Investigating the addition of Fe for improving contaminant removal and regulating microbes in a simulated coastal wetland.

Contaminants from wastewater of aquaculture are increasing the risks of red tides in coastal areas. Such types of contaminants are difficult to remove by using conventional biological and ecological treatment methods because of the relatively low C/N ratios and the high salinity in coastal water ambience. Fe is considered a key element in natural chemical cycling and promotes the growth of animals and plants as well. The cycling of Fe ion combined with carbon, nitrogen, and phosphorus stimulates bacterial growth. As a result, it acts as a microbial carbon pump in coastal areas, such as natural wetlands, which have been activated and adapted to be salinity resistant and insufficient energy supply. Along these lines, in this work, constructed wetlands (CWs) with high ecological benefits and low cost of maintenance were used to treat aquaculture wastewater. The impact of Fe ion recycling on multiple contaminants was also systematically investigated. The two types of Fe dosage were pure ferrous ions and a mixture of iron powder and ferrous ions. After the application of a 3-day treatment, the dosage of iron powder/ferric ions (1:1 m/m) at a concentration of 15 mg L-1 showed a better effect, where the total nitrogen, total phosphorus, and chemical oxygen demand removal rates were increased by 2.95%, 2.16%, and 9.76%, respectively. From the microbial analysis, it was indicated that Fe ion affected the abundance and functions of the microbial communities in the CWs. The significant enrichment of Proteobacteria promoted the removal of multiple contaminants under saline stress and fixed carbon, and affected the whole microbe distribution and diversity in CWs. The implementation of such an environmentally friendly and economical approach arises as a promising candidate for the efficient removal of multiple contaminants from aquacultural wastewater in coastal zones.

Augmentation of saline wastewater treatment via functional enrichment of bacteria and optimized distribution in constructed wetlands combined with slag-sponges at different temperatures.

China's aquatic environment continues to face several difficulties. Ecological constructed wetland systems (CWs) can be used to treat polluted saline water to alleviate water shortages regionally and globally. However, the performance is limited by low temperatures. To expand the use of CWs, we introduced a slag-sponge, a flaky material derived from alkaline waste slag, to create a newly constructed wetland system that can operate at both low and high temperatures. We evaluated its effectiveness by placing it at different heights in our devices. The results showed that the treatment was effective for saline wastewater with multiple contaminants. The efficiency was 20% higher than that of traditional CWs. Slag-sponges were found to carry pore structures and exhibit thermal insulation, which led to the enrichment of functional microbial communities (Chryseobacterium and Exiguerium) at low temperatures according to the microbial species analysis. The enhanced CWs offer another option for the treatment of polluted saline water in the environment and provide promising strategies for the utilization of waste slag.

Excellent Pd-Loaded Magnetic Nanocatalyst on Multicarboxyl and Boronic Acid Biligands.

An effective palladium nanocatalyst (Fe3O4@SiO2-FPBA-DTPA-Pd) was proposed and prepared, which was immobilized on magnetic silica with ethylenediamine pentaacetic acid and formylphenylboronic acid as biligands. A series of characterizations showed that Fe3O4@SiO2-FPBA-DTPA-Pd was 5-15 nm and contained 1.44 mmol/g Pd2+/Pd0. It was stable below 232.7 °C, and its saturation magnetization value was 21.17 emu/g which was easily recycled by a magnet. Its catalytic ability was evaluated through 7 Suzuki reactions and 15 Heck reactions. Results showed that the yields of 14 reactions catalyzed by Fe3O4@SiO2-FPBA-DTPA-Pd were more than 90%, while were better than those of the other two immobilized Pd catalysts on a single diethyltriamine pentaacetic acid (DTPA) group or boronic acid group. Moreover, Fe3O4@SiO2-FPBA-DTPA-Pd showed good reusability in both Suzuki and Heck reactions. In two model Suzuki and Heck reactions, after seven cycles, its yields were still above 95% without significant loss, which exceeded those of many reported catalysts; therefore, it has great potential in future large-scale industrial production.

