Prediction and verification of the prognostic biomarker SLC2A2 and its association with immune infiltration in gastric cancer.

Gastric cancer (GC) is the fifth most common cause of cancer-associated deaths; however, its treatment options are limited. Despite clinical improvements, chemotherapy resistance and metastasis are major challenges in improving the prognosis and quality of life of patients with GC. Therefore, effective prognostic biomarkers and targets associated with immunological interventions need to be identified. Solute carrier family 2 member 2 (SLC2A2) may serve a role in tumor development and invasion. The present study aimed to evaluate SLC2A2 as a prospective prognostic marker and chemotherapeutic target for GC. SLC2A2 expression in several types of cancer and GC was analyzed using online databases, and the effects of SLC2A2 expression on survival prognosis in GC were investigated. Clinicopathological parameters were examined to explore the association between SLC2A2 expression and overall survival (OS). Associations between SLC2A2 expression and immune infiltration, immune checkpoints and IC50 were estimated using quantification of the tumor immune contexture from human RNA-seq data, the Tumor Immune Estimation Resource 2.0 database and the Genomics of Drug Sensitivity in Cancer database. Differential SLC2A2 expression and the predictive value were validated using the Human Protein Atlas, Gene Expression Omnibus, immunohistochemistry and reverse transcription-quantitative PCR. SLC2A2 expression was downregulated in most types of tumor but upregulated in GC. Functional enrichment analysis revealed an association between SLC2A2 expression and lipid metabolism and the tumor immune microenvironment. According to Gene Ontology term functional enrichment analysis, SLC2A2-related differentially expressed genes were enriched predominantly in 'chylomicron assembly', 'plasma lipoprotein particle assembly', 'high-density lipoprotein particle', 'chylomicron', 'triglyceride-rich plasma lipoprotein particle', 'very-low-density lipoprotein particle'. 'intermembrane lipid transfer activity', 'lipoprotein particle receptor binding', 'cholesterol transporter activity' and 'intermembrane cholesterol transfer activity'. In addition, 'cholesterol metabolism', and 'fat digestion and absorption' were significantly enriched in the Kyoto Encyclopedia of Genes and Genomes pathway analysis. Patients with GC with high SLC2A2 expression had higher levels of neutrophil and M2 macrophage infiltration and a significant inverse correlation was observed between SLC2A2 expression and MYC targets, tumor mutation burden, microsatellite instability and immune checkpoints. Furthermore, patients with high SLC2A2 expression had worse prognosis, including OS, disease-specific survival and progression-free interval. Multivariate regression analysis demonstrated that SLC2A2 could independently prognosticate GC and the nomogram model showed favorable performance for survival prediction. SLC2A2 may be a prospective prognostic marker for GC. The prediction model may improve the prognosis of patients with GC in clinical practice, and SLC2A2 may serve as a novel therapeutic target to provide immunotherapy plans for GC.

Exfoliable and self-healable two-dimensional materials from wurtzite zinc chalcogenides as building blocks of nanodevices.

With the advent of graphene, two-dimensional (2D) materials have emerged as promising candidates for next-generation electronic and optoelectronic applications. The most anticipated 2D materials have been synthesized and exploited for novel applications. Multilayered zinc chalcogenides (ZnX) are the best precursors for obtaining atomic layer two-dimensional materials by exfoliation. Therefore, we carry out a detailed density functional theory-based study to achieve an exfoliation process of ZnX non-van der Waals sheets by straining and provide a microscopic understanding of the ferroelectric, optic, and spin behaviors of ZnX systems and the corresponding self-healable two-dimensional ZnX devices. The results revealed that 2D ZnX sheets can be obtained when strain is 14% for ZnS and ZnSe, and the peak values of exfoliation energy have a similar order of magnitude to those of traditional 2D materials, indicating the possibility of obtaining 2D ZnX monolayers. For intrinsic 2D ferroelectric materials with in-plane electric polarization, the direction of ZnX sheets can be reversed using an electric field with an energy barrier of ∼0.175 eV per atom for ZnSe, offering a promising functional basis for their application in ferroelectric nanodevices. The first absorption of photons for polarization perpendicular to the monolayer plane occurs in a high energy range of photons, facilitating their application in LEDs. The spin splitting in non-centrosymmetric ZnX crystals exhibits a Rashba spin-texture according to first-principles calculations. The self-healable two-dimensional nanodevices have a smooth curve from -0.5 to 0.5 eV. This work indicates the potential value of non-van der Waals ZnX 2D materials for their application in photoelectric and spintronic nanodevices.

