Akt but also participated in biological processes such as cell adhesion, cell cycling, action potential, locomotory behavior, apoptosis, and DNA binding. Moreover, triptolide reduced the circulatory and cardiac levels of AhR protein as a target of these microRNAs and the messenger RNA expression of its downstream gene CYP1 A1. However, decreases in myocardial lactate dehydrogenase, creatine kinase MB, catalase, and glutathione peroxidase activity and an increase in circulating cardiac troponin I were observed only in male rats. Moreover, plasma microRNAs exhibited dynamic change. These results revealed that circulating microRNAs and AhR protein are potentially early-warning biomarkers for triptolide-induced cardiotoxicity.

[Study on different doses of mercury-containing preparations on acute toxicity in rabbits].

OBJECTIVE: To observe the effect of single administration of mercury- containing preparation Jiuyi Dan (calcined gypsum-Shengdan 9: 1) and Shengdan on acute toxicity of rabbits, in order to assess the safety of tested drugs. METHOD: The rabbits were randomly divided into 4 groups: the calcined gypsum group (excipient control), the Jiuyi Dan group, the 90 mg Shengdan group and the 180 mg Shengdan group. After 270 mg of calcined gypsum, 300 mg of Jiuyi Dan, 90 mg of Shengdan, and 180 mg of Shengdan were used on the surface of wounds (5 cm x 5 cm) on two sides of rabbit back for 5 h, the surfaces of wound were washed by water. The bloods were taken from the rabbit hearts before and after the drug administration for 24 h, 72 h, 7 d and 14 d for determining Hg level in blood and liver & kidney function indicators (ALT, AST, CREAT, and BUN). The rabbits were dissected after the drugs treatment for 14 d, and pathological tests were made for their livers and kidneys. RESULT: Compared with the calcined gypsum group, the 90 mg Shengdan group and the 180 mg Shengdan group showed significant increase (P < 0.01 or P < 0.05), as evidenced by increase in CREAT for 24 h and 72 h and increase in BUN for 24 h and on 7 d. AST is significantly increased as well (P < 0.01) for 24 h and 72 h compared to that of the group before drug treatment. The Hg level in blood was significantly enhanced (P < 0.01) after the rabbits were administrated with drugs for 24 h to 72 h. The pathological changes in livers and kidneys of rabbits were observed in the two doses of Shengdan treatment groups. CONCLUSION: The Hg blood levels were increased significantly in an obvious dose-effect relationship in all drugs treatment groups. Liver & kidney function indicators were influenced by Shengdan treatment to some extent. Meanwhile, pathological changes in rabbit livers and kidneys were also caused by Shengdan, while Jiuyi Dan has no significantly effect on livers and kidneys.

[Effects of external use of jiuyi dan for one month on blood and urine mercury levels and liver and kidney functions of rabbits].

OBJECTIVE: To observe the changes of the blood and urine mercury (Hg) levels and liver & kidney functions of rabbits after administration of Jiuyi Dan (calcined gypsum-Sheng Dan 9: 1) for 1 month and the recovery of rabbits after the drug withdrawal. METHOD: The rabbits were randomly divided into 2 groups: the calcined gypsum group and the Jiuyi Dan group. After 36 mg of calcined gypsum and 40 mg of Jiuyi Dan were used on the surface of wound (5 cm x 5 cm) on one side of rabbit back for 4 h, the surfaces of wound were washed by saline. The bloods were taken from the rabbit hearts before and after the drug administration for 14 and 28 days, and after the drug withdrawal for 7, 40, 71, and 92 days for determining Hg level in blood, and liver & kidney function indicators (ALT, AST, CREAT and BUN). The Hg level in urine collected from bladders was examined while rabbits were dissected after the drug withdrawal for 1, 40, 71, and 92 days. RESULT: The Hg level in blood was significantly increased (P < 0.01) after the rabbits were administrated with drugs for 14 and 28 days and after the drug treatment was stopped for 7 and 40 days. The Hg level in urine was significantly enhanced after the drug withdrawal for 1, 40, 71 days. However, the liver & kidney indicators were not influenced. CONCLUSION: The Hg level in rabbit blood and urine was significantly increased after the consecutive administration of double-dose Jiuyi Dan for 1 month. However, the blood Hg level and urine Hg level recover after the drug withdrawal for 71 days and 3 months, respectively. The liver & kidney indicators do not significantly change with the dose.

[Effect of Cordceps Sinensis on the expression of ICAM-1 and VCAM-1 in the kidney of spontaneously hypertensive rats].

OBJECTIVE: To observe the effect of Cordceps Sinensis (CS) on the expression of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) in the kidney of spontaneously hypertensive rats(SHR), and to investigate the mechanism of CS. METHODS: Male SHRs (23 week old) were randomly divided into 4 groups: a group without any treatment (Group S), a group treated with Cordceps sinensis at 4 g/(kg.d)(Group C), a group treated with fosinopril at 10 mg/(kg.d) (Group F), and a group received daily intragastric administration of CS at 4 g/(kg.d) and fosinopril at 10 mg/(kg.d) (Group CF). At the same time, 6 male WKY rats were used as normals controls. At the end of 8 weeks, all rats were sacrificed. Serum creatinine(Scr), 24 h urinary protein count, and the expression of ICAM-1 and VCAM-1 were examined by immunohistochemical technique and RT-PCR. RESULTS: Compared with the WKY rats, blood pressure, 24 h urinary protein count, Scr,and the expression of ICAM-1 andVCAM-1 in the kidney of SHR significantly increased (P<0.05).Compared with Group S, blood pressure decreased after treatment by fosinopril (P<0.05). Compared with Group S, the levels of Scr, 24 h urinary protein count, and glomerular lesion were significantly reduced in the CS and/or fosinopril treatment group. The expression of ICAM-1 and VCAM-1 was significantly decreased in these groups (P<0.05). CONCLUSION: CS may play a role in the protection and anti-fibrosis in the process of renal injury in SHR through reducing the expression of ICAM-1 and VCAM-1.