Evaluating the opportunities for mainstream P-recovery in anaerobic/anoxic/aerobic systems.

Mainstream P-recovery can help wastewater treatment plants (WWTPs) to effectively maintain good enhanced biological phosphorus removal (EBPR) while helping to recover P. In this study, a pilot-scale anaerobic-anoxic-aerobic (A2O) process was operated for simultaneous COD/N/P removal and P-recovery under different operational conditions. The operation with conventional extraction of waste activated sludge (WAS) from the aerobic reactor was compared to the mainstream P-recovery strategy of WAS extraction from the anaerobic reactor. Successful nutrient removal was obtained for both scenarios, but the anaerobic WAS extraction results improved polyphosphate accumulating organisms (PAOs) activity by increasing almost 27 % P concentration in the anaerobic reactor. WAS fermentation was also evaluated, showing that anaerobic WAS required only 3 days to reach a high P concentration, while the aerobic WAS fermentation required up to 7 days. The fermentation process increased the amount of soluble P available for precipitation from 24.4 % up to 51.6 % in the fermented anaerobic WAS scenario. Results obtained by precipitation modelling of these streams showed the limitations for struvite precipitation due to Ca2+ interference and Mg2+ and NH4+ as limiting species. The optimum precipitation scenario showed that P-recovery could reach up to 51 % of the input P, being 90 % struvite.

Phenylboronic Acid and Amino Bifunctional Modified Adsorbent for Quickly Separating Phenolic Acids from Crude Extract of Clerodendranthus spicatus and Evaluation of Their Antioxidant and Hypoglycemic Activities.

A novel phenylboronic acid and amino bifunctional modified silica gel (SiO2-NH2-FPBA) was prepared, which was 30-80 µm, had a pore size of 8.69 nm, a specific surface area of 206.89 m2/g, was stable at low temperature, and contained 0.4793 mmol/g of the phenylboronic acid group and 1.6377 mmol/g of the amino group. It was used to develop a rapid separation method for phenolic acids. The results showed that it could adsorb 93.64 mg/g caffeic acid, 89.35 mg/g protocatechuic acid and 79.66 mg/g gallic acid. The adsorption process was consistent with the pseudo-second-order model (R2 > 0.99), and fitted the Langmuir isotherm model well (R2 > 0.99). CH3COOH could effectively desorb phenolic acids (>90%) and did not destroy their structures. When SiO2-NH2-FPBA was added to crude extract of Clerodendranthus spicatus, 93.24% of the phenolic acids could be captured, and twenty-two kinds of phenolic acids were identified by Q Exactive HF LC-MS. Furthermore, the isolated phenolic acids from Clerodendranthus spicatus possessed great DPPH, ABTS, and hydroxyl radicals scavenging activities and ferric reducing power. They also demonstrated effective inhibition of α-amylase and α-glucosidase activities (IC50 = 110.63 ± 3.67 µg/mL and 64.76 ± 0.30 µg/mL, respectively). The findings indicate that SiO2-NH2-FPBA has significant potential in practical applications of separating active constituents from natural resources.

Enhancement of chiral drugs separation by a novel adjustable gravity mediated capillary electrophoresis combined with sulfonic propyl ether ß-CD polymer.