Trajectory Tracking Coordinated Control of 4WID-4WIS Electric Vehicle Considering Energy Consumption Economy Based on Pose Sensors.

In order to improve the stability and economy of 4WID-4WIS (four-wheel independent drive-four-wheel independent steering) electric vehicles in trajectory tracking, this paper proposes a trajectory tracking coordinated control strategy considering energy consumption economy. First, a hierarchical chassis coordinated control architecture is designed, which includes target planning layer, and coordinated control layer. Then, the trajectory tracking control is decoupled based on the decentralized control structure. Expert PID and Model Predictive Control (MPC) are employed to realize longitudinal velocity tracking and lateral path tracking, respectively, which calculate generalized forces and moments. In addition, with the objective of optimal overall efficiency, the optimal torque distribution for each wheel is achieved using the Mutant Particle Swarm Optimization (MPSO) algorithm. Additionally, the modified Ackermann theory is used to distribute wheel angles. Finally, the control strategy is simulated and verified using Simulink. Comparing the control results of the average distribution strategy and the wheel load distribution strategy, it can be concluded that the proposed coordinated control not only provides good trajectory tracking but also greatly improves the overall efficiency of the motor operating points, which enhances the energy economy and realizes the multi-objective coordinated control of the chassis.

Systematic design method for generation, storage, and reconstruction of holographic images.

Metasurface has extraordinary capability in the wavefront manipulation of electromagnetic waves, which provides an effective method for meta-holographic technology. However, holographic technology mostly focuses on the generation of the single-plane image, which lacks a systematic design method to generate, store, and reconstruct multi-plane holographic images. In this paper, the Pancharatnam-Berry phase meta-atom is designed as electromagnetic controller with the characteristics of the full phase range and high reflection amplitude. Different from the single-plane holography method, a novel multi-plane retrieval algorithm is proposed for the computation of the phase distribution. The metasurface, containing only 24 × 24 (30 × 30) elements, can produce high-quality single-(double-) plane image(s) with fewer elements. Meanwhile, the implementation of the compressed sensing approach stores almost all the holographic image information under a 25% compression ratio and reconstructs the image by the compressed data. The experimental measurements of the samples are consistent with the theoretical and simulated results. This systematic scheme provides an innovative and effective way for designing miniaturized meta-devices to generate high-quality images, which relate to practical applications including high-density data storage, information security, and imaging.

Research on Modeling and Simulation of Fuel Cell Bus Power System Based on Forward-Backward energy flow balance method.

The modeling and simulation research of fuel cell buses' power system is an important part of accelerating the process of industrialization. This paper firstly analyzes the advantages and disadvantages of different topologies of power systems to determine the optimal configuration, then conducts the parameter matching study of the power system, derives and determines the main vehicle dynamics parameters and the total power demand of the power system, and further completes the parameter matching of the drive motor, gear ratio and power supply, as well as the design of the fuel cell stack. On the Matlab/Simulink platform, the forward-backward energy flow balance method is used to establish a fuel cell power system model divided into power calculation modules and power shunt modules. Finally, model simulation and comparative analysis was car-ried out. The simulation results of the power system model in this paper were compared with the data of the 2010 World Expo FCB real-vehicle test. The result shows that the simulation results of the dynamic system is in good agreement with the real vehicle data. The power system model conforms to the actual situation, has feasibility and high engineering value.

Research on innovative plug-in hybrid electric vehicle comprehensive energy consumption evaluation method based on statistic energy consumption.

The plug-in hybrid electric vehicle not only has the advantages of low emissions from electric vehicles, but also takes advantage of the high specific energy and high specific power of petroleum fuels, which can significantly improve the emissions and fuel economy of traditional vehicles. Studying its comprehensive energy consumption evaluation method is an important part of analyzing the economics of plug-in hybrid electric vehicles. This paper first puts forward the concept of statistical energy consumption and then proposes an innovative calculation method of plug-in hybrid electric vehicle energy consumption based on statistical energy consumption by referring to and analyzing the energy consumption test regulations of the United States, the European Union, and China. Given the two use case assumptions of charge depleting mode priority and charge sustaining mode only, considering the fuel consumption and the energy consumption that converts electrical energy consumption to fuel consumption, the probability density function of travel mileage distribution and energy consumption is derived. Finally, the interpretation and analysis of statistical energy consumption evaluation results are carried out.

Unified Chassis Control of Electric Vehicles Considering Wheel Vertical Vibrations.

