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1.
Mater Sci Eng C Mater Biol Appl ; 119: 111635, 2021 Feb.
Article in English | MEDLINE | ID: mdl-33321673

ABSTRACT

ZnO eugenol-based materials are widely used for restoration of caries cavity, apical retrograde filling and root canal sealer. Their effects on apical bone healing await investigation. The toxic mechanisms of ZnO particles and nanoparticles to MG-63 osteoblastic cells were studied. We found the different morphology and size of various particles as observed by scanning electron microscope. Particles of Canals and Roth801 were larger than ZnO-205532 microparticles and ZnO-677450 nanoparticles. Four ZnO particles showed cytotoxicity (>25 µg/ml) as analyzed by MTT. Transmission electron microscope found intracellular vacuoles with particle content. Exposure to ZnO particles induced ROS production and cell cycle arrest as studied by DCF and propidium iodide flow cytometry. ZnO particles activated ATM, ATR, Chk1, Chk2, γ-H2AX, ERK and p38 phosphorylation as detected by immunofluorescent staining and western blotting. The protein expression of cdc2, cyclin B1 and cdc25C were decreased, whereas GADD45α and hemeoxygenase-1 (HO-1) were stimulated. ZnO particles' cytotoxicity to MG63 cells was prevented by N-acetylcysteine (NAC), but not CGK733, AZD7762, U0126 and SB203580. ZnO showed little effect on IL-8 and sICAM-1 secretion. These results indicated that ZnO particles are toxic to osteoblasts. ZnO particles' toxicity were related to ROS, and DNA damage responses, checkpoint kinases, cell cycle arrest, ERK and p38 signaling, but not IL-8 and ICAM-1. These results were useful for materials' development and promote apical healing. Dentists should avoid of extruding ZnO-based sealers excessively over root apex and prevent residual ZnO-based retrograde filling materials in apical area during endodontic practice.


Subject(s)
Nanoparticles , Zinc Oxide , Osteoblasts , Phosphorylation , Signal Transduction
2.
Int J Mol Sci ; 21(21)2020 Oct 30.
Article in English | MEDLINE | ID: mdl-33143101

ABSTRACT

Betel quid (BQ) chewing increased the risk of oral cancer and oral submucous fibrosis (OSMF), an oral premalignant disorder (OPMD) with malignant transformation potential. BQ components such as areca nut (AN), trauma by coarse AN fiber, catechin, copper, alkaloids, stimulated reactive oxygen species (ROS), inflammation and cytotoxicity are suggested to be the contributing factors. They may induce tissue inflammation, proliferation of fibroblasts and collagen deposition, myofibroblast differentiation and contraction, collagen cross-links and inhibit collagen phagocytosis, finally leading to the development of OSMF and oral cancer. These events are mediated by BQ components-induced changes of extracellular matrix (ECM) turnover via regulation of TGF-ß1, plasminogen activator inhibitor-1 (PAI-1), cystatin, lysyl oxidase (LOX) and tissue inhibitors of metalloproteinases (TIMPs) and metalloproteinases (MMPs). Genetic susceptibility is also involved in these disease processes. Further understanding the molecular mechanisms of BQ-induced OSMF and oral cancer can be helpful for future disease prevention and treatment.


Subject(s)
Areca/adverse effects , Extracellular Matrix Proteins/metabolism , Extracellular Matrix/metabolism , Genetic Predisposition to Disease , Oral Submucous Fibrosis/pathology , Humans , Oral Submucous Fibrosis/etiology , Oral Submucous Fibrosis/metabolism
3.
J Formos Med Assoc ; 116(10): 748-754, 2017 Oct.
Article in English | MEDLINE | ID: mdl-28779848

