ABSTRACT
Novel coronavirus pneumonia, also known as coronavirus disease 2019 (COVID-19), is caused by sub-severe acute respiratory syndrome type 2 coronavirus (SARS-CoV-2) infection. The spike (S) protein of SARS-CoV-2 binds to angiotensin-converting enzyme 2 (ACE2) receptors widely expressed on the surface of human cells leading to life-threatening respiratory infections. A serious hazard to human health is posed by the lack of particular treatment medications for this virus infection. We advocate the creation of high-affinity antibodies using the receptor binding domain (RBD) of S protein as a specific antigenic epitope to develop a drug that can precisely target therapy COVID-19 because SARS-CoV-2 infection of the host cells is dependent on S protein binding to ACE2. Finally, we obtained high-affinity antibodies 14F4HL and 14E3HL that have high affinity with RBD and well-drug-forming properties, suitable for further humanization studies. Thus, monoclonal antibodies that neutralize the S protein were identified in our study, which may provide new insights for the development of COVID-19 therapeutic drugs.
ABSTRACT
Objective:To observe the efficacy of Xiaoxuan Zhiyun tablet in treating cervical vertigo with syndrome of phlegm and blood stasis resistance and its effect on oxidative stress and serum levels of endothelin-1(ET-1), 5-hydroxytryptamine (5-HT), and nitric oxide (NO). Method:One hundred eighteen cervical vertigo cases were selected and randomly divided into control group (59 cases) and treatment group (59 cases) by random number table. Control group was given bone setting combined with acupuncture. In addition to the therapy of control group, treatment group was given Xiaoxuan Zhiyun tablet (1 dose/day, tid). Both groups were treated for 3 weeks. Scores of cervical vertigo symptom and function assessment scale (ESCV) and vertigo disorder scale (DHI), blood velocity of vertebrobasilar artery, life quality and efficacy were compared between both groups. Serum levels of superoxide dismutase (SOD), malondialdehyde (MDA), ET-1, 5-HT and NO were detected in both groups. Result:After treatment, ESCV score of treatment group was obviously higher, while DHI score was lower than control group (PPPPPConclusion:In addition to the routine therapy, the efficacy of Xiaoxuan Zhiyun tablet in treating cervical vertigo is significant, which may be related with regulation of oxidative stress and serum levels of ET-1, 5-HT and NO.
