Comparison study of surface-initiated hydrogel coatings with distinct side-chains for improving biocompatibility of polymeric heart valves.

Polymeric heart valves (PHVs) present a promising alternative for treating valvular heart diseases with satisfactory hydrodynamics and durability against structural degeneration. However, the cascaded coagulation, inflammatory responses, and calcification in the dynamic blood environment pose significant challenges to the surface design of current PHVs. In this study, we employed a surface-initiated polymerization method to modify polystyrene-block-isobutylene-block-styrene (SIBS) by creating three hydrogel coatings: poly(2-methacryloyloxy ethyl phosphorylcholine) (pMPC), poly(2-acrylamido-2-methylpropanesulfonic acid) (pAMPS), and poly(2-hydroxyethyl methacrylate) (pHEMA). These hydrogel coatings dramatically promoted SIBS's hydrophilicity and blood compatibility at the initial state. Notably, the pMPC and pAMPS coatings maintained a considerable platelet resistance performance after 12 h of sonication and 10 000 cycles of stretching and bending. However, the sonication process induced visible damage to the pHEMA coating and attenuated the anti-coagulation property. Furthermore, the in vivo subcutaneous implantation studies demonstrated that the amphiphilic pMPC coating showed superior anti-inflammatory and anti-calcification properties. Considering the remarkable stability and optimal biocompatibility, the amphiphilic pMPC coating constructed by surface-initiated polymerization holds promising potential for modifying PHVs.

A Ca2+ sensor BraCBL1.2 involves in BraCRa-mediated clubroot resistance in Chinese cabbage.

Clubroot disease caused by Plasmodiophora brassicae (P. brassicae) severely threatens the cultivation of Cruciferous plants, especially Chinese cabbage. Recently, resistance genes in plants have been reported to encode for a Ca2+-permeable channel in the plasma membrane, which can mediate the cytosolic Ca2+ increase in plant cells upon pathogen attack. However, the downstream Ca2+ sensor and decoder are still unknown. In this study, we identified the virulent and avirulent P. brassicae isolates (Pbs) of two near isogenic lines, CR 3-2 and CS 3-2, with CR 3-2 harboring clubroot resistant gene BraCRa. The transcriptomic analysis was then conducted with CR 3-2 after inoculating with virulent isolate PbE and avirulent isolate Pb4. From the differentially expressed genes of transcriptomic data, we identified a Ca2+-sensor encoding gene, BraCBL1.2, that was highly induced in CR 3-2 during infection by Pb4 but not by PbE. Moreover, GUS histochemical staining and subcellular localization analysis revealed that BraCBL1.2 was specifically expressed in the root hair cells of Arabidopsis and encoded a putative Ca2+ sensor localized in the plasma membrane. We also developed an assay to investigate the BraCRa-mediated hypersensitive response (HR) in tobacco leaves. The results suggest that BraCBL1.2 is involved in the BraCRa-mediated plant ETI immune response against P. brassicae. In addition, we verified that overexpression of BraCBL1.2 enhanced clubroot resistance in Arabidopsis. Collectively, our data identified the involvement of a Ca2+ sensor in BraCRa-mediated clubroot resistance in Chinese cabbage, providing a theoretical basis for further research on the resistance of Chinese cabbage to P. brassicae.

The relationship between atrial fibrillation and NLRP3 inflammasome: a gut microbiota perspective.

Atrial fibrillation (AF) is a common clinical arrhythmia whose pathogenesis has not been fully elucidated, and the inflammatory response plays an important role in the development of AF. The inflammasome is an important component of innate immunity and is involved in a variety of pathophysiologic processes. The NLRP3 inflammasome is by far the best studied and validated inflammasome that recognizes multiple pathogens through pattern recognition receptors of innate immunity and mediates inflammatory responses through activation of Caspase-1. Several studies have shown that NLRP3 inflammasome activation contributes to the onset and development of AF. Ecological dysregulation of the gut microbiota has been associated with the development of AF, and some evidence suggests that gut microbiota components, functional byproducts, or metabolites may induce or exacerbate the development of AF by directly or indirectly modulating the NLRP3 inflammasome. In this review, we report on the interconnection of NLRP3 inflammasomes and gut microbiota and whether this association is related to the onset and persistence of AF. We discuss the potential value of pharmacological and dietary induction in the management of AF in the context of the association between the NLRP3 inflammasome and gut microbiota. It is hoped that this review will lead to new therapeutic targets for the future management of AF.