In the process of vehicle chassis electrification, different active actuators and systems have been developed and commercialized for improved vehicle dynamic performances. For a vehicle system with actuation redundancy, the integration of individual chassis control systems can provide additional benefits compared to a single ABS/ESC system. This paper describes a Unified Chassis Control (UCC) strategy for enhancing vehicle stability and ride comfort by the coordination of four In-Wheel Drive (IWD), 4-Wheel Independent Steering (4WIS), and Active Suspension Systems (ASS). Desired chassis motion is determined by generalized forces/moment calculated through a high-level sliding mode controller. Based on tire force constraints subject to allocated normal forces, the generalized forces/moment are distributed to the slip and slip angle of each tire by a fixed-point control allocation algorithm. Regarding the uneven road, H∞ robust controllers are proposed based on a modified quarter-car model. Evaluation of the overall system was accomplished by simulation testing with a full-vehicle CarSim model under different scenarios. The conclusion shows that the vertical vibration of the four wheels plays a detrimental role in vehicle stability, and the proposed method can effectively realize the tire force distribution to control the vehicle body attitude and driving stability even in high-demanding scenarios.

A Novel V2V Cooperative Collision Warning System Using UWB/DR for Intelligent Vehicles.

The collision warning system (CWS) plays an essential role in vehicle active safety. However, traditional distance-measuring solutions, e.g., millimeter-wave radars, ultrasonic radars, and lidars, fail to reflect vehicles' relative attitude and motion trends. In this paper, we proposed a vehicle-to-vehicle (V2V) cooperative collision warning system (CCWS) consisting of an ultra-wideband (UWB) relative positioning/directing module and a dead reckoning (DR) module with wheel-speed sensors. Each vehicle has four UWB modules on the body corners and two wheel-speed sensors on the rear wheels in the presented configuration. An over-constrained localization method is proposed to calculate the relative position and orientation with the UWB data more accurately. Vehicle velocities and yaw rates are measured by wheel-speed sensors. An extended Kalman filter (EKF) is applied based on the relative kinematic model to combine the UWB and DR data. Finally, the time to collision (TTC) is estimated based on the predicted vehicle collision position. Furthermore, through UWB signals, vehicles can simultaneously communicate with each other and share information, e.g., velocity, yaw rate, which brings the potential for enhanced real-time performance. Simulation and experimental results show that the proposed method significantly improves the positioning, directing, and velocity estimating accuracy, and the proposed system can efficiently provide collision warning.

Electrical Switch of Poisson's Ratio in IV-VI Monolayers via Pseudophase Transitions.

The recent discovery of negative Poisson's ratio (NPR) in two-dimensional (2D) atomic crystals has stimulated extensive research interest in their intriguing physical properties. Here, via density functional theory (DFT) calculations, we reveal in the family of 2D IV-VI semiconductors that an iso-symmetry structure variation concerning the switch of the cation (IV) versus anion (VI) in the outermost layers leads to the change of sign of Poisson's ratio. Such iso-symmetry structural pseudo-phase transition can be induced by external strains, as well as electric fields, realizing the possibility of an electrically switchable Poisson effect. The phase transition process could involve a dynamic intermediate state with an alternative cation/anion switch in the frequencies of 2-3 THz according to the real-time time-dependent DFT (rt-TDDFT) calculations. The results open the way for studying pseudophases in 2D materials associated with sharply different physical properties, such as Poisson's ratio, for electromechanical and optoelectronic applications.

Exploring Heat-Response Mechanisms of MicroRNAs Based on Microarray Data of Rice Post-meiosis Panicle.

To explore heat response mechanisms of mircoRNAs (miRNAs) in rice post-meiosis panicle, microarray analysis was performed on RNA isolated from rice post-meiosis panicles which were treated at 40°C for 0 min, 10 min, 20 min, 60 min, and 2 h. By integrating paired differentially expressed (DE) miRNAs and mRNA expression profiles, we found that the expression levels of 29 DE-miRNA families were negatively correlated to their 178 DE-target genes. Further analysis showed that the majority of miRNAs in 29 DE-miRNA families resisted the heat stress by downregulating their target genes and a time lag existed between expression of miRNAs and their target genes. Then, GO-Slim classification and functional identification of these 178 target genes showed that (1) miRNAs were mainly involved in a series of basic biological processes even under heat conditions; (2) some miRNAs might play important roles in the heat resistance (such as osa-miR164, osa-miR166, osa-miR169, osa-miR319, osa-miR390, osa-miR395, and osa-miR399); (3) osa-miR172 might play important roles in protecting the rice panicle under the heat stress, but osa-miR437, osa-miR418, osa-miR164, miR156, and miR529 might negatively affect rice fertility and panicle flower; and (4) osa-miR414 might inhibit the flowering gene expression by downregulation of LOC_Os 05g51830 to delay the heading of rice. Finally, a heat-induced miRNA-PPI (protein-protein interaction) network was constructed, and three miRNA coregulatory modules were discovered.

