The Effect of Light Pollution on the Sea Finding Behavior of Green Turtle Hatchlings on Lanyu Island, Taiwan.

octurnal animals use their vision and acute hearing to adapt to the nighttime environment. Light pollution has become a serious problem for nocturnal animals in coastal areas, especially nesting sea turtles and sea turtle hatchlings. Hatchlings use visual clues to find the ocean. However, when the artificial light is stronger than the natural light, hatchlings become either misoriented, disoriented or both. Due to rapid tourism development on Lanyu Island, new sources of light pollution, especially streetlights, pose a serious threat to sea turtle hatchlings. In this study, we used a portable lamp constructed by Liteon Inc. on a circular area of a turtle nesting beach to see how artificial light sources could affect green turtle hatchlings' sea finding behavior. In the experiments, we tested hatchling behavior under different lamp settings (strong or weak light intensity; white or yellow light; lamp shield presence or absence) and moon visibilities (moonlit or moonless). The hatchlings' crawling tracks and locations at the end of the trials were recorded. Results showed that the light intensity had no effect on hatchling sea finding behavior. White light had a stronger impact on hatchling sea finding behavior than yellow light. When the lamp shield was installed on moonlit nights, more hatchings were able to find the sea under both white and yellow lights. Thus, it is recommended that light shields be installed on the streetlights of Lanyu Island in order to protect the sea turtle hatchlings effectively.

Disseminated Candidiasis and Candidemia Caused by Candida palmioleophila in a Green Sea Turtle (Chelonia mydas).

A sub-adult green sea turtle (Chelonia mydas) was rescued and treated for carapace and plastron shell fractures. The turtle was kept dry-docked for the first 2 months with a placement of a long-term jugular central venous catheter (CVC). Pain management, aggressive antibiotic and anthelmintic therapy, fluid therapy, force feeding, and wound debridement were provided to manage the shell fractures and control bacteremia. Human albumin was administered to treat severe hypoalbuminemia. On day 59, small budding yeasts were noted on the blood smears. Candidemia was confirmed by blood culture, as the yeasts were identified as Candida palmioleophila by the molecular multi-locus identification method. The CVC was removed, and the patient was treated with itraconazole. Although the carapace and plastron wounds had epithelized by 5.5 months after the rescue, the turtle died unexpectedly by 7.5 months. The postmortem examination revealed numerous necrogranulomas with intralesional yeasts, morphologically compatible with Candida spp., in joints, bones, brain, and lungs, suggestive of disseminated candidiasis. We describe a rare case of candidemia in the veterinary field. To our knowledge, this is the first report of candidiasis caused by C. palmioleophila in a reptile. The present results should improve veterinary medical care and, therefore, enhance the conservation of endangered sea turtle species.

Light Microscopic and Ultrastructural Characteristics of Heterophil Toxicity and Left-shifting in Green Sea Turtles (Chelonia mydas) from Taiwan.

Heterophil toxic change (TC) and left-shifting (LS) are widely used as indicators of accelerated granulopoiesis. However, the ultrastructure of heterophil TC and LS in sea turtles remain poorly understood. This study aimed to describe the ultrastructural characteristics of sea turtle TC and LS heterophils, compare the staining quality of accessible staining methods, and provide a better understanding of the clinical applications and limitations of heterophil TC and LS examinations. Blood samples were collected from 21 rescued sea turtles from January 2017 to September 2018. Morphologic (n = 22) and ultrastructural (n = 15) examination of TC and LS heterophils were performed, and the qualities of three staining methods (Wright-Giemsa stain, Diff-Quik stain and Liu's stain) were analyzed to diagnose TC and LS heterophils. In addition, the diagnostic values of TC and LS heterophils were examined. Diff-Quik stain was significantly inferior in the assessment of heterophil TC and/or LS comparing to the Wright-Giemsa stain and Liu's stain (Mann-Whitney test, P < 0.001). Microscopic examinations of heterophil TC and/or LS were comparable to transmission electron microscopy examinations (Cohen's kappa coefficient, κ = 1). The correlation between the presence of heterophil TC and/or LS and clinical inflammatory state was weak (Spearman's rank correlation coefficient, rs = 0.171, p = 0.445). In conclusion, this is the first study to describe the ultrastructural characteristics of reptile TC and LS heterophils. Wright-Giemsa stain and Liu's stain were suitable staining methods for the microscopic observations of TC and LS heterophil in sea turtles. Given the poor correlation between TC and/or LS and clinical findings, TC and LS are not a suitable diagnostic indicator of green sea turtles' inflammation status.