A water-soluble negative sulfonic propyl ether ß-CD polymer (SPE-ß-CDP) to be used as chiral selector in capillary electrophoresis (CE) was polymerized. The sulfonic substitution degree of each ß-CD in SPE-ß-CDP was statistically homogenized. The only one negative peak in electrophoretogram with indirect ultraviolate method proved its uniformity of electrophoretic behavior. There were 7.12 sulfonic substitution in ß-CD unit and 164 µmole ß-CD units in each gram of SPE-ß-CDP, which corresponded a molecular weight of 7000 or more. Compared with monomer, SPE-ß-CDP was lower effect on electrical current of CE, indicating a high concentration of SPE-ß-CDP could be added. Its separation ability was verified by 12 chiral drugs. SPE-ß-CDP also showed advantages of good water solubility, easy preparation and recovery to reduce the overall cost. However, five of 12 chiral drugs were hardly to be fully separated which was normal for any kind of chiral selector. A newly adjustable gravity mediated capillary electrophoresis (AGM-CE) technology was proposed and combined with SPE-ß-CDP to enhance the chiral separation efficiencies of propranolol, salbutamol, omeprazole, ofloxacin and phenoxybenzamine which were markedly improved to 3.02, 1.17, 7.63, 4.14, and 2.81, respectively. Furthermore, its gradient mode (AGMg-CE) was also used to improve resolution through utilizing the zero mobility point, at which the effective apparent mobility of one racemate was zero. Resolutions of five chiral drugs were significantly improved, especially resolution of carvedilol changed from 0.43 to 1.0. These indicated SPE-ß-CDP as chiral selector, AGM-CE and AGMg-CE as new CE technologies had a great potential in chiral separation.

The HNF4A-CHPF pathway promotes proliferation and invasion through interactions with MAD1L1 in glioma.

Chondroitin polymerizing factor (CHPF) is an important glycosyltransferases that participates in the biosynthesis of chondroitin sulfate (CS). Our previous study showed that silencing CHPF expression inhibited glioma cell proliferation in vitro, but the molecular mechanisms by which CHPF contributes to development of glioma have not been characterized. In this study, we found that CHPF was up-regulated in glioma tissues and was positively correlated with malignant clinical pathological characteristics of patients with glioma. Silencing CHPF expression inhibited proliferation, colony formation, migration, and cell cycle of glioma cells. Moreover, silencing CHPF suppressed glioma malignance in vivo. Immunoprecipitation, co-immunoprecipitation, GST pulldown, and liquid chromatography-mass spectrometry (LC-MS/MS) assays were used to verify the interaction between CHPF and Mitotic arrest deficient 1-like 1 (MAD1L1). In addition, Chromatin Immunoprecipitation (ChIP)-PCR analysis showed that HNF4A bound to the CHPF promoter region, which indicated that the transcription factor hepatocyte nuclear factor 4A (HNF4A) could regulate the expression of CHPF in glioma cells.

Preparation of a phenylboronic acid and aldehyde bi-functional group modified silica absorbent and applications in removing Cr(vi) and reducing to Cr(iii).

Cr(vi) is a great threat to the ecological environment and human health, so it is urgent to remove Cr(vi) from the environment. In this study, a novel silica gel adsorbent SiO2-CHO-APBA containing phenylboronic acids and aldehyde groups was prepared, evaluated and applied for removing Cr(vi) from water and soil samples. The adsorption conditions including pH, adsorbent dosage, initial concentration of Cr(vi), temperature and time were optimized. Its ability in removing Cr(vi) was investigated and compared with three other common adsorbents, SiO2-NH2, SiO2-SH and SiO2-EDTA. Data showed SiO2-CHO-APBA had the highest adsorption capacity of 58.14 mg g-1 at pH 2 and could reach adsorption equilibrium in about 3 h. When 50 mg SiO2-CHO-APBA was added in 20 mL of 50 mg L-1 Cr(vi) solution, more than 97% of Cr(vi) was removed. A mechanism study revealed that a cooperative interaction of both the aldehyde and boronic acid groups is attributed to Cr(vi) removal. The reducing function was gradually weakened with the consumption of the aldehyde group, which was oxidized to a carboxyl group by Cr(vi). This SiO2-CHO-APBA adsorbent was successfully used for the removal of Cr(vi) from soil samples with satisfactory results which indicates a good potential in agriculture and other fields.

Elbow Kinematics and Function Following Treatment with Open Arthrolysis and Hinged External Fixator.

