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1.
Int J Androl ; 30(1): 21-7, 2007 Feb.
Article in English | MEDLINE | ID: mdl-17328721

ABSTRACT

The tubuli recti (TR) are immunologically special, because the lymphocytes preferably accumulate around them during the course of T-cell dependent testicular autoimmunity in mice. This finding implies that the testicular interstitium around the TR is where autoreactive lymphocytes can gain access to autoimmunogenic germ cell antigens. In the present study, the histoarchitecture of the TR was minutely examined in normal mice. Three-dimensional analysis showed that 14-16 TR appeared to be connected to the rete testis (RT). Electron microscopical analysis revealed that the epithelial cells in the TR formed protruding cytoplasmic strings, with active endocytosis of degenerated spermatozoa, and exhibited three different morphological characteristics. Furthermore, a few macrophages were found to have penetrated into the TR. Immunohistochemical image analysis revealed that more macrophages specifically accumulated around the TR than the RT or seminiferous tubules. These findings indicate that macrophages preferentially accumulate around the TR, where contact between germ cell antigens and macrophages may occur under normal and pathological conditions.


Subject(s)
Leydig Cells/immunology , Leydig Cells/ultrastructure , Lymphocytes/immunology , Rete Testis/immunology , Rete Testis/ultrastructure , Animals , Endocytosis , Macrophages/immunology , Male , Mice , Mice, Inbred ICR , Seminiferous Epithelium/cytology , Seminiferous Epithelium/immunology , Spermatozoa/cytology , Spermatozoa/immunology
2.
Asian J Androl ; 9(2): 199-205, 2007 Mar.
Article in English | MEDLINE | ID: mdl-16855774

ABSTRACT

AIM: To determine the biochemical effect of di-(2-ethylhexyl) phthalate (DEHP) on testes, liver, kidneys and pancreas on day 10 in the process of degeneration of the seminiferous epithelium. METHODS: Diets containing 2% DEHP were given to male Crlj:CD1(ICR) mice for 10 days. The dose of DEHP was 0.90 +/- 0.52 mg/mouse/day. Their testes, livers, kidneys and pancreata were examined for detection of mono-(2-ethylhexyl) phthalate (MEHP), nitrogen oxides (NOx) produced by peroxidation of nitric oxide (NO) with free radicals, and lipid peroxidation induced by the chain reaction of free radicals. RESULTS: Histological observation and serum analysis showed the presence of severe spermatogenic disturbance, Leydig cell dysfunction, liver dysfunction and dehydration. Unexpectedly, the concentration of MEHP in the testes was extremely low compared with that in the liver. However, the concentration of the NOx in the testes was as high as the hepatic concentration. Furthermore, free radical-induced lipid peroxidation was histochemically detected in the testes but not in the liver. CONCLUSION: The results indicate that DEHP-induced aspermatogenesis is caused by the high sensitivity of the testicular tissues to MEHP rather than the specific accumulation or uptake of circulating MEHP into the testes.


Subject(s)
Diethylhexyl Phthalate/pharmacology , Kidney/metabolism , Liver/metabolism , Pancreas/metabolism , Testis/metabolism , Animals , Body Weight/drug effects , Copper/metabolism , Diethylhexyl Phthalate/analogs & derivatives , Diethylhexyl Phthalate/metabolism , Iron/metabolism , Kidney/drug effects , Lipid Peroxidation/drug effects , Liver/drug effects , Male , Mice , Mice, Inbred ICR , Nitrogen Oxides/metabolism , Pancreas/drug effects , Spermatogenesis/drug effects , Testis/drug effects , Testosterone/blood , Zinc/metabolism
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