A comparison of students' preferences for face-to-face and online laboratory sessions: insights from students' perception of their learning experiences in an immunology course.

The COVID-19 global pandemic has prompted educators in universities to reconsider their teaching methods, mainly due to the social distancing measures imposed within the classroom settings. On the other hand, the growing importance of continuing education opportunities for adult learners after graduation has seen the need to transform traditional teaching modes that primarily depend on face-to-face interaction into virtual modes, which are deemed more time- and cost-efficient. These major shifts in social and economic developments have a significant impact on the evolution of curriculum planning in higher education. Education that has scientific inquiry components inevitably comes into question, as conventional beliefs that experiments should be hands-on and will not be as effective if conducted virtually cast doubts on the move to the online space. This paper discusses the background of an impending shift in a university's approach to more online-based laboratory classes in an immunology course, as well as the exploration of the potential of conducting online laboratory experiments based on student perceptions.

Improved Endurance of Ferroelectric Hf0.5Zr0.5O2 Using Laminated-Structure Interlayer.

In this article, the endurance characteristic of the TiN/HZO/TiN capacitor was improved by the laminated structure of a ferroelectric Hf0.5Zr0.5O2 thin film. Altering the HZO deposition ratio, the laminated-structure interlayer was formed in the middle of the HZO film. Although small remanent polarization reduction was observed in the capacitor with a laminated structure, the endurance characteristic was improved by two orders of magnitude (from 106 to 108 cycles). Moreover, the leakage current of the TiN/HZO/TiN capacitor with the laminated-structure interlayer was reduced by one order of magnitude. The reliability enhancement was proved by the Time-Dependent Dielectric Breakdown (TDDB) test, and the optimization results were attributed to the migration inhibition and nonuniform distribution of oxygen vacancies. Without additional materials and a complicated process, the laminated-structure method provides a feasible strategy for improving HZO device reliability.

BMP9 promotes autophagy and inhibits migration and invasion in breast cancer cells through the c-Myc/SNHG3/mTOR signaling axis.

We previously reported that BMP9 inhibited breast cancer progression. However, the precise molecular mechanism is still unknown. Based on our RNA-sequencing (RNA-seq) results, BMP9 significantly down-regulated the expression of long non-coding RNA SNHG3. Exogenous BMP9 promoted autophagy and inhibited migration and invasion in MDA-MB-231 cells, which was effectively blunted by SNHG3 overexpression. Interestingly, SNHG3 was negatively connected with autophagy. Knockdown of SNHG3 induced autophagy by increasing the formation of autophagic vacuoles and thus inhibited the migration and invasion of MDA-MB-231 cells. Mechanically, BMP9-SNHG3 activated AMPK, AKT and mTOR signaling pathways to induce autophagy and inhibit migration and invasion. Meanwhile, BMP9 regulated SNHG3 transcription by suppressing c-Myc entry into the nucleus. In conclusion, BMP9 promotes autophagy and inhibits migration and invasion in breast cancer cells through the c-Myc/SNHG3/mTOR signaling axis, which might offer a fresh perspective on BMP9's breast cancer-inhibiting properties.

The Evaluation Value of Diffusion-Weighted Imaging for Brain Injury in Patients after Deep Hypothermic Circulatory Arrest.

Objective: Cerebral complications may occur after surgery with deep hypothermic circulatory arrest (DHCA). Diffusion-weighted imaging (DWI) has shown promising results in detecting early changes of cerebral ischemia. However, studies in human models are limited. Here, we examined the significance of DWI for detecting brain injury in postoperative patients after DHCA. Methods: Twelve patients who had undergone selective cerebral perfusion with DHCA were enrolled. All patients underwent magnetic resonance imaging (MRI) examinations before and after the operation with T1-weighted phase (T1W) and T2-weighted phase (T2W). Magnetic resonance angiography (3D TOF) was applied to observe intracranial arterial communication situations. DWI was employed to calculate the apparent diffusion coefficient (ADC) values. The neurocognitive function of patients was assessed preoperatively and postoperatively using the Montreal Cognitive Assessment Scale (MoCA), Hamilton Depression Scale (HAMD), and Hamilton Anxiety Scale (HAMA). Results: The ADC values of the whole brain of patients after surgery were significantly higher than before surgery (P = 0.003). However, no significant difference in the ADC values of other regions before and after the operation was observed. There was no significant effect on the postoperative cognitive function of patients after surgery, but visual-spatial and executive abilities were significantly reduced, while psychological anxiety (P = 0.005) and depression levels (P < 0.05) significantly increased. Correlation analysis revealed a significant association between ADC change values and depression change values (P < 0.05). Conclusion: DHCA demonstrated no significant effect on the cognitive function of patients but could affect the mood of patients. On the other hand, DWI demonstrated promising efficiency and accuracy in evaluating brain injury after DHCA.