OBJECTIVE: Open arthrolysis (OA) combined with hinged external fixator (HEF) is a promising surgical option for patients with elbow stiffness. This study aimed to investigate elbow kinematics and function following a combined treatment with OA and HEF in elbow stiffness cases. METHODS: Patients treated with OA with or without HEF due to elbow stiffness were recruited between August 2017 and July 2019. Elbow flexion-extension motion and function (Mayo elbow performance scores, MEPS) were recorded and compared between patients with and without HEF during a 1-year follow-up period. Additionally, those with HEF were assessed by dual fluoroscopy at week 6 postoperatively. Flexion-extension and varus-valgus motions, as well as ligament insertion distances of the anterior medial collateral ligament (AMCL) and lateral ulnar collateral ligament (LUCL), were compared between the surgical and intact sides. RESULTS: This study included 42 patients, of which 12 with HEF demonstrated a similar flexion-extension angle and range of motion (ROM) and MEPS as the other patients. In patients with HEF, the surgical elbows showed limitations in flexion-extension (maximal flexion, 120.5° ± 5.3° vs 140.4° ± 6.8°; maximal extension, 13.1° ± 6.0° vs 6.4° ± 3.0°; ROM, 107.4° ± 9.9° vs 134.0° ± 6.8°; all Ps < 0.01) compared with the contralateral sides. During elbow flexion, a gradual valgus-to-varus transition of the ulna, increase in the AMCL insertion distance, and steady change in the LUCL insertion distance were observed, with no significant differences between the bilateral sides. CONCLUSIONS: Patients treated with OA and HEF demonstrated similar elbow flexion-extension motion and function to those treated with OA alone. Although the use of HEF could not restore an intact flexion-extension ROM and might result in some minor but not significant changes in kinematics, it contributed to clinical outcomes comparable to that of the treatment with OA alone.

Effects of two plant species combined with slag-sponges on the treatment performance of contaminated saline water in constructed wetland.

Constructed wetland (CW), an ecological water treatment system, can purify and repair the damaged saline water body in an open watershed, but its repairing function is limited at low temperature under salt stress. In this study, two different plant species with slag-sponge layer were operated to enhance the purification effect of CW on the damaged saline water body. The results showed that the combination of Scirpus mariqueter and slag-sponges in CW had a better purification effect especially under the condition of salinity of 10‰ (S = 10) with a respective removal efficiency of 91.04% of total nitrogen, 80.07% of total phosphorus, and 93.02% of COD in high temperature (25 ~ 35 °C). Furthermore, ecological traits (enzyme activity and amino acids) of plants, the abundance and distribution of functional microorganisms on the surface of slag-sponges, and the microbial state on the substrate surface of the denitrifying zone of CW were analyzed to explain how exactly the combinations worked. It was found that the enrichment of functional microorganisms in slag-sponge and the anaerobic zone of plants have improved the nitrogen and phosphorus removal. Plants maintained high enzyme activities and the ability to synthesize key amino acids under salt stress to ensure the growth and reproduction of plants and achieve the assimilation function. Scirpus mariqueter combined with slag-sponges in CW effectively improved the purification effect of damaged saline water, indicating that it is an ecological and green saline water treatment way.

Ginseng-containing traditional medicine preparations in combination with fluoropyrimidine-based chemotherapy for advanced gastric cancer: A systematic review and meta-analysis.