ABSTRACT

BACKGROUND/PURPOSES: TGF-ß1 is an important growth factor that may influence the odontoblast differentiation and matrix deposition in the reactionary/reparative dentinogenesis to dental caries or other tooth injuries. TGF-ß1 exerts its effects through various signaling pathways, such as Smads and MAPKs. Cyclooxygenase-2 (COX-2) is a membrane-associated enzyme that produces prostaglandin E2 (PGE2) at sites of pulpal injury and inflammation, which leads to tissue swelling, redness and pain. The purposes of this study were to investigate the differential signal transduction pathways of TGF-ß1 that mediate COX-2 stimulation and PGE2 production in dental pulp cells. METHODS: Pulp cells were exposed to TGF-ß1 with/without SB431542 (an ALK5/Smad2 inhibitor) and U0126 (a MEK/ERK inhibitor). MTT assay was used to estimate cell viability. Enzyme-linked immunosorbent assay (ELISA) was used for measurement of PGE2 levels. RT-PCR and western blot were used to determined COX-2 mRNA and protein, respectively. RESULTS: Exposure to TGF-ß1 (1-10 ng/ml) increased the COX-2 mRNA and protein level of cultured pulp cells. Exposure to TGF-ß1 (0.1-10 ng/mL) significantly stimulated PGE2 production of dental pulp cells. Under the pretreatment of SB431542, the stimulatory effect of TGF-ß1 on COX-2 level of pulp cells was inhibited. Similarly, U0126 also partly inhibited the TGF-ß1-induced COX-2 expression. CONCLUSION: TGF-ß1 increased the COX-2 and PGE2 level of cultured pulp cells. The effect of TGF-ß1 on COX-2 protein expression was associated with ALK5/Smad2/3 and MEK/ERK pathways. These events are important in the early inflammation, repair and regeneration of dental pulp in response to injury.


Subject(s)
Cyclooxygenase 2/metabolism , Dinoprostone/metabolism , Epithelial Cells/cytology , Signal Transduction/drug effects , Transforming Growth Factor beta1/pharmacology , Benzamides/pharmacology , Butadienes/pharmacology , Cell Differentiation/drug effects , Cell Survival/drug effects , Cells, Cultured , Cyclooxygenase 2/genetics , Dental Pulp/cytology , Dental Pulp/drug effects , Dioxoles/pharmacology , Epithelial Cells/drug effects , Humans , Nitriles/pharmacology , Protein Serine-Threonine Kinases/metabolism , Receptor, Transforming Growth Factor-beta Type I , Receptors, Transforming Growth Factor beta/metabolism , Smad2 Protein/metabolism
4.
Oncotarget ; 7(13): 16879-94, 2016 Mar 29.
Article in English | MEDLINE | ID: mdl-26919242

ABSTRACT

Betel quid (BQ) chewing is an etiologic factor of oral submucous fibrosis (OSF) and oral cancer. There are 600 million BQ chewers worldwide. The mechanisms for the toxic and inflammatory responses of BQ are unclear. In this study, both areca nut (AN) extract (ANE) and arecoline stimulated epidermal growth factor (EGF) and interleukin-1α (IL-1α) production of gingival keratinocytes (GKs), whereas only ANE can stimulate a disintegrin and metalloproteinase 17 (ADAM17), prostaglandin E2 (PGE2) and 8-isoprostane production. ANE-induced EGF production was inhibited by catalase. Addition of anti-EGF neutralizing antibody attenuated ANE-induced cyclooxygenase-2 (COX-2), mature ADAM9 expression and PGE2 and 8-isoprostane production. ANE-induced IL-1α production was inhibited by catalase, anti-EGF antibody, PD153035 (EGF receptor antagonist) and U0126 (MEK inhibitor) but not by α-naphthoflavone (cytochrome p450-1A1 inhibitor). ANE-induced ADAM17 production was inhibited by pp2 (Src inhibitor), U0126, α-naphthoflavone and aspirin. AG490 (JAK inhibitor) prevented ANE-stimulated ADAM17, IL-1α, PGE2 production, COX-2 expression, ADAM9 maturation, and the ANE-induced decline in keratin 5 and 14, but showed little effect on cdc2 expression and EGF production. Moreover, ANE-induced 8-isoprostane production by GKs was inhibited by catalase, anti-EGF antibody, AG490, pp2, U0126, α-naphthoflavone, Zinc protoporphyrin (ZnPP) and aspirin. These results indicate that AN components may involve in BQ-induced oral cancer by induction of reactive oxygen species, EGF/EGFR, IL-1α, ADAMs, JAK, Src, MEK/ERK, CYP1A1, and COX signaling pathways, and the aberration of cell cycle and differentiation. Various blockers against ROS, EGF, IL-1α, ADAM, JAK, Src, MEK, CYP1A1, and COX can be used for prevention or treatment of BQ chewing-related diseases.