High-Load Soft Grippers Based on Bionic Winding Effect.

The improvement of the load capacity of soft grippers has always been a challenge. To tackle this load capacity challenge, this work presents four novel types of high-load (HL) soft grippers that are bioinspired by bionic winding models. The winding models are found commonly in many animals and plants, where different winding patterns are used to grip different objects. Inspired by the winding models, we design four bionic winding structures that are driven by pneumatic artificial muscles (PAMs), and then four HL soft grippers are formed out of the winding structures. The inner cavities of the HL soft grippers contract after the PAMs are inflated, which enables objects to be wrapped to achieve gripping. Compared with most existing soft grippers, the HL soft grippers have a higher load capacity, and they can also grip various objects that have different shapes and stiffnesses without damaging them. In addition, in man-machine collaboration, operators can be in direct contact with them without being hurt. Our study helps lay the foundation for engineered systems with bionic winding structures.

MicroRNA-133a inhibits gastric cancer cells growth, migration, and epithelial-mesenchymal transition process by targeting presenilin 1.

Gastric cancer (GC) is one of the most common malignancies and a leading cause of cancer-related death worldwide. Accumulating evidence reported that microRNA (miR)-133a was involved in GC. This study aimed to investigate the function and mechanism of miR-133a in the development and progression of GC. The expression of miR-133a and presenilin 1 (PSEN1) in two GC cell lines, SGC-7901 and BGC-823, were inhibited and overexpressed by transient transfections. Thereafter, cell viability, migration, and apoptosis were measured by trypan blue exclusion assay, transwell migration assay, and flow cytometry assay, respectively. Dual-luciferase reporter assay was conducted to verify whether PSEN1 was a direct target of miR-133a. Furthermore, quantitative real-time polymerase chain reaction and Western blot analysis were mainly performed to assess the expression changes of epithelial-mesenchymal transition (EMT)-associated proteins, apoptosis-related proteins, and Notch pathway proteins. MiR-133a inhibitor significantly increased cell viability and migration, while miR-133a mimic decreased cell viability, migration, and induced apoptosis. miR-133a suppression accelerated transforming growth factor-ß1 (TGF-ß1)-induce EMT, as evidenced by upregulation of E-cadherin, and downregulation of N-cadherin, vimentin, and Slug. Of contrast, miR-133a overexpression blocked TGF-ß1-induce EMT by altering these factors. PSEN1 was a direct target of miR-133a, and suppression of PSEN1 abolished the promoting functions of miR-133 suppression on cell growth and metastasis. Moreover, PSEN1 inhibition decreased Notch 1, Notch 2, and Notch 3 protein expressions. This study demonstrates an antigrowth and antimetastasis role of miR-133a in GC cells. Additionally, miR-133a acts as a tumor suppressor may be via targeting PSEN1.

Overexpressing heat-shock protein OsHSP50.2 improves drought tolerance in rice.

KEY MESSAGE: OsHSP50.2, an HSP90 family gene up-regulated by heat and osmotic stress treatments, positively regulates drought stress tolerance probably by modulating ROS homeostasis and osmotic adjustment in rice. Heat-shock proteins (HSPs) serve as molecular chaperones for a variety of client proteins in abiotic stress response and play pivotal roles in protecting plants against stress, but the molecular mechanism remains largely unknown. Here, we report an HSP90 family gene, OsHSP50.2, which acts as a positive regulator in drought stress tolerance in rice (Oryza sativa). OsHSP50.2 was ubiquitously expressed and its transcript level was up-regulated by heat and osmotic stress treatments. Overexpression of OsHSP50.2 in rice reduced water loss and enhanced the transgenic plant tolerance to drought and osmotic stresses. The OsHSP50.2-overexpressing plants exhibited significantly lower levels of electrolyte leakage and malondialdehyde (MDA) and less decrease of chlorophyll than wild-type plants under drought stress. Moreover, the OsHSP50.2-overexpressing plants had significantly higher SOD activity under drought stress compared with the wild type. These results imply that OsHSP50.2 positively regulates drought stress tolerance in rice, probably through the modulation of reactive oxygen species (ROS) homeostasis. Additionally, the OsHSP50.2-overexpressing plants accumulated significantly higher content of proline than the wild type under drought stress, which contributes to the improved protection ability from drought stress damage via osmotic adjustment. Our findings reveal that OsHSP50.2 plays a crucial role in drought stress response, and it may possess high potential usefulness in drought tolerance improvement of rice.