Using Programming Languages and Geographic Information System to Determine Spatial and Temporal Variability in a Green Turtle Foraging Population on Liuchiu Island, Taiwan.

Facial photo identification (ID) has proven to be a non-invasive method for identifying individual wild animals, and in recent years it has been effective on megafauna such as sea turtles. However, when processing hundreds of photos over a long period of time, variation in facial scale patterns makes identifying individuals complicated. This means that there is a high possibility that the individual is misidentified, which results in incorrectly determining population sizes. This study used the programming languages Python and SQL to determine green turtle foraging population size in the nearshore waters of a coral island, Liuchiu Island, from 2011 to 2017. The programs determined that the foraging population was 432 turtles, approximately 90% of which resided there one year or less and selected only one foraging site. Those that stayed for more than two years selected two foraging sites. Less than 3% stayed throughout the 7 years. The core residence area was from Beauty Cave to Vase Rock. This study found that the nearshore waters of Liuchiu Island are a temporary development/foraging site for immature green turtles. This is the first study to use Python analysis to determine a foraging sea turtle population in the field.

Twenty-three Years of Sea Turtle Stranding/ bycatch Research in Taiwan.

Coastal sea turtle stranding and bycatch are common phenomena worldwide and have received more attention in recent years. They are caused by both natural and anthropogenic factors. One thousand and seventy-two turtles were reported to be victims of these phenomena from March 1997 to November 2019 in Taiwan. Number of stranding/bycatch were variable and infrequent for the first 14 years, but increased each year after 2012 and peaked in 2019 with 217 cases. Most turtles were juveniles to subadults. All five of Taiwan's species were reported in stranding and bycatch records, and the green turtle was reported the most common. The main reported seasons lasted from winter to spring, when the weather changes dramatically. The sex ratio (female: male) ranged from 7 in the hawksbill turtle to 0.7 in the olive ridley, with an average of 2.4 for all species. Green turtles were the dominant stranded species, and more loggerhead turtles were by-caught. The hotspots were the towns of Dougou and Tochen in Yilan County, and Gongliao District in New Taipei City, located in NE coast of Taiwan respectively. Stranding was the more common of the two phenomena reported, and 80% of all stranded turtles were subadult green turtles. Eighty percent of all stranded/bycaught turtles were dead. Pond-nets were the fishing gear that accounted for the most bycatch, and captured mainly living young and subadult green turtles as well as subadult loggerhead turtles. The hotspots for bycatch were the towns of Dongou and Tochen in Yilan County. The Coast Guard and concerned citizen were the main sources of reports. This is the first study to analyze the long-term stranding/bycatch of sea turtles in Taiwan.

Geographically closed, yet so different: Contrasting long-term trends at two adjacent sea turtle nesting populations in Taiwan due to different anthropogenic effects.

Marine turtles are endangered megafauna that face both natural disturbances and anthropogenic threats. The islands of Wan-an and Lanyu support two important green turtle nesting populations in Taiwan and are separated by 250 km. Nesting activity was first documented on Wan-an Island in 1992, with 8 nesting females being documented. A further 11 nesting females were first documented on Lanyu Island in 1997. However, by 2015, the Wan-an Island population declined to only 2 nesting females, whereas the Lanyu Island population showed peaks in abundance (up to 24 nesters) every 3-5 years with no long-term decline. Additionally, the recruitment of new nesters to the Wan-an Island population decreased to 15%, whereas recruitment into the Lanyu Island population remained high (66%). The decrease of the nesting population on Wan-an Island might be due to illegal poaching on the high seas along the migratory corridor of the turtles, whereas the stable nesting population on Lanyu Island showed no evidence of such a threat. The two nesting populations use different migratory corridors to their foraging grounds, resulting in different fates of development in population trend.