Subject(s)
Areca/toxicity , Gingiva/drug effects , Keratinocytes/drug effects , Plant Extracts/toxicity , Signal Transduction/drug effects , ADAM17 Protein/drug effects , ADAM17 Protein/metabolism , Cell Line , Dinoprost/analogs & derivatives , Dinoprost/metabolism , Dinoprostone/metabolism , Epidermal Growth Factor/drug effects , Epidermal Growth Factor/metabolism , Humans , Interleukin-1alpha/metabolism , Janus Kinases/drug effects , Janus Kinases/metabolism , Reactive Oxygen Species/metabolism
5.
Arch Oral Biol ; 61: 16-22, 2016 Jan.
Article in English | MEDLINE | ID: mdl-26492523

ABSTRACT

OBJECTIVE: Interleukin-1ß (IL-1ß) is an inflammatory molecule of the dental pulp. IL-1ß stimulates cyclooxygenase-2 (COX-2) and prostaglandins production of pulp cells and affects the pulpal inflammation and repair. However, the effects of IL-1ß on Monocyte Chemotactic Factor-1 (MCP-1) of dental pulp cells and its relation to transforming growth factor ß-activated kinase-1 (TAK1), PI3K/Akt, and MEK/ERK signaling and COX activation are not fully clear. DESIGN: Human dental pulp cells were exposed to IL-1ß with/without pretreatment and co-incubation by aspirin (a COX inhibitor), 5z-7-oxozeaenol (a TAK1 inhibitor), LY294002 (a PI3K/Akt inhibitor) or U0126 (a MEK/ERK inhibitor). Viable cell number was evaluated by MTT assay. MCP-1 mRNA expression was tested by reverse transcriptase-polymerase chain reaction (RT-PCR). MCP-1 and COX-2 protein expression was studied by western blot. MCP-1 in the culture medium was measure by ELISA. RESULTS: IL-1ß showed little cytotoxicity to pulp cells. It stimulated MCP-1 mRNA and protein expression and MCP-1 secretion. Aspirin, U0126, LY294002 and 5z-7-oxozeaenol attenuated the IL-1ß-induced MCP-1 expression. In addition, 5z-7-oxozeaenol, LY294002, U0126 and aspirin prevented the IL-1ß-induced MCP-1 secretion of pulp cells. CONCLUSION: These results indicate that IL-1ß may be involved in the pulpal inflammatory and healing processes by inducing MCP-1 expression and secretion. These events are related to differential activation of TAK1, PI3K/Akt and MEK/ERK 1/2 signaling and COX activation. These results are important for future pharmacologic intervention of pulpal inflammatory diseases.


Subject(s)
Chemokine CCL2/metabolism , Dental Pulp/cytology , Interleukin-1beta/pharmacology , MAP Kinase Signaling System/drug effects , Aspirin/pharmacology , Blotting, Western , Butadienes/pharmacology , Cells, Cultured , Chromones/pharmacology , Cyclooxygenase 2/metabolism , Enzyme-Linked Immunosorbent Assay , Humans , In Vitro Techniques , Morpholines/pharmacology , Nitriles/pharmacology , Reverse Transcriptase Polymerase Chain Reaction , Signal Transduction , Zearalenone/analogs & derivatives , Zearalenone/pharmacology
6.
PLoS One ; 9(8): e104310, 2014.
Article in English | MEDLINE | ID: mdl-25122505