BACKGROUND: Ginseng-containing traditional medicine preparations (G-TMPs) in combination with fluoropyrimidine-based chemotherapy (FBC) are well-known treatments for advanced gastric cancer (AGC), with a superior efficacy to FBC alone. However, evidence regarding their efficacy remains limited. The purpose of this meta-analysis is to evaluate the efficacy and safety of G-TMPs in combination with FBC for the treatment of AGC. METHODS: Eight electronic databases were searched for randomized controlled trials (RCTs) using G-TMPs with FBC for the treatment of AGC. The primary outcome included the tumor response, while the secondary outcomes included the quality of life (QoL), proportions of peripheral blood lymphocytes, adverse drug reactions (ADRs), and levels of cancer biomarkers. The quality of evidence for each outcome was assessed using GRADE profilers. RESULTS: A total of 1,960 participants were involved in the 26 RCTs included. Patients treated with FBC plus G-TMPs had better objective response (risk ratio [RR] = 1.23, 95% confidence interval [CI]: 1.13 to 1.35, p < 0.00001) and disease control (RR = 1.13, 95% CI: 1.08 to 1.19, p < 0.00001) rates than those treated with FBC alone. Additionally, the combination group had a better QoL, higher proportions of CD3+ T cells, CD4+ T cells, and natural killer cells, as well as a higher CD4+/CD8+ T-cell ratio. Furthermore, lower levels of CA19-9, CA72-4, and CEA were confirmed in the combination treatment group. In addition, G-TMPs reduced the incidence of ADRs during chemotherapy. CONCLUSION: In combination with FBC, G-TMPs can potentially enhance efficacy, reduce ADRs, and improve prognosis for patients with AGC. However, high-quality randomized studies remain warranted. SYSTEMATIC REVIEW REGISTRATION: PROSPERO Number: CRD42021264938.

Retrospective Clinical Study on Integrated Chinese and Western Medicine in Treatment of Limited-Stage Small Cell Lung Cancer.

OBJECTIVE: To investigate the efficacy of integrated Chinese and Western medicine extending the progression-free survival (PFS) and overall survival (OS) of limited-stage small cell lung cancer (LS-SCLC) patients after the first-line chemoradiotherapy. METHODS: The data of 67 LS-SCLC patients who received combined treatment of CM and Western medicine (WM) between January 2013 and May 2020 at the outpatient clinic of Guang'anmen Hospital were retrospectively analyzed. Thirty-six LS-SCLC patients who received only WM treatment was used as the WM control group. The medical data of the two groups were statistically analyzed. Survival analysis was performed using the product-limit method (Kaplan-Meier analysis). The median OS and PFS were calculated, and survival curves were compared by the Log rank test. The cumulative survival rates at 1, 2, and 5 years were estimated by the life table analysis. Stratified survival analysis was performed between patients with different CM administration time. RESULTS: The median PFS in the CM and WM combination treatment group and the WM group were 19 months (95% CI: 12.357-25.643) vs. 9 months (95% CI: 5.957-12.043), HR=0.43 (95% CI: 0.27-0.69, P<0.001), respectively. The median OS in the CM and WM combination group and the WM group were 34 months (95% CI could not be calculated) vs. 18.63 months (95% CI: 16.425-20.835), HR=0.40 (95% CI: 0.24-0.66, P<0.001), respectively. Similar results were obtained in the further stratified analysis of whether the duration of CM administration exceeded 18 and 24 months (P<0.001). CONCLUSION: The combination treatment of CM and WM with continuing oral administration of CM treatment after the first-line chemoradiotherapy for LS-SCLC patients produced better prognosis, lower risks of progression, and longer survival than the WM treatment alone. (Registration No. ChiCTR2200056616).

An Aggregation-Induced Emission Nanosensor for Real-Time Chemiluminescent Sensing of Light-Independent Intracellular Singlet Oxygen.

Characterizing the transient ultratrace light-independent intracellular singlet oxygen (1O2), which plays a vital role in multiple biological processes in living organisms, brings about tremendous help for understanding the nature of 1O2-mediated or related bioevents. Nevertheless, an approach to detect the light-independent intracellular 1O2 is hard to find. Herein, we developed a chemiluminescent nanosensor by compacting a great number of TPE-N(Ph)-DBT-PH molecules in one nanostructure via autoaggregation. Taking advantage of the aggregation-induced emission property, this TPE-N(Ph)-DBT-PH nanosensor is highly fluorescent and promises a bright red-light CL and the convenience of mapping in vivo sensor distribution. Experiments demonstrate the nanosensor's unprecedented selectivity toward 1O2 against other reactive oxygen species. The 3.7 nmol L-1 limit of detection renders this nanosensor with the best-known sensitivity of 1O2 chemical sensors. Meanwhile, fluorescence confocal microscope imaging results suggest that our nanosensor simultaneously targets mitochondria and lysosomes in RAW 264.7 cells via the energy-dependent endocytosis pathway, thereby implying an attractive potential for the detection of intracellular 1O2. Such a potential is demonstrated by detecting 1O2 in RAW 264.7 cells during a lipopolysaccharide and phorbol myristate acetate stimulated respiration burst. This study represents the first approach to detect light-independent intracellular 1O2 during cell bioregulation. Thus, our nanosensor provides an effective tool for investigating the 1O2-related bioprocesses and pathological processes.