Development of a Summarized Health Index (SHI) for use in predicting survival in sea turtles.

Veterinary care plays an influential role in sea turtle rehabilitation, especially in endangered species. Physiological characteristics, hematological and plasma biochemistry profiles, are useful references for clinical management in animals, especially when animals are during the convalescence period. In this study, these factors associated with sea turtle surviving were analyzed. The blood samples were collected when sea turtles remained alive, and then animals were followed up for surviving status. The results indicated that significantly negative correlation was found between buoyancy disorders (BD) and sea turtle surviving (p < 0.05). Furthermore, non-surviving sea turtles had significantly higher levels of aspartate aminotranspherase (AST), creatinine kinase (CK), creatinine and uric acid (UA) than surviving sea turtles (all p < 0.05). After further analysis by multiple logistic regression model, only factors of BD, creatinine and UA were included in the equation for calculating summarized health index (SHI) for each individual. Through evaluation by receiver operating characteristic (ROC) curve, the result indicated that the area under curve was 0.920 ± 0.037, and a cut-off SHI value of 2.5244 showed 80.0% sensitivity and 86.7% specificity in predicting survival. Therefore, the developed SHI could be a useful index to evaluate health status of sea turtles and to improve veterinary care at rehabilitation facilities.

Host-specific phenotypic plasticity of the turtle barnacle Chelonibia testudinaria: a widespread generalist rather than a specialist.

Turtle barnacles are common epibionts on marine organisms. Chelonibia testudinaria is specific on marine turtles whereas C. patula is a host generalist, but rarely found on turtles. It has been questioned why C. patula, being abundant on a variety of live substrata, is almost absent from turtles. We evaluated the genetic (mitochondrial COI, 16S and 12S rRNA, and amplified fragment length polymorphism (AFLP)) and morphological differentiation of C. testudinaia and C. patula from different hosts, to determine the mode of adaptation exhibited by Chelonibia species on different hosts. The two taxa demonstrate clear differences in shell morphology and length of 4-6(th) cirri, but very similar in arthropodal characters. Moreover, we detected no genetic differentiation in mitochondrial DNA and AFLP analyses. Outlier detection infers insignificant selection across loci investigated. Based on combined morphological and molecular evidence, we proposed that C. testudinaria and C. patula are conspecific, and the two morphs with contrasting shell morphologies and cirral length found on different host are predominantly shaped by developmental plasticity in response to environmental setting on different hosts. Chelonibia testudinaria is, thus, a successful general epibiotic fouler and the phenotypic responses postulated can increase the fitness of the animals when they attach on hosts with contrasting life-styles.

Effects of biotic and abiotic factors on the oxygen content of green sea turtle nests during embryogenesis.

Several biotic and abiotic factors can influence nest oxygen content during embryogenesis. Several of these factors were determined during each developmental stage of green sea turtle embryos on Wan-an Island, Penghu Archipelago, Taiwan. We examined oxygen content in 7 nests in 2007 and 11 in 2008. Oxygen in the adjacent sand, total and viable clutch sizes, air, sand and nest temperatures, and sand characters of each nest were also determined. Oxygen content was lower in late stages than in the early and middle stages. It was also lower in the middle layer than in the upper and bottom layers. Nest temperature showed opposite trends, reaching its maximum value in late stages of development. Nest oxygen content was influenced by fraction of viable eggs, total clutch sizes, sand temperatures, maximum nest temperature and maximum change in the nest temperature during incubation. Clutch size during embryogenesis was the most influential factor overall. However, the major influential factors were different for different developmental stages. In the first half of the incubation, the development rate was low, and the change in the nest oxygen content was influenced mainly by the clutch size. During the second half, the rapid embryonic development rate became the dominant factor, and hatchling activities caused even greater oxygen consumption during the last stage of development.