The Arabidopsis AtUNC-93 Acts as a Positive Regulator of Abiotic Stress Tolerance and Plant Growth via Modulation of ABA Signaling and K+ Homeostasis.

Potassium (K+) is one of the essential macronutrients required for plant growth and development, and the maintenance of cellular K+ homeostasis is important for plants to adapt to abiotic stresses and growth. However, the mechanism involved has not been understood clearly. In this study, we demonstrated that AtUNC-93 plays a crucial role in this process under the control of abscisic acid (ABA). AtUNC-93 was localized to the plasma membrane and mainly expressed in the vascular tissues in Arabidopsis thaliana. The atunc-93 mutants showed typical K+-deficient symptoms under low-K+ conditions. The K+ contents of atunc-93 mutants were significantly reduced in shoots but not in roots under either low-K+ or normal conditions compared with wild type plants, whereas the AtUNC-93-overexpressing lines still maintained relatively higher K+ contents in shoots under low-K+ conditions, suggesting that AtUNC-93 positively regulates K+ translocation from roots to shoots. The atunc-93 plants exhibited dwarf phenotypes due to reduced cell expansion, while AtUNC-93-overexpressing plants had larger bodies because of increased cell expansion. After abiotic stress and ABA treatments, the atunc-93 mutants was more sensitive to salt, drought, osmotic, heat stress and ABA than wild type plants, while the AtUNC-93-overexpressing lines showed enhanced tolerance to these stresses and insensitive phenotype to ABA. Furthermore, alterations in the AtUNC-93 expression changed expression of many ABA-responsive and stress-related genes. Our findings reveal that AtUNC-93 functions as a positive regulator of abiotic stress tolerance and plant growth by maintaining K+ homeostasis through ABA signaling pathway in Arabidopsis.

Kinetics and mechanism of 17ß-estradiol chlorination in a pilot-scale water distribution systems.

The kinetics and mechanisms of 17ß-estradiol (E2) chlorination in water distribution systems (WDS) were studied. We examined the impacts of different factors, including pH, temperature, humic acid concentration and type, and flow velocity. The experimental results showed that the rate constants in beaker tests and WDS were described by a pseudo-first-order model. pH had the greatest impact on E2 chlorination in the beaker tests. However, temperature had the greatest impact on E2 chlorination in WDS. Mechanistic analysis of E2 chlorination showed that chlorine attacked E2 in three stages: 1) halogenation of the aromatic ring, 2) cleavage of the benzene moiety and chlorine or bromine substitution formation, and 3) formation of trihalomethanes (THMs) and halogenated acetic acids (HAAs) from phenolic intermediates through benzene ring opening with chlorine and/or bromine substitution of hydrogen on the carbon atoms. In the third stage, the concentrations of THMs and HAAs increased rapidly.

OsGL1-3 is involved in cuticular wax biosynthesis and tolerance to water deficit in rice.

Cuticular wax covers aerial organs of plants and functions as the outermost barrier against non-stomatal water loss. We reported here the functional characterization of the Glossy1(GL1)-homologous gene OsGL1-3 in rice using overexpression and RNAi transgenic rice plants. OsGL1-3 gene was ubiquitously expressed at different level in rice plants except root and its expression was up-regulated under ABA and PEG treatments. The transient expression of OsGL1-3-GFP fusion protein indicated that OsGL1-3 is mainly localized in the plasma membrane. Compared to the wild type, overexpression rice plants exhibited stunted growth, more wax crystallization on leaf surface, and significantly increased total cuticular wax load due to the prominent changes of C30-C32 aldehydes and C30 primary alcohols. While the RNAi knockdown mutant of OsGL1-3 exhibited no significant difference in plant height, but less wax crystallization and decreased total cuticular wax accumulation on leaf surface. All these evidences, together with the effects of OsGL1-3 on the expression of some wax synthesis related genes, suggest that OsGL1-3 is involved in cuticular wax biosynthesis. Overexpression of OsGL1-3 decreased chlorophyll leaching and water loss rate whereas increased tolerance to water deficit at both seedling and late-tillering stages, suggesting an important role of OsGL1-3 in drought tolerance.

