ABSTRACT
A simple, optimized and sensitive high-performance liquid chromatograph method with ultraviolet (UV) detection (HPLC/UV) was developed and validated for determination of isochlorogenic acid A in rat plasma. The analytes were successfully separated on a Shodex C18 column (5 µm particle size, 250 mm × 4.6 mm, i.d.), the mobile phase contained 0.1% phosphoric acid aqueous solution (solvent A) and methanol (solvent B) (50:50, v/v) at a flow rate of 1.0 mL/min. The wavelength for UV detection was set at 300 nm and the column temperature was maintained in 30°C. Calibration curve for isochlorogenic acid A was found to be good linear over the range of 0.04-40 µg/mL (r = 0.9998). The intra- and inter-day precisions (relative standard deviation) were within 7.63% and the assay accuracy (RE) ranged from -1.41 to 3.25%. The limit of detection and the lower limit of quantification were 0.012 and 0.04 µg/mL, respectively. The validated method was successfully applied to pharmacokinetic study of isochlorogenic acid A in rats for the first time. The pharmacokinetic parameters were evaluated after the rats were administered intravenously and intragastrically isochlorogenic acid A at the single dose of 18 mg/kg, respectively. The absolute bioavailability was calculated to be 22.6%.
Subject(s)
Chlorogenic Acid/analogs & derivatives , Chromatography, High Pressure Liquid/methods , Animals , Chlorogenic Acid/blood , Chlorogenic Acid/chemistry , Chlorogenic Acid/pharmacokinetics , Limit of Detection , Linear Models , Male , Rats , Rats, Sprague-Dawley , Reproducibility of ResultsABSTRACT
Context Oxymatrine (OMT) is beneficial to human health by exerting various biological effects. Objective To investigate the absorption mechanism of OMT and discover absorption enhancers using Madin-Darby canine kidney (MDCK) cell monolayers. Materials and methods Concentration effects on the transport of OMT were measured in the range of 1.0 × 10(-5)-1.0 × 10(-3) M in 2 h. Then, the effect of time, direction, temperature and pH on the transport of OMT at 10(-4) M was studied. Moreover, Papp of OMT was determined in the absence/presence of cyclosporine and surfactants at 100 µM to further confirm the relative transport mechanism. Results The Papp APâBL ranged from (3.040 ± 0.23) × 10(-6) to (3.697 ± 0.19) × 10(-6 )cm/s as the concentration varied from 10(-5) to 10(-3) M. OMT showed similar Papp at 4 and 37 °C (p > 0.05). Increasing the apical pH 7.4 and 8.0 resulted in Papp versus pH 5.0 (p < 0.01). Furthermore, in the presence of cyclosporine and surfactants including sodium citrate, sodium dodecyl sulphate (SDS) and deoxysodium cholate, Papp was (0.318 ± 0.033) × 10(-5), (0.464 ± 0.048) × 10(-5), (0.897 ± 0.115) × 10(-5) and (1.341 ± 0.122) × 10(-5 )cm/s, respectively. In the presence of surfactants, Papp significantly increased up to 1.5-4.3-fold (p < 0.05). Discussion and conclusion OMT transport across MDCK cell monolayers was by passive diffusion. Sodium citrate, SDS and deoxysodium cholate serve as excellent absorption enhancers which are useful for the related research improving the oral bioavailability of OMT.
Subject(s)
Alkaloids/metabolism , Epithelial Cells/metabolism , Kidney/metabolism , Quinolizines/metabolism , Renal Reabsorption , Animals , Citrates/pharmacology , Cyclosporine/pharmacology , Deoxycholic Acid/pharmacology , Diffusion , Dogs , Dose-Response Relationship, Drug , Epithelial Cells/drug effects , Hydrogen-Ion Concentration , Kidney/drug effects , Kinetics , Linear Models , Madin Darby Canine Kidney Cells , Permeability , Renal Reabsorption/drug effects , Sodium Citrate , Sodium Dodecyl Sulfate/pharmacology , Surface-Active Agents/pharmacology , TemperatureABSTRACT
A simple and reliable high performance liquid chromatography coupled with electrospray ionization mass spectrometry (HPLC-ESI-MS) analysis method was established to simultaneously determine thirteen flavonoids of Xiaobuxing-Tang in intestine perfusate, namely onpordin, 3'-O-methylorobol, glycitein, patuletin, genistein, luteolin, quercetin, nepitrin, quercimeritrin, daidzin, patulitrin, quercetagitrin and 3-glucosylisorhamnetin. Detection was performed on a quadrupole mass spectrometer equipped with an electrospray ionization (ESI) source operating in negative ionization mode. Negative ion ESI was used to form deprotonated molecules at m/z 315 for onpordin, m/z 299 for 3'-O-methylorobol, m/z 283 for glycitein, m/z 331 for patuletin, m/z 269 for genistein, m/z 285 for luteolin, m/z 301 for quercetin, m/z 477 for nepitrin, m/z 463 for quercimeritrin, m/z 461 for daidzin, m/z 493 for patulitrin, m/z 479 for quercetagitrin, m/z 477 for 3-glucosylisorhamnetin and m/z 609.2 for rutin. The linearity, sensitivity, selectivity, repeatability, accuracy, precision, recovery and matrix effect of the assay were evaluated. The proposed method was successfully applied to simultaneous determination of these thirteen flavonoids, and using this method, the intestinal absorption profiles of thirteen flavonoids were preliminarily predicted.