Experimental demonstration of pseudo-pilots-aided Gaussian basis expansion-based phase noise suppression scheme for CO-OFDM.

In this paper, we propose a novel phase noise suppression scheme called pseudo-pilots-aided Gaussian basis expansion (PS-GBE) for coherent optical orthogonal frequency division multiplexing (CO-OFDM) system. Integrating with the pseudo-pilots, the Gaussian basis can suppress and fit phase noise effectively with high spectral efficiency (SE). We experimentally demonstrate the proposed scheme in 200-Gb/s optical QPSK-OFDM superchannel and 400-Gb/s optical 16QAM-OFDM superchannel. According to the experimental results, the proposed PS-GBE has a phase noise suppression performance similar to the GBE scheme. However, different from the GBE scheme, fewer pilots are needed for phase noise estimation in PS-GBE. Hence, the proposed PS-GBE can achieve higher SE. Compared to the pseudo-pilots-aided orthogonal basis expansion-based (PS-OBE) phase noise suppression and traditional common phase noise compensation (CPEC) methods, the proposed PS-GBE can get a significant performance enhancement. In the QPSK-OFDM system, a Q-factor improvement of 1.1 dB is obtained compared to the PS-OBE method. In the 16QAM-OFDM system, the BER is enhanced from 1.00E-03 to 2.05E-04. Further simulation results indicate that our proposed PS-GBE scheme can effectively increase laser linewidth tolerance. The proposed scheme can be applied to CO-OFDM and extended to coherent optical filterbank multicarrier (CO-FBMC) and coherent optical single carrier frequency domain multiplexing (CO-SCFDM) transmission system.

Gaussian basis expansion phase noise suppression method for CO-OFDM systems.

In this paper, we propose a novel phase noise suppression method based on Gaussian basis expansion (GBE) for CO-OFDM systems. We analyze the basic phase noise suppression principle of GBE and then demonstrate it in optical OFDM transmission systems. Compared with common phase compensation (CPE), orthogonal basis expansion (OBE) and eigenvector basis expansion (EBE) schemes, the proposed GBE scheme has better phase noise fitting ability with similar computation complexity. Futhermore, no additional back to back (BTB) pre-training is needed for the GBE scheme. Performance improvements by the GBE is experimental validated in the QPSK/16-QAM OFDM system with different transmission scenarios (different number of pilots and input power). In the QPSK-OFDM system, after 160 km SSMF transmission, a Q-factor improvement of 1.7 dB and 0.5 dB are achieved compared with the CPE and OBE schemes at the optimum input power of -4 dBm, respectively. In the 16-QAM-OFDM system, the measured BER improved from 8.21×10-4 to 2.36×10-4 with the GBE scheme. To further verify the effectiveness of the GBE scheme, we change the laser linewidth and measure the long transmission distance performance by simulation, the results show that the GBE scheme can effectively increase the laser linewidth tolerance and extend transmission distance. When the linewidth is 2-MHz, the proposed GBE scheme can extend the transmission distance from 1120 km to 1540 km at the BER of 10-4. Experimental and simulation results show that the proposed GBE scheme is a promising alternative phase noise suppression for CO-OFDM system.

Multi scrolls chaotic encryption scheme for CO-OFDM-PON.

In this paper, we propose and experimentally demonstrate a novel multi scrolls chaotic encryption scheme for CO-OFDM-PON. We analyze the principle of 3-dimension encryption scheme and discuss its encryption complexity. Compared with the previous hyper Chen chaotic encryption scheme, the proposed encryption algorithm can realize dynamic constellation point mapping of QAM signal with lower encryption complexity. We also compare the transmission performances of the two chaotic encryption schemes. The results show that the proposed multi scrolls scheme has better BER performance because it can decrease the peak to average power ratio (PAPR) of OFDM. What is more, the proposed encryption scheme is very sensitive to the initial secure key and a tiny discrepancy as small as 10-17 would lead to a completely different sequence. The high sensibility to the initial value can effectively increase encryption level and the key space of the multi scrolls encryption scheme is 106 times of that hyper Chen. Further, to verify the effectiveness of the proposed encryption algorithm, encrypted transmission of a digital picture in 80 km SSMF is carried out.

