ABSTRACT
The preparation and characterization of mixed-mode adsorbents for a typical separation purpose are of great importance in bioseparation areas. In this work, we prepared a new monolithic cryogel with a combination of ion-exchange and hydrophobic functions by employing benzyl-quaternary amine groups. The fundamental cryogel properties, protein equilibrium adsorption isotherm and chromatographic adsorption in the cryogel were measured experimentally. The results showed that, by using bovine serum album as the model protein, the dual functional cryogel has protein binding capability even in salt solution and the buffer with pH close or below the protein isoelectric point due to both the electrostatic and hydrophobic interactions. A capillary-based adsorption model was developed, which provided satisfied insights of the microstructure, axial dispersion, mass transfer as well as protein adsorption characteristics within the cryogel bed. The chromatographic isolation of bioactive proteins from rabbit blood serum was carried out by the cryogel. Immunoglobulin G antibody with a purity of 98.2% and albumin with a purity of 96.8% were obtained, indicating that the cryogel could be an interesting and promising adsorbent in bioseparation areas.
Subject(s)
Acrylic Resins/chemistry , Cryogels/chemistry , Immunoglobulin G/chemistry , Polystyrenes/chemistry , Quaternary Ammonium Compounds/chemistry , Serum Albumin/chemistry , Adsorption , Animals , Cattle , Chromatography, Liquid/methods , Cryogels/chemical synthesis , Hydrophobic and Hydrophilic Interactions , Ligands , Molecular Weight , RabbitsABSTRACT
OBJECTIVE: To analyze the early clinical effects of total hip arthroplasty(THA) for the treatment of old acetabular fractures. METHODS: From January 2007 to June 2010, thirteen patients with old acetabular fractures were reviewed, including 10 males and 3 females. Ten patients were treated with internal fixation and conservative treatment had been used in three patients. The average Harris Hip Score was used to evaluate therapeutic effects. RESULTS: After operation, all thirteen patients were followed up for one year. Hip X-ray films were taken and prosthesis loosening was not seen on any of the films at the 1st year after operation. The Harris Hip Score improved from preoperative (37.19 +/- 20.12) to postoperative (83.38 +/- 3.33), there was statistically significant difference. CONCLUSION: For reasons of malunion or failure of internal fixation, large and various bone defect, it's difficult to reach the anatomical reduction. THA is a good treatment method, but it needs rich skills and experience compared with ordinary operation.
Subject(s)
Acetabulum/injuries , Arthroplasty, Replacement, Hip/methods , Fractures, Bone/surgery , Female , Humans , Male , Middle Aged , Retrospective StudiesABSTRACT
OBJECTIVE: To explore the prognostic significance of hidden blood loss in total hip arthroplasty. METHODS: From May 2008 to July 2009, Harris hip score was used to evaluate the functions of 71 patients undergoing single side total hip arthroplasty (including 47 males and 24 females with a mean age of 68.3 years, ranged from 48 to 75 years). The blood loss in the operation was analyzed to study the correlation between hidden blood loss and the functional rehabilitation. RESULTS: All 71 patients undergoing THA were involved in the result analysis. The mean total blood loss was 1473 ml and the hidden blood loss was 545 ml (37%). Hidden blood loss significantly correlated with functional rehabilitation (P = 0.001), but there were no correlations between functional rehabilitation and age, gender, operative limb of patients (P = 0.067, 0.527, 0.926, 0.072). CONCLUSION: Hidden blood loss maybe a useful prognostic information contributing to the functional rehabilitation of total hip arthroplasty.