ABSTRACT

Catechol (benzenediol) is present in plant-derived products, such as vegetables, fruits, coffee, tea, wine, areca nut and cigarette smoke. Because platelet dysfunction is a risk factor of cardiovascular diseases, including stroke, atherosclerosis and myocardial infarction, the purpose of this study was to evaluate the anti-platelet and anti-inflammatory effect of catechol and its mechanisms. The effects of catechol on cyclooxygenase (COX) activity, arachidonic acid (AA)-induced aggregation, thromboxane B2 (TXB2) production, lactate dehydrogenase (LDH) release, reactive oxygen species (ROS) production and extracellular signal-regulated kinase (ERK)/p38 phosphorylation were determined in rabbit platelets. In addition, its effect on IL-1ß-induced prostaglandin E2 (PGE2) production by fibroblasts was determined. The ex vivo effect of catechol on platelet aggregation was also measured. Catechol (5-25 µM) suppressed AA-induced platelet aggregation and inhibited TXB2 production at concentrations of 0.5-5 µM; however, it showed little cytotoxicity and did not alter U46619-induced platelet aggregation. Catechol (10-50 µM) suppressed COX-1 activity by 29-44% and COX-2 activity by 29-50%. It also inhibited IL-1ß-induced PGE2 production, but not COX-2 expression of fibroblasts. Moreover, catechol (1-10 µM) attenuated AA-induced ROS production in platelets and phorbol myristate acetate (PMA)-induced ROS production in human polymorphonuclear leukocytes. Exposure of platelets to catechol decreased AA-induced ERK and p38 phosphorylation. Finally, intravenous administration of catechol (2.5-5 µmole/mouse) attenuated ex vivo AA-induced platelet aggregation. These results suggest that catechol exhibited anti-platelet and anti-inflammatory effects, which were mediated by inhibition of COX, ROS and TXA2 production as well as ERK/p38 phosphorylation. The anti-platelet effect of catechol was confirmed by ex vivo analysis. Exposure to catechol may affect platelet function and thus cardiovascular health.


Subject(s)
Catechols/pharmacology , Cyclooxygenase Inhibitors/pharmacology , MAP Kinase Signaling System/drug effects , Platelet Aggregation Inhibitors/pharmacology , Reactive Oxygen Species/antagonists & inhibitors , Thromboxane A2/antagonists & inhibitors , p38 Mitogen-Activated Protein Kinases/antagonists & inhibitors , 15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid/pharmacology , Animals , Arachidonic Acid/pharmacology , Blood Platelets/drug effects , Blood Platelets/metabolism , Humans , Neutrophils/drug effects , Neutrophils/metabolism , Phosphorylation/drug effects , Platelet Aggregation/drug effects , Platelet Function Tests/methods , Prostaglandin-Endoperoxide Synthases/metabolism , Rabbits , Reactive Oxygen Species/metabolism , Thromboxane A2/metabolism , p38 Mitogen-Activated Protein Kinases/metabolism
7.
PLoS One ; 9(7): e101959, 2014.
Article in English | MEDLINE | ID: mdl-25051199

ABSTRACT

AIMS: Chewing of betel quid (BQ) increases the risk of oral cancer and oral submucous fibrosis (OSF), possibly by BQ-induced toxicity and induction of inflammatory response in oral mucosa. METHODS: Primary gingival keratinocytes (GK cells) were exposed to areca nut (AN) components with/without inhibitors. Cytotoxicity was measured by 3-(4,5-dimethyl- thiazol- 2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. mRNA and protein expression was evaluated by reverse transcriptase-polymerase chain reaction (RT-PCR) and western blotting. PGE2/PGF2α production was measured by enzyme-linked immunosorbent assays. RESULTS: Areca nut extract (ANE) stimulated PGE2/PGF2α production, and upregulated the expression of cyclooxygenase-2 (COX-2), cytochrome P450 1A1 (CYP1A1) and hemeoxygenase-1 (HO-1), but inhibited expression of keratin 5/14, cyclinB1 and cdc25C in GK cells. ANE also activated epidermal growth factor receptor (EGFR), Src and Ras signaling pathways. ANE-induced COX-2, keratin 5, keratin 14 and cdc25C expression as well as PGE2 production were differentially regulated by α-naphthoflavone (a CYP 1A1/1A2 inhibitor), PD153035 (EGFR inhibitor), pp2 (Src inhibitor), and manumycin A (a Ras inhibitor). ANE-induced PGE2 production was suppressed by piper betle leaf (PBL) extract and hydroxychavicol (two major BQ components), dicoumarol (a NAD(P)H: Quinone Oxidoreductase--NQO1 inhibitor) and curcumin. ANE-induced cytotoxicity was inhibited by catalase and enhanced by dicoumarol, suggesting that AN components may contribute to the pathogenesis of OSF and oral cancer via induction of aberrant differentiation, cytotoxicity, COX-2 expression, and PGE2/PGF2α production. CONCLUSIONS: CYP4501A1, reactive oxygen species (ROS), EGFR, Src and Ras signaling pathways could all play a role in ANE-induced pathogenesis of oral cancer. Addition of PBL into BQ and curcumin consumption could inhibit the ANE-induced inflammatory response.