Advances in microbial electrochemistry-enhanced constructed wetlands.

Constructed wetland (CW) is an effective ecological technology to treat water pollution and has the significant advantages of high impact resistance, simple construction process, and low maintenance cost. However, under extreme conditions such as low temperature, high salt concentration, and multiple types of pollutants, some bottlenecks exist, including the difficulty in improving operating efficiency and the low pollutant removal rate. Microbial electrochemical technology is an emerging clean energy technology and has the similar structure and pollutant removal mechanism to CW. Microbial electrochemistry combined with CW can improve the overall removal effect of pollutants in wetlands. This review summarizes characterization methods of microbial electrochemistry-enhanced constructed wetland systems, construction methods of different composite systems, mechanisms of single and composite systems, and removal effects of composite systems on different pollutants in water bodies. Based on the shortcomings of existing studies, the potential breakthroughs in microbial electrochemistry-enhanced constructed wetlands are proposed for developing the optimization solution of constructed wetlands.

DNA methylation regulator-mediated modification patterns and tumor microenvironment characterization in glioma.

Growing evidences indicate DNA methylation plays a crucial regulatory role in inflammation, innate immunity, and immunotherapy. However, the overall landscape of various DNA methylation regulatory genes and their relationship with the infiltration of immune cells into the tumor microenvironment (TME) as well as the response to immunotherapy in gliomas is still not clear. Therefore, we comprehensively analyzed the correlation between DNA methylation regulator patterns, infiltration of immune cell-types, and tumor immune response status in gather glioma cohorts. Furthermore, we calculated the DNA methylation score (DMS) for individual glioma samples, then evaluated the relationship between DMS, clinicopathological characteristics, and overall survival (OS) in patients with gliomas. Our results showed three distinct DNA methylation regulator patterns among the glioma patients which correlated with three distinct tumor immune response phenotypes, namely, immune-inflamed, immune-excluded, and immune desert. We then calculated DMS for individual glioma samples based on the expression of DNA methylation-related gene clusters. Furthermore, DMS, tumor mutation burden (TMB), programmed death 1 (PD-1) expression, immune cell infiltration status in the TME, and Tumor Immune Dysfunction and Exclusion (TIDE) scores were associated with survival outcomes and clinical responses to immune checkpoint blockade therapy. We also validated the predictive value of DMS in two independent immunotherapy cohorts. In conclusion, our results demonstrated that three DNA methylation regulator patterns that correlated with three tumor immune response phenotypes. Moreover, we demonstrated that DMS was an independent predictive biomarker that correlated with survival outcomes of glioma patients and their responses to immunotherapy therapeutic regimens.

UGT1A1 Allele Test Not Only Minimizes the Toxicity But Also Maximizes the Therapeutic Effect of Irinotecan in the Treatment of Colorectal Cancer: A Narrative Review.

