ABSTRACT
OBJECTIVE: To demonstrate the feasibility of performing optical coherence tomography of the human larynx on the awake patient with a novel flexible fiberoptic delivery system. STUDY DESIGN: Prospective clinical trial. SUBJECTS AND METHODS: Imaging was performed in 17 awake patients. A flexible optical coherence tomography probe was inserted through the nose and placed in near or gentle contact with laryngeal tissues under direct endoscopic visualization. RESULTS: Images were successfully obtained from all laryngeal subsites and clearly identified laryngeal mucosal microanatomy. Several critical probe design modifications improved rotational and angular control of the distal tip while allowing linear translation of the probe and allowing more accurate apposition of the probe onto target tissues, which is critical for transnasal laryngeal imaging. CONCLUSION: This study demonstrates the feasibility of awake transnasal laryngeal optical coherence tomography and identifies key instrumentation needed to obtain useful images.
Subject(s)
Larynx , Tomography, Optical Coherence/instrumentation , Adult , Aged , Aged, 80 and over , Equipment Design , Feasibility Studies , Female , Fiber Optic Technology , Humans , Male , Middle AgedABSTRACT
BACKGROUND: Optical coherence tomography (OCT) is an evolving technology that is capable of delivering real-time, high-resolution images of tissues. The purpose of this study was to evaluate the feasibility of using OCT for detecting airway pathology in a septic animal model. METHODS: The tracheas of New Zealand white rabbits were inoculated endobronchially with various concentrations of live Streptococcus pneumoniae bacteria. After the development of pneumonia/sepsis, the animals were killed. OCT tracheal images and corresponding histologic specimens from these experimental animals were compared to control rabbit tracheas for morphologic features and quantitative tracheal mucosal thickness measurements. RESULTS: The results revealed significant airway mucosal thickening in the experimental group that was consistent with tracheal edema. Morphologic changes, including epithelial denuding and mucosal sloughing, were evident in regions of the experimental tracheas. CONCLUSION: This study suggests that OCT is a potentially valuable imaging modality that is capable of evaluating superficial airway pathology with high-resolution in vivo images. Numerous applications of OCT can be envisioned in the realm of pulmonary medicine and thoracic surgery that may substantially increase the precision and accuracy of current bronchoscopic diagnostic and surgical techniques.
Subject(s)
Pneumonia/pathology , Tomography, Optical Coherence/methods , Trachea/pathology , Animals , Disease Models, Animal , Pneumococcal Infections/complications , Pneumococcal Infections/pathology , Pneumonia/etiology , Pneumonia/microbiology , Pulmonary Edema/etiology , Pulmonary Edema/microbiology , Pulmonary Edema/pathology , Rabbits , Respiratory Mucosa/pathology , Streptococcus pneumoniae/pathogenicity , Trachea/microbiologyABSTRACT
OBJECTIVE: Hamster cheek pouches (HCP) with various degrees of 9,10-dimethyl-1,2-benzanthracene (DMBA)-induced dysplasia and malignancies were imaged with OCT/ODT in vivo and in vitro to assess the potential for three-dimensional high-resolution optical localization of airway malignancy. BACKGROUND DATA: Optical coherence tomography (OCT)/optical doppler tomography (ODT) provide potential capability for real-time in vivo high-resolution (2-20 microm) cross-sectional imaging of tissues and spatially resolved blood flow in microvasculature for pathology diagnostics. METHODS: DMBA was applied to the right side of the cheek pouch (HCP), and mineral oil (control) to the left side three times weekly for 10-18 weeks in Syrian Golden Hamsters using a standard protocol for malignancy induction. HCP were imaged in vivo with OCT/ODT as well as in vitro post-excision, using a prototype 1310-nm broadband superluminescent diode-based OCT/ODT device constructed in our laboratory. Three-dimensional images were constructed, and compared to standard and three-dimensional histology hematoxylin and eosin staining. RESULTS AND CONCLUSION: OCT imaging offered exceptional resolution of the HCP to depths of 1-2 mm and confirmed ability to detect dysplasia and malignancy. Three-dimensional OCT images were readily constructed, allowing visualization of extent and localization of tumor margins. ODT demonstrated increased vascularity in the area of neoplasia. OCT/ODT is a promising new technology for oral airway diagnostics.