Hybridized Triboelectric-Electromagnetic Aeolian Vibration Generator as a Self-Powered System for Efficient Vibration Energy Harvesting and Vibration Online Monitoring of Transmission Lines.

In this work, based on the triboelectric-electromagnetic working principle, a comprehensive strategy appropriately hybridizes a multilayered elastic structure TENG (ME-TENG) and a double-electromagnetic generator (EMG) for efficient aeolian vibration energy harvesting and vibration state monitoring. The ME-TENG with the feature of elasticity is integrated with a movable plate embedded with a magnet as the counterweight, which acts as a spring-like mass system in response to external vibration excitation, making the inseparable integrity of the TENG and EMG. The basic hybridized triboelectric-electromagnetic aeolian vibration generator (HAVG) consisting of ME-TENG and double-EMGs in terms of structural parameters and response characteristics is first optimized and discussed, thereby the efficient vibration energy harvesting and effective vibration state response can be further improved through the mutual complementarity of TENG and EMG. Furthermore, the self-powered capacity of the HAVG in terms of LED arrays and a wireless ambient temperature and humidity monitoring system is verified through the hybrid charging strategy of TENG and EMG modules and the combination of HVAG and energy management circuits, benefiting from the sophisticated-designed structure and excellent output performance of the HAVG. Importantly, a self-powered aeolian vibration monitoring system is established and demonstrated for vibration-state sensing and abnormal vibration alarm. This work demonstrates a novel strategy for energy harvesting and state sensing of overhead transmission line aeolian vibration, which not only reveals TENG-EMG promising potential for energy harvesting for aeolian vibration, but also provides valuable guidance for the construction of a self-powered online-monitoring system for transmission lines.

Ratiometric Fluorescent Sensor Based on Tb(III) Functionalized Metal-Organic Framework for Formic Acid.

Formic acid is a common chemical raw material, the effective detection of which is of importance to food safety and environmental quality. In this work, the lanthanide functionalized dual-emission metal-organic framework (TH25) was prepared as a ratiometric fluorescent sensor for formic acid. This ratiometric sensor has a good detection performance with high selectivity, sensitivity, and reproducibility. Together with a low limit of detection of 2.1 ppm, these characters promise the ability to sense at low levels as well as a practical detection ability. This work provides ideas for the design and synthesis of effective chemical sensors for organic acids.

Improving consumer stickiness in livestream e-commerce: A mixed-methods study.

With the continuous development and improvement of Internet media technologies in China, the influence of livestream e-commerce is becoming increasingly prominent, and an increasing number of people are engaging in consumption activities in this field. It is important to study consumer stickiness in livestream e-commerce to promote economic structure adjustment and innovation-driven development. Therefore, in this study, we adopted the expectation confirmation theory (ECT) as the theoretical framework and analyzed the ECT and stickiness. The study considered satisfaction as the previous influencing factor of user and consumer stickiness, replaced the continuance intention in the expectation confirmation model with consumer stickiness as the explanatory variable, introduced the variable of perceived playfulness as the value perception after user experience, and established a consumer stickiness factors model. A total of 262 valid questionnaires were collected in this study, and SmartPLS analysis along with interviews were used to justify the limitations of data analysis. The results of the study demonstrated a significant effect of perceived usefulness and confirmation on satisfaction, a significant effect of confirmation on perceived usefulness, a significant effect of satisfaction on stickiness, and a significant effect of confirmation on perceived playfulness. Based on findings from the data analysis and interviews, we further proposed rationalized recommendations, and aimed to provide some theoretical guidance for future research on live streaming.

[Research progress in nitric oxide biosynthesis, degradation and function in fungi].

Nitric oxide is a highly reactive molecule with dichotomous regulatory roles in numerous physiological and pathological events. It has been recognized as an intra-and inter-cellular signaling molecule in animals, plants and microorganisms. Recent research data indicate that fungi are capable of synthesizing nitric oxide. Appropriate amounts of nitric oxide play important biological roles in fungal cells. However, excessive amounts of nitric oxide will damage cells and evoke apoptosis. Nitric oxide regulates the synthesis of cGMP, an important intracellular secondary messenger molecule, involved in the control of a variety of signal transduction pathways in fungal cells. Nitric oxide regulates the cellular development, morphogenesis, sporulation, spore germination, reproduction and apoptosis in fungi. Nitric oxide affects the physiological function of fungi throughout the life cycle. Although the mechanism of nitric oxide in plants and animals has been widely studied, there are limited reports about nitric oxide in fungi; and further investigation is needed to illustrate the nitric oxide synthesis, degradation pathways and the mechanism of signal transduction in the fungal system.