Intravenous leiomyomatosis with different surgical approaches: Three case reports.

BACKGROUND: Intravenous leiomyomatosis (IVL) is a rare and complicated disease, which requires surgery by a multidisciplinary team. However, the optimal surgical approach has not been determined. CASE SUMMARY: Here we report three cases of IVL treated with different surgical approaches. All patients presented with circulation symptoms. Two patients had lower extremity edema and the other had cardiopalmus. The diagnosis of IVL was confirmed based on the imagining examinations and pathological findings. All patients underwent surgical treatment and were discharged without any complications. CONCLUSION: Preoperative examination is crucial for surgical planning and surgical approach is dependent on the patient's condition and tumor involvement.

Signal-on architecture for electrochemical aptasensors based on multiple ion channels.

In this work, we described a signal-on architecture for electrochemical aptasensors that is applicable to a wide range of aptamers. Herein, we use thrombin as the model sensing target. The signal-on aptasensor is composed of multiple ion channels embedded within a polymeric membrane, with the antithrombin aptamers chemically modified onto the inner walls of each ion channel working as the sensing element. As the thrombin concentration increased, [Ru(NH(3))(6)](3+), which was electrostatically absorbed onto the negatively charged phosphate backbones of aptamers beforehand, was displaced and pushed into the ion channels from the inner walls, leading to an increase in the current of redox cations at the working electrode surface. Compared with the traditional two-electrode design using a single ion channel sensing system, our ion channel sensing system is applied multiple times within an ordinary three-electrode system, providing such advantages as a high signal-to-noise ratio and suitability for a wide variety of redox species. The results indicate that multiple ion channel sensing provides improvements of orders of magnitude in signal sensitivity. In particular, this signal-on architecture avoids the problems of limited signal gain and "false positives". Moreover, the proposed aptasensor is simple, highly selective, stable, and applicable to real samples.

Electrochemical real-time detection of L-histidine via self-cleavage of DNAzymes.

Herein, a rapid electrochemical biosensor for L-histidine based on highly specific, L-histidine-dependent DNAzymes is described. DNA with a single, sessile ribo-adenine, self-cleaves in the presence of L-histidine, allowing a ferrocene tag to transfer electrons to the electrode. The double-stranded DNA complex was chemi-absorbed to a gold electrode via a 3' terminal thiol. The signal of this proposed sensor is linear over the range, 1 nM to 10 µM, with R=0.98775. To improve signal intensity, gold nanoparticles were anchored to a gold electrode surface which had been previously modified with self-assembled monolayers of 1,6-hexanedithiol. With gold nanoparticle modification, a lower detection limit of 0.1 pM L-histidine and a good linear relationship over the range, 0.1 pM to 50 nM were obtained. The proposed biosensor presents high specificity for L-histidine, is not affected by the presence of other amino acids, and demonstrates excellent enantio-selectivity toward L-histidine. This proposed sensor protocol offers reasonable selectivity, rapid speed, and operational convenience for real sample assays.

A cascade approach to pyridines from 2-azido-2,4-dienoates and alpha-diazocarbonyl compounds.

A one-pot synthesis of substituted pyridines via a cascade reaction of 2-azido-2,4-dienoates with alpha-diazocarbonyl compounds and triphenylphosphine is reported. The process involves a Staudinger-Meyer reaction, a Wolff rearrangement, an aza-Wittig reaction, and an electrocyclic ring-closure. The procedure is general and efficient. The substrates are readily available.

A tandem approach to isoquinolines from 2-azido-3-arylacrylates and alpha-diazocarbonyl compounds.

2-Azido-3-arylacrylates react with alpha-diazocarbonyl compounds and triphenylphosphine to furnish isoquinolines in 60-92% yields. The tandem process involves a Wolff rearrangement, an aza-Wittig reaction, and an electrocyclic ring closure. The procedure is efficient, rapid, and general, and the substrates are readily available.