Transcriptome profile reveals heat response mechanism at molecular and metabolic levels in rice flag leaf.

Flag leaf is one of the key photosynthesis organs during rice reproductive stage. A time course microarray analysis of rice flag leaf was done after 40°C treatment for 0 min, 20 min, 60 min, 2h, 4h, and 8h. The identified significant heat responsive genes were mainly involved in transcriptional regulation, transport, protein binding, antioxidant, and stress response. KMC analysis discovered the time-dependent gene expression pattern under heat. MapMan analysis demonstrated that, under heat treatment, Hsp genes and genes involved in glycolysis and ubiquitin-proteasome were enhanced, and genes involved in TCA, carotenoid, dihydroflavonol and anthocyanin metabolisms and light-reaction in the photosynthesis were widely repressed. Meanwhile, some rate-limiting enzyme genes in shikimate, lignin, and mevalonic acid metabolisms were up-regulated, revealing the importance of maintaining specific secondary metabolites under heat stress. The present study increased our understanding of heat response in rice flag leaf and provided good candidate genes for crop improvement.

Heat shock factor OsHsfB2b negatively regulates drought and salt tolerance in rice.

KEY MESSAGE: Expression of OsHsfB2b was strongly induced by heat, salt, ABA and PEG treatments. Drought and salt tolerances were significantly decreased by OsHsfB2b overexpression, but were enhanced by RNA interference. ABSTRACT: Plants have more than 20 heat shock factors (Hsfs) that were designated class A, B, and C. Many members of Class A Hsfs were characterized as activators of transcription, but the functional roles of class B and C Hsfs have not been fully recognized. OsHsfB2b is a member of class B Hsfs in rice (Oryza sativa). Expression of OsHsfB2b was strongly induced by heat, salt, abscisic acid (ABA) and polyethylene glycol (PEG) treatments but was almost not affected by cold stress. Drought and salt tolerances were significantly decreased in OsHsfB2b-overexpressing transgenic rice, but were enhanced in the OsHsfB2b-RNAi transgenic rice. Under drought stress, the OsHsfB2b-overexpressing transgenic rice exhibited increased relative electrical conductivity (REC) and content of malondialdehyde (MDA) and decreased proline content compared with the wild type, while the lower REC and MDA content and increased proline content were found in the OsHsfB2b-RNAi transgenic rice. These results suggest that OsHsfB2b functions as a negative regulator in response to drought and salt stresses in rice, with its existing B3 repression domain (BRD) that might be necessary for the repressive activity. The present study revealed the potential value of OsHsfB2b in genetic improvement of rice.

Sequencing, de novo assembly, annotation and SSR and SNP detection of sabaigrass (Eulaliopsis binata) transcriptome.

Eulaliopsis binata is one of the best fiber grass plants for its high fiber quality and production. Large scale trancriptome sequencing of E. binata was first performed using mixed leaf samples of 20 wild clusters. A total of 26,438,832 clean reads were generated and were assembled into 59,134 isogenes with an average length of 845bp. BLAST against the NCBI non-redundant protein, KEGG and GO databases has classified these isogenes into functional categories for understanding gene functions and regulation pathways. Only 15.0% of the assembled isogenes were similar to known proteins and 24.4% has no hits in the nr protein database. The total isogenes and 5306 highly expressed isogenes were performed by BLASTx with the MAIZEWALL, the cell wall navigator and the PlantTFDB databases. A total of 6681 simple sequence repeats (SSRs) and 147,177 single nucleotide polymorphisms (SNPs) were detected in the isogenes and 5723 pairs of SSR primers were designed.

Over-expression of OsHsfA7 enhanced salt and drought tolerance in transgenic rice.

Heat shock proteins play an important role in plant stress tolerance and are mainly regulated by heat shock transcription factors (Hsfs). In this study, we generated transgenic rice over-expressing OsHsfA7 and carried out morphological observation and stress tolerance assays. Transgenic plants exhibited less, shorter lateral roots and root hair. Under salt treatment, over-expressing OsHsfA7 rice showed alleviative appearance of damage symptoms and higher survival rate, leaf electrical conductivity and malondialdehyde content of transgenic plants were lower than those of wild type plants. Meanwhile, transgenic rice seedlings restored normal growth but wild type plants could not be rescued after drought and re-watering treatment. These findings indicate that over-expression of OsHsfA7 gene can increase tolerance to salt and drought stresses in rice seedlings.