Silencing of long non-coding RNA Sox2ot inhibits oxidative stress and inflammation of vascular smooth muscle cells in abdominal aortic aneurysm via microRNA-145-mediated Egr1 inhibition.

Long non-coding RNAs (lncRNAs) have been largely reported to contribute to the development and progression of abdominal aortic aneurysm (AAA), a common vascular degenerative disease. The present study was set out with the aim to investigate the possible role of lncRNA Sox2ot in the development of AAA. In this study, we found that lncRNA Sox2ot and early growth response factor-1 (Egr1) were highly expressed, while microRNA (miR)-145 was poorly expressed in Ang II-induced AAA mice and oxidative stress-provoked vascular smooth muscle cell (VSMC) model. Egr1 was a potential target gene of miR-145, and lncRNA Sox2ot could competitively bind to miR-145 to upregulate Egr1 expression. Overexpression of miR-145-5p was found to attenuate oxidative stress and inflammation by inhibiting Egr1 both in vivo and in vitro, which was counteracted by lncRNA Sox2ot. Taken together, the present study provides evidence that downregulation of lncRNA Sox2ot suppressed the expression of Egr1 through regulating miR-145, thus inhibiting the development of AAA, highlighting a theoretical basis for AAA treatment.

Increased Tim-3 expression on TILs during treatment with the Anchored GM-CSF vaccine and anti-PD-1 antibodies is inversely correlated with response in prostate cancer.

Programmed death receptor-1 (PD-1) and T cell immunoglobulin and mucin domain-containing protein-3 (Tim-3) play important roles in tumor immune evasion. PD-1 blockade could produce an effective antitumor effect in many solid tumors except prostate cancer (PCa) because of rare programmed death ligand-1 (PD-L1) expression on PCa cells. Streptavidin (SA)-GM-CSF surface-anchored tumor cell (Anchored GM-CSF) vaccines could increase the number of tumor-infiltrating lymphocytes (TILs) and induce specific antitumor immune responses. The Anchored-GM-CSF vaccine and anti-PD-1 antibodies exerted synergistic effects in mouse models of PCa metastasis. However, the response rate was low due to the presence of other negative regulatory pathways. Tim-3 expression could be upregulated at resistance to combination therapy with anti-PD-1 antibodies and the Anchored GM-CSF vaccine. Sequential administration of anti-PD-1 and anti-Tim-3 antibodies could further improve the efficacy of the Anchored GM-CSF vaccine therapy, and tumor regression was noted in over 60% of animals. This triple therapy improved the specific cytotoxic activity, proliferation and secretion of CD8+ TILs and reduced the production of tumor-promoting cytokines. These findings indicated that this triple therapy could induce a robust antitumor immune response in mouse models of PCa.

Wavelength-interleaved MDM-WDM transmission over weakly-coupled FMF.

Recently mode-division-multiplexing (MDM) has been widely investigated to enhance fiber optics capacity, in which modes or mode groups in few-mode fiber (FMF) or multi-mode fiber (MMF) are exploited as different spatial channels for data transmission. For short-reach applications, significantly reducing inter-spatial-channel crosstalk to avoid coherent detection and multiple-input-multiple-output (MIMO) equalization is preferred. Currently most studies focus on the design of weakly-coupled FMFs and mode (de)multiplexers. Alternatively, in this work, a wavelength-interleaved (WI) scheme is proposed to mitigate inter-spatial-channel crosstalk by optimizing the design of direct detection (DD) MDM and wavelength-division-multiplexing (WDM) system. In weakly-coupled MDM systems, crosstalk mainly comes from the adjacent spatial channels, and the signal-to-crosstalk beat interference (SCBI) constitutes main crosstalk impairment after square-law detection. The WI scheme interleaves the WDM grids in adjacent spatial channels by half WDM channel spacing and uses an electrical low-pass filtering (ELPF) to remove out-of-band SCBI. The effectiveness of SCBI suppression is theoretically analyzed. The feasibility of WI scheme is experimentally verified by 3-mode 3-wavelength MDM-WDM transmission over 500-m OM3 MMF. Enabled by WI scheme, record 120-km 10G-per-channel MDM-WDM transmission over 2-mode FMF without MIMO equalization is successfully demonstrated. The WI scheme is promising to enhance the performance of short reach or even metro MDM optics.