Background: Irinotecan is a first-line agent in the systematic treatment of colorectal cancer (CRC). Adjusting the dose of irinotecan according to the uridine diphosphate glucuronosyltransferase (UGT) 1A1 genotype reflects the principle of individualized and precision medicine, and may improve the chemotherapy response and survival of CRC. Methods: To summarize the feasibility, efficacy and safety of high dose irinotecan in CRC patients with UGT1A1 wild-type or heterozygous alleles, PubMed, EMBASE, MEDLINE and the Cochrane Central Register of Controlled Trials online databases were searched from the date of creation to October 22, 2021. Results: A total of 1,186 related literatures were searched, and 14 studies were included for review according to the inclusion criteria. The results indicated that the maximum tolerated dose of irinotecan in CRC patients with UGT1A1 wild-type or heterozygous variant was significantly higher than the conventional recommended dose. Chemotherapy based on high dose irinotecan improved the clinical efficacy in mCRC patients with UGT1A1*28 wild-type and heterozygous variant, and the toxicity was tolerated, as reflected in most studies. Conclusions: We are optimistic about the application of high dose irinotecan for mCRC patients with UGT1A1*28 wild-type or heterozygous variant, which will provide a relatively clear direction for future research and certain norms for clinical practice.

Clinical Efficacy and Safety of Traditional Medicine Preparations Combined With Chemotherapy for Advanced Pancreatic Cancer: A Systematic Review and Meta-Analysis.

Background: Traditional medicine preparations (TMPs) combined with chemotherapy is widely used for patients with advanced pancreatic cancer (APC); however, its efficacy and safety are still unclear. The purpose of this meta-analysis was to evaluate the clinical efficacy and safety of TMPs combined with chemotherapy for the treatment of APC. Methods: A systematic search of eight electronic databases for randomized controlled trials (RCTs) was conducted from inception to October 15, 2021. Tumor response was identified as primary outcome, whereas quality of life (QoL), cancer biomarkers, and adverse drug reactions (ADRs) were identified as secondary outcomes. Quality of the evidence for each outcome was evaluated by GRADE profiler. Results: In total, 31 RCTs involving 1,989 individuals were included. This meta-analysis showed that TMPs combined with chemotherapy significantly improved the objective response rate (ORR) (RR=1.64, 95% CI [1.43 to 1.88], p <0.00001), disease control rate (DCR) (RR=1.29, 95% CI [1.21 to 1.38], p <0.00001), and QoL (continuous data: SMD=0.81, 95% CI [0.44 to 1.18], p <0.0001, dichotomous data: RR=1.44, 95% CI [1.22 to 1.70], p<0.0001), compared to those with chemotherapy alone. In addition, the combined treatment group also had lower levels of CA19-9 (SMD=-0.46, 95% CI [-0.90 to -0.02], p=0.04) and CEA (SMD=-0.55, 95% CI [-0.93 to -0.17], p=0.004). Moreover, TMPs reduced the ADRs during chemotherapy. Conclusion: This systematic review suggests that TMPs combined with chemotherapy might be a potential option to enhance therapeutic effects and reduce ADRs during the treatment of APC. However, more high-quality randomized controlled trials with more participants are needed. Systematic Review Registration: https://www.crd.york.ac.uk/prospero/display_record.php?RecordID=209825, identifier PROSPERO Number: CRD42021264938.

Contact toxicity and repellence of essential oil from Senecio scandens and its major components against three stored product insects.

The objective of this work was to investigate the chemical compositions of the essential oil (EO) extracted from Senecio scandens by hydrodistillation and their insecticidal activities against Tribolium castaneum, Lasioderma serricorne and Liposceis bostrychophila. The chemical profile of the EO were analyzed by gas chromatography-mass spectrometry (GC-MS), and 20 compounds were identified which accounted for 88.03% of the total EO. Five major compounds identified in the EO were assayed against the three stored product insects. The EO showed strong contact toxicity to T. castaneum (LD50 = 18.01 µg/adult), L. serricorne (LD50 = 20.11 µg/adult) and L. bostrychophila (LD50 = 72.14 µg/cm2). Among all compounds, geraniol showed the contact toxicity against L. serricorne and L. bostrychophila with LD50 values of 15.82 µg/adult and 26.64 µg/cm2. The EO and its five chemical compounds also exhibited different level of potential repellence to the three stored product insects.

A Novel Constructed Wetland Combined with Microbial Desalination Cells and its Application.

