Endoscopic optical coherence tomography angiography using an externally driving catheter.

Endoscopic optical coherence tomography (OCT) is an imaging modality that enables cross-sectional subsurface imaging of tubular organs and cavities. Recently, endoscopic OCT angiography (OCTA) was successfully achieved in distal scanning systems using an internal-motor-driving catheter. In conventional OCT systems using externally driving catheters, the mechanical instability in the proximal actuation causes difficulties for differentiating capillaries in tissues. In this study, OCTA in an endoscopic OCT system using an external-motor-driving catheter was proposed. Blood vessels were visualized by using a high-stability inter-A-scan scheme and the spatiotemporal singular value decomposition algorithm. It is not limited by nonuniform rotation distortion caused by the catheter and physiological motion artifacts. Results show that microvasculature in a custom-made microfluidic phantom and the submucosal capillaries in the mouse rectum are successfully visualized. Furthermore, OCTA using a catheter with a small size (outer diameter less than 1 mm) makes it possible for early diagnosis of narrow lumens, such as pancreatic and bile duct cancers.

Integrated US-OCT-NIRF Tri-Modality Endoscopic Imaging System for Pancreaticobiliary Duct Imaging.

Pancreaticobiliary carcinomas is a highly malignant gastrointestinal tumor. Most pancreaticobiliary cancers arise from epithelial proliferation within the pancreaticobiliary ducts, referred to as pancreatic intraepithelial neoplasias (PanINs). Some PanINs are benign metaplasia, while others progress to invasive duct adenocarcinoma (IDAC). However, there is no standard program to diagnose the progression from PanINs to IDAC. In this study, we present a tri-modality imaging system, which integrates ultrasound (US), optical coherence tomography (OCT), and near-infrared fluorescence (NIRF) for pancreaticobiliary duct imaging. This system can obtain OCT, US, and NIRF images in real-time with a frame rate of 30 frames per second. For the endoscopy probe with an outer diameter of 0.9 mm, the US transducer and fiber ball lens were placed back to back. In vivo experiments were performed on the rectums of Sprague-Dawley rats to demonstrate the imaging performance of US, OCT, and fluorescence angiography. An ex vivo experiment on a human pancreatic duct was performed for a more accurate assessment of the pancreaticobiliary duct. The tomography images of rat rectums and human pancreatic ducts were correlated with hematoxylin and eosin (H&E) histology to check the measurement accuracy. The integrated tri-modality system has great clinical potential in mechanism studies, early diagnosis, and prognosis evaluation of malignant pancreaticobiliary carcinomas.

Abscisic acid in soil facilitates community succession in three forests in China.

Plants release secondary metabolites into the soil that change the chemical environment around them. Exogenous abscisic acid (ABA) is an important allelochemical whose role in successional trajectories has not been examined. We hypothesized that ABA can accumulate in the soil through successional processes and have an influence on forest dynamics. To this end, we investigated the distribution of ABA in forest communities from early to late successional stages and the response of dominant species to the gradient of ABA concentrations in three types of forests from northern to southern China. Concentrations of ABA in the soils of three forest types increased from early to late successional stages. Pioneer species' litters had the lowest ABA content, and their seed germination and seedling early growth were the most sensitive to the inhibitory effect of ABA. Mid- and late-successional species had a much higher ABA content in fallen leaves than pioneer species, and their seed germination and seedling early growth were inhibited by higher concentrations of ABA than pioneers. Late-successional species showed little response to the highest ABA concentration, possibly due to their large seed size. The results suggest that ABA accumulates in the soil as community succession proceeds. Sensitivity to ABA in the early stages, associated with other characteristics, may result in pioneer species losing their advantage in competition with late-successional species in an increasingly high ABA concentration environment, and being replaced by ABA-tolerant, late-successional species.

Roots of pioneer trees in the lower sub-tropical area of Dinghushan, Guangdong, China.

Representative pioneer tree root systems in the subtropical area of South China were examined with regard to their structure, underground stratification and biomass distribution. Excavation of skeleton roots and observation of fine roots of seven species including the Euphorbiaceae, Theaceae, Melastomataceae, Lauraceae and Fagaceae families was carried out. The results showed that: (1) Pioneer tree roots in the first stage of natural succession were of two types, one characterized by taproot system with bulky plagiotropic branches; the other characterized by flat root system with several tabular roots. The late mesophilous tree roots were characterized by one obvious taproot and tactic braches roots up and down. Shrub species roots were characterized by heart fibrous root type featured both by horizontally and transversally growing branches. Root shapes varied in different dominant species at different stages of succession. (2) Roots of the different species varied in the external features-color, periderm and structure of freshly cut slash. (3) In a set of successional stages the biomass of tree roots increased linearly with the age of growth. During monsoon, the total root biomass amounted to 115.70 t/ha in the evergreen broad-leaved forest; 50.61 t/ha in needle and broad-leaved mixed forest dominated by coniferous forest; and 64.20 t/ha in broad-and needle-leaved mixed forest dominated by broad-leaved heliophytes, and are comparable to the underground biomass observed in similar tropical forests. This is the first report about roots characteristics of forest in the lower sub-tropical area of Dinghushan, Guangdong, China.