Wideband tunable optoelectronic oscillator based on the deamplification of stimulated Brillouin scattering.

A wideband tunable optoelectronic oscillator (OEO) based on the deamplification of stimulated Brillouin scattering (SBS) is proposed and experimentally demonstrated. A tunable single passband microwave photonic filter (MPF) utilizing phase modulation and SBS deamplification is used to realize the tunability of the OEO. Theoretical analysis of the MPF and phase noise performance of the OEO are presented. The frequency response of the MPF is determined by the + 1st sideband attenuation due to SBS deamplification and phase shift difference between the two sidebands due to chromatic dispersion and SBS. The close-in (< 1 MHz) phase noise of the proposed OEO is shown to be dominated by the laser frequency noise via phase shift of SBS. The conversion of the laser frequency noise to the close-in phase noise of the proposed OEO is effectively reduced compared with the OEO based on amplification by SBS. Tunable 7 to 40 GHz signals are experimentally obtained. The single-sideband (SSB) phase noise at 10 kHz offset is -128 dBc/Hz for 10.30 GHz signal. Compared with the OEO based on SBS amplification, the proposed OEO can achieve a phase noise performance improvement beyond 20 dB at 10 kHz offset. The maximum frequency and power drifts at 10.69 GHz are within 1 ppm and 1.4 dB during 1000 seconds, respectively. To achieve better close-in phase noise performance, lower frequency noise laser and higher pump power are preferred. The experimental results agree well with the theoretical models.

Cost effective wavelength reused MDM system for bidirectional mobile fronthaul.

In this paper, we propose a cost-effective wavelength-reused mode-division-multiplexing (MDM) system for high speed symmetrical bidirectional mobile fronthaul application. At the base band unit (BBU) pool, one of the spatial modes is used to transmit signal carrier while the others are used for downstream (DS) signal channels. At the remote radio unit (RRU) side, the signal carrier is split and reused as modulation carrier for all the upstream (US) signal channels after mode demultiplexing. Thanks to the low mode crosstalk characteristic of the mode multiplexer/demultiplexer (MUX/DEMUX) and few-mode fiber (FMF), the signal carrier and each signal channel can be effectively separated. The spectral efficiency (SE) is significantly enhanced when multiple spatial channels are used. Compared with other wavelength reused scheme in which the downstream and upstream be modulated in orthogonal dimension, the modulation format of both directions are independent in the proposed wavelength reused MDM system. Therefore, it can easily achieve symmetrical bidirectional transmission without residual re-modulation crosstalk. The proposed scheme is scalable to multi-wavelength application when wavelength MUX/DEMUX is utilized. With the proposed scheme, we demonstrate a proof of concept intensity modulated 4 × 25-Gb/s 16-QAM orthogonal frequency division multiplexing (OFDM) transmission over 10-km FMF using low modal-crosstalk two-mode FMF and MUX/DEMUX with error free operation. The downstream receiver sensitivity is -21 dBm while the upstream receiver sensitivity is -18 dBm for bidirectional transmission. Due to the Rayleigh backscattering and other spurious reflections, the upstream suffers 2 dB power penalty compared with unidirectional transmission without downstream. To mitigate bidirectional transmission impairments, we propose a simple and effective method to suppress Rayleigh backscattering by shifting the downstream subcarrier frequency. A receiver sensitivity improvement of up to 2.5 dB is achieved for upstream with different downstream power.

Tunable optical multicasting of PDM-OFDM signals by novel polarization-interleaved multi-pump FWM scheme.

Optical multicasting that supports point-to-multipoint traffic replication can be one of the necessary techniques in next-generation all-optical elastic networks. In this paper, we propose an optical multicasting approach for polarization-division-multiplexing (PDM) orthogonal frequency division multiplexing (OFDM) signals based on a novel polarization-interleaved multi-pump (PIMP) four-wave mixing (FWM) scheme in highly nonlinear fiber (HNLF). Besides format transparency and the support of PDM signals, the scheme further enables wide spectral tunability of generated replicas. The pump frequency arrangement for the scheme is presented, which successfully prevents the replicas from being superimposed by unwanted FWM components during tuning. We experimentally demonstrate multicasting operation of a 3-band 100-Gb/s PDM-OFDM signal. With different input positions, 1.4 and 1.6 Terahertz tuning ranges of four replicas are achieved with Q-factor performance better than the forward error correction threshold. Tunable replica spacing from 100-GHz to 250-GHz are also verified. In addition, the scalability of the scheme is demonstrated via 5-pump multicasting, successfully generating a total of 14 replicas.