Subject(s)
Areca/chemistry , Gene Expression/drug effects , Keratinocytes/metabolism , Plant Extracts/toxicity , Cells, Cultured , Curcumin/pharmacology , Cyclin B1/metabolism , Cyclooxygenase 2/genetics , Cyclooxygenase 2/metabolism , Cytochrome P-450 CYP1A1/metabolism , Dicumarol/pharmacology , Dinoprostone/biosynthesis , ErbB Receptors/metabolism , Gingiva/pathology , Heme Oxygenase-1/metabolism , Humans , Keratinocytes/drug effects , Keratins/genetics , Keratins/metabolism , Reactive Oxygen Species/metabolism , Signal Transduction , cdc25 Phosphatases/genetics , cdc25 Phosphatases/metabolism , ras Proteins/metabolism , src-Family Kinases/metabolism
8.
Carcinogenesis ; 34(5): 1096-104, 2013 May.
Article in English | MEDLINE | ID: mdl-23349021

ABSTRACT

Betel quid (BQ) chewing is an oral habit that increases the risk of oral cancer and oral submucous fibrosis (OSF), a precancerous condition showing epithelial atrophy and tissue fibrosis. Persistent fibroblast contraction may induce the fibrotic contracture of tissue. In this study, we found that areca nut extract (ANE) (200-1200 µg/ml) stimulated buccal mucosa fibroblast (OMF)-populated collagen gel contraction. Arecoline but not arecaidine-two areca alkaloids, slightly induced the OMF contraction. Exogenous addition of carboxylesterase (2U/ml) prevented the arecoline- but not ANE-induced OMF contraction. OMF expressed inositol triphosphate (IP3) receptors. ANE-induced OMF (800 µg/ml) contraction was inhibited by U73122 [phospholipase C (PLC) inhibitor] and 2-aminoethoxydiphenyl borate (IP3 receptor antagonist), respectively. Ethylene glycol tetraacetic acid and verapamil, two calcium mobilization modulators, also suppressed the ANE-induced OMF contraction. ANE induced calcium/calmodulin kinase II and myosin light chain (MLC) phosphorylation in OMF. Moreover, W7 (a Ca(2+)/calmodulin inhibitor), HA1077 (Rho kinase inhibitor), ML-7 (MLC kinase inhibitor) and cytochalasin B (actin filament polymerization inhibitor) inhibited the ANE-induced OMF contraction. Although ANE elevated reactive oxygen species (ROS) level in OMF, catalase, superoxide dismutase and N-acetyl-L-cysteine showed no obvious effect on ANE-elicited OMF contraction. These results indicate that BQ chewing may affect the wound healing and fibrotic processes in OSF via inducing OMF contraction by ANE and areca alkaloids. AN components-induced OMF contraction was related to PLC/IP3/Ca(2+)/calmodulin and Rho signaling pathway as well as actin filament polymerization, but not solely due to ROS production.


Subject(s)
Areca/adverse effects , Fibroblasts/pathology , Mouth Mucosa/pathology , Nuts/adverse effects , Oral Submucous Fibrosis/pathology , Precancerous Conditions/etiology , Precancerous Conditions/pathology , Arecoline/adverse effects , Arecoline/analogs & derivatives , Calcium/metabolism , Calcium-Calmodulin-Dependent Protein Kinase Type 2/genetics , Calcium-Calmodulin-Dependent Protein Kinase Type 2/metabolism , Cells, Cultured , Fibroblasts/metabolism , Humans , Inositol 1,4,5-Trisphosphate Receptors/genetics , Inositol 1,4,5-Trisphosphate Receptors/metabolism , Mouth Mucosa/metabolism , Mouth Neoplasms/etiology , Mouth Neoplasms/genetics , Mouth Neoplasms/metabolism , Mouth Neoplasms/pathology , Myosin Light Chains/genetics , Myosin Light Chains/metabolism , Oral Submucous Fibrosis/chemically induced , Oral Submucous Fibrosis/genetics , Oral Submucous Fibrosis/metabolism , Phosphorylation/genetics , Plant Extracts/adverse effects , Precancerous Conditions/genetics , Precancerous Conditions/metabolism , Reactive Oxygen Species/metabolism , Signal Transduction
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