Wastewater recycling can alleviate the shortage of water resources. Saline water is seldom treated with biological processes, and its recycling rate is low. Constructed wetland (CW) is a safe, economical, and ecological water treatment method. However, the saline water treatment performance of CW is not good. Microbial desalination cells (MDC) utilizing a bioelectrochemical approach achieve functions of desalination and power generation. In this study, MDC was used to strengthen CW to form a composite system, MDC-CW. Through optimization of design parameters, MDC-CW was applied in the treatment of salt-containing water. The average total nitrogen removal rate in MDC-CW-P1 reached 87.33% and the average COD removal rate was 92.79%. The average desalination rate of MDC-CW-P1 was 55.78% and the average voltage of MDC-CW-P1 reached 0.40 mV. Planting Canna indica in the MDC-CW was conducive to the functions of desalination and power generation. The above results were also verified by the microbial analysis results of gravels in the substrate, plant rhizosphere, and electrodes. In addition, the decontamination of the device mainly depended on the function of the bacteria commonly used in water treatment, such as Proteobacteria and Bacteroidetes, whereas the generation of power depended on the function of Geobacter. Salt ions moved spontaneously to the cathode and anode under the influence of current generation so that the desalination function was realized under the selective isolation function of exchange membranes. The device design and laboratory applications of MDC-CW experimentally achieved the electrochemical function and broadened the treatment scale of CW.

Efficacy and Safety of Astragalus-Containing Traditional Chinese Medicine Combined With Platinum-Based Chemotherapy in Advanced Gastric Cancer: A Systematic Review and Meta-Analysis.

BACKGROUND: Astragalus-containing traditional Chinese medicine (TCM) is widely used as adjunctive treatment to platinum-based chemotherapy (PBC) in patients with advanced gastric cancer (AGC) in China. However, evidence regarding its efficacy remains limited. This study aimed to evaluate the efficacy and safety of Astragalus-containing TCM combined with PBC in AGC treatment. METHODS: We searched for literature (up to July 19, 2020) in eight electronic databases. The included studies were reviewed by two researchers. The main outcomes were the objective response rate (ORR), disease control rate (DCR), survival rate, quality of life (QOL), adverse drug reactions (ADRs), and peripheral blood lymphocyte levels. The effect estimate of interest was the risk ratio (RR) or mean difference (MD) with 95% confidence intervals (CIs). Trial sequential analysis (TSA) was used to detect the robustness of the primary outcome and to calculate the required information size (RIS). Certainty of the evidence was assessed using the GRADE profiler. RESULTS: Results based on available literature showed that, compared with patients treated with PBC alone, those treated with Astragalus-containing TCM had a better ORR (RR: 1.24, 95% CI: 1.15-1.34, P < 0.00001), DCR (RR: 1.10, 95% CI: 1.06-1.14, P < 0.00001), 1-year survival rate (RR: 1.41, 95% CI: 1.09-1.82, P = 0.009), 2-year survival rate (RR: 3.13, 95% CI: 1.80-5.46, P < 0.0001), and QOL (RR: 2.03, 95% CI: 1.70-2.43, P < 0.00001 and MD: 12.39, 95% CI: 5.48-19.30, P = 0.0004); higher proportions of CD3+ T cells and CD3+ CD4+ T cells; higher ratio of CD4+/CD8+ T cells; nature killer cells; and lower incidence of ADRs. Subgroup analysis showed that both oral and injection administration of Astragalus-containing TCM increased tumor response. Whether treatment duration was ≥8 weeks or <8 weeks, Astragalus-containing TCM could increase tumor response in AGC patients. Furthermore, Astragalus-containing TCM combined with oxaliplatin-based chemotherapy could increase the ORR and DCR; when with cisplatin, it could only increase the ORR. CONCLUSION: Current low to moderate evidence revealed that Astragalus-containing TCM combined with PBC had better efficacy and less side effects in the treatment of AGC; however, more high-quality randomized studies are warranted. SYSTEMATIC REVIEW REGISTRATION: PROSPERO, identifier CRD42020203486.