Quantification of MDL-induced signal degradation in MIMO-OFDM mode-division multiplexing systems.

Mode-division multiplexing (MDM) transmission over few-mode optical fiber has emerged as a promising technology to enhance transmission capacity, in which multiple-input-multiple-output (MIMO) digital signal processing (DSP) after coherent detection is used to demultiplex the signals. Compared with conventional single-mode systems, MIMO-MDM systems suffer non-recoverable signal degradation induced by mode-dependent loss (MDL). In this paper, the MDL-induced signal degradation in orthogonal-frequency-division-multiplexing (OFDM) MDM systems is theoretically quantified in terms of mode-average error vector magnitude (EVM) through frequency domain norm analysis. A novel scalar MDL metric is proposed considering the probability distribution of the practical MDM input signals, and a closed-form expression for EVM measured after zero-force (ZF) MIMO equalization is derived. Simulation results show that the EVM estimations utilizing the novel MDL metric remain unbiased for unrepeated links. For a 6 × 100 km 20-mode MDM transmission system, the estimation accuracy is improved by more than 90% compared with that utilizing traditional condition number (CN) based MDL metric. The proposed MDL metric can be used to predict the MDL-induced SNR penalty in a theoretical manner, which will be beneficial for the design of practical MIMO-MDM systems.

Novel MDM-PON scheme utilizing self-homodyne detection for high-speed/capacity access networks.

In this paper, we propose a cost-effective, energy-saving mode-division-multiplexing passive optical network (MDM-PON) scheme utilizing self-homodyne detection for high-speed/capacity access network based on low modal-crosstalk few-mode fiber (FMF) and all-fiber mode multiplexer/demultiplexer (MUX/DEMUX). In the proposed scheme, one of the spatial modes is used to transmit a portion of signal carrier (namely pilot-tone) as the local oscillator (LO), while the others are used for signal-bearing channels. At the receiver, the pilot-tone and the signal can be separated without strong crosstalk and sent to the receiver for coherent detection. The spectral efficiency (SE) is significantly enhanced when multiple spatial channels are used. Meanwhile, the self-homodyne detection scheme can effectively suppress laser phase noise, which relaxes the requirement for the lasers line-width at the optical line terminal or optical network units (OLT/ONUs). The digital signal processing (DSP) at the receiver is also simplified since it removes the need for frequency offset compensation and complex phase correction, which reduces the computational complexity and energy consumption. Polarization division multiplexing (PDM) that offers doubled SE is also supported by the scheme. The proposed scheme is scalable to multi-wavelength application when wavelength MUX/DEMUX is utilized. Utilizing the proposed scheme, we demonstrate a proof of concept 4 × 40-Gb/s orthogonal frequency division multiplexing (OFDM) transmission over 55-km FMF using low modal-crosstalk two-mode FMF and MUX/DEMUX with error free operation. Compared with back to back case, less than 1-dB Q-factor penalty is observed after 55-km FMF of the four channels. Signal power and pilot-tone power are also optimized to achieve the optimal transmission performance.

Experimental demonstration of EON node supporting reconfigurable optical superchannel multicasting.

Elastic optical networks (EON) based on optical superchannel enables higher spectral flexibility, in which the network nodes should provide multiple all-optical functionalities to manipulate bandwidth-variable data traffic. In this paper, we propose and demonstrate an EON node structure supporting reconfigurable optical superchannel multicasting. The node structure incorporates a shared multicasting module, which performs reconfigurable selection of target incoming/outgoing superchannels/replicas and leverages a group of nonlinear devices to satisfy multiple multicast requests. Moreover, an optical comb is utilized to efficiently provide and manage all pump resources for multicasting with potential cost reduction and phase noise inhibition. Based on the node structure, we experimentally demonstrate polarization division multiplexing (PDM) superchannel multicasting scenarios with different replica amount, input/output locations, and modulation formats. Less than 0.7 dB optical signal-to-noise ratio (OSNR) penalties are demonstrated in multiple multicasting scenarios.

Optimization of pH values to formulate the bireagent kit for serum uric acid assay.

A new formulation of the bireagent kit for serum uric acid assay was developed based on the effects of pH on enzyme stability. At 4 °C, half-lives of uricases from Bacillus fastidious and Arthrobacter globiforms were longer than 15 months at pH 9.2, but became shorter at pH below 8.0; half-lives of ascorbate oxidase and peroxidase were comparable at pH 6.5 and 7.0, but became much shorter at pH higher than 7.4. In the new formulation of the bireagent kit, Reagent A contained peroxidase, 4-aminoantipyrine, and ascorbate oxidase in 50 mM phosphate buffer at pH 6.5; Reagent B contained B. fastidious or A. globiforms uricase in 50 mM sodium borate buffer at pH 9.2; Reagents A and B were mixed at 4:1 to produce a final pH from 7.2 to 7.6 for developing a stable color. The new bireagent kit consumed smaller quantities of three enzymes for the same shelf life. With the new bireagent kit, there were linear responses of absorbance at 546 nm to uric acid up to 34 mM in reaction mixtures and a good correlation of uric acid levels in clinical sera with those by a commercial kit, but stronger resistance to ascorbate. Therefore, the new formulation was advantageous.

Multicasting based optical inverse multiplexing in elastic optical network.

Optical multicasting based inverse multiplexing (IM) is introduced in spectrum allocation of elastic optical network to resolve the spectrum fragmentation problem, where superchannels could be split and fit into several discrete spectrum blocks in the intermediate node. We experimentally demonstrate it with a 1-to-7 optical superchannel multicasting module and selecting/coupling components. Also, simulation results show that, comparing with several emerging spectrum defragmentation solutions (e.g., spectrum conversion, split spectrum), IM could reduce blocking performance significantly but without adding too much system complexity as split spectrum. On the other hand, service fairness for traffic with different granularity of these schemes is investigated for the first time and it shows that IM performs better than spectrum conversion and almost as well as split spectrum, especially for smaller size traffic under light traffic intensity.

Experimental demonstration of 400 Gb/s optical PDM-OFDM superchannel multicasting by multiple-pump FWM in HNLF.

OFDM superchannel that consists of multiple low speed individually-modulated subbands has been proposed for high speed optical transmission and flexible optical networks with multiple data rate accommodation. In this work, we investigate the feasibility of superchannel multicasting and verify it utilizing multiple-pump FWM in highly nonlinear fiber. 400 Gb/s PDM-OFDM superchannel that consists of ten subbands is successfully delivered from one superchannel to up to seven different superchannels with error free operation. Pump power and signal power are also optimized to achieve the optimal multicasting performance.

The association of tea consumption with bladder cancer risk: a meta-analysis.

The association between tea consumption and bladder cancer has been confirmed in several animal studies, but one epidemiological study in 2001 showed no association between them. In order to provide an accurate assessment of this, we conducted a meta-analysis on tea consumption and bladder cancer risk. Studies were identified by a literature search in PubMed from January 1980 to March 2012 and the reference lists of relevant studies. Random effect models were used to calculate summary relative risk estimates (RR) and their corresponding 95% confidence intervals (CI) based on high contrast to low intake values. Twenty-four publications (6 cohort studies and 18 case-control studies) based on consumption of overall tea, black tea, and green tea to bladder cancer risk were included in this analysis. For overall tea, the summary RR indicated no association between tea consumption and bladder cancer (RR= 1.09, 95%CI: 0.85-1.40). In subgroup analyses, we found a moderate increase of bladder cancer risk in smoking group (RR= 1.77, 95%CI: 1.04-3.01). In the black tea group, no statistically significant association was observed (RR= 0.84, 95%CI: 0.70-1.01). Interestingly, in the subgroup of sex, a protective effect was observed between tea consumption and bladder cancer risk in female (RR= 0.61, 95%CI: 0.38- 0.98). For green tea group, there was no relationship associated with bladder cancer risk (RR= 1.03, 95%CI: 0.82- 1.31). In conclusion, our data suggest that high overall tea intake in smokers increased the risk of bladder cancer, and high black tea intake in female may reduce the risk of bladder cancer.