Plant size-dependent influence of foliar fungal pathogens promotes diversity through allometric growth.

The effect of pathogens on host diversity has attracted much attention in recent years, yet how the influence of pathogens on individual plants scales up to affect community-level host diversity remains unclear. Here, we assessed the effects of foliar fungal pathogens on plant growth and species richness using allometric growth theory in population-level and community-level foliar fungal pathogen exclusion experiments. We calculated growth scaling exponents of 24 species to reveal the intraspecific size-dependent effects of foliar fungal pathogens on plant growth. We also calculated the intercepts to infer the growth rates of relatively larger conspecific individuals. We found that foliar fungal pathogens inhibited the growth of small conspecific individuals more than large individuals, resulting in a positive allometric growth. After foliar fungal pathogen exclusion, species-specific growth scaling exponents and intercepts decreased, but became positively related to species' relative abundance, providing a growth advantage for individuals of abundant species with a higher growth scaling exponent and intercept compared with rare species, and thus reduced species diversity. By adopting allometric growth theory, we elucidate the size-dependent mechanisms through which pathogens regulate species diversity and provide a powerful framework to incorporate antagonistic size-dependent processes in understanding species coexistence.

Exposure risk assessment of representative phthalate acid esters and associated plastic debris under the agricultural land use in typical Chinese regions.

Phthalate acid esters (PAEs) are frequently detected in the global environment and can cause potential health hazards. In this study, quantitative exposure risk assessment was undertaken to derive soil generic assessment criteria (GAC) for six representative PAEs under the agricultural land use in the evaluated Chinese regions, which coupled multi-media transport and human exposure models based on multiple exposure pathways including vegetables consumption, dermal absorption, ingestion of soil and dust, and the exposure from non-soil sources. It is identified that the PAEs in agricultural soil are dominated by DEHP and DnBP representing 72-96% of the total PAEs. The GAC for BBP and DEHP, calculated on the basis of region-specific exposure parameters and soil properties in various locations, are stringent, signifying greater potential health risks from exposure to them, warranting more rigorous contamination management. The proposed soil GAC for plastic debris are 100, 107, 73 and 88 mg kg-1 for Heilongjiang Province, Beijing City, Jiangsu and Guangdong Provinces respectively. Additionally, the potential risks of 1.68 × 10-6 and 7 × 10-6 are identified for BBP and DEHP in Guangdong Province as indicated by the exceedance of target risk level of 1 × 10-6, with the consumption of vegetables being the dominant contributor to the total estimated PAEs exposure. Overall, this methodology based on the coupled contaminant transport and exposure models incorporating region-specific data provides a technical framework to derive science-based soil GAC for representative PAEs for maintaining and assessing soil quality and food safety under the agricultural land use.

Human Health Risk Assessment of Elevated Fe and Mn Intake in Groundwater in Yangtze Catchment.

Globally, it has been reported that groundwater contains elevated levels of Fe and Mn. However, the risk of prolonged exposure to groundwater with elevated Fe and Mn was often ignored due to their much lower carcinogenic risk. To assess the human health risk of elevated Fe and Mn intake in groundwater, 1863 groundwater samples from the Yangtze catchment, a densely populated and economically prosperous area of China, were collected in this study. The spatial distributions of Fe and Mn in groundwater were investigated by the geographic information system (GIS) and their health risk assessment was done. The results indicated that 38.6% and 50.3% of the groundwater samples were defined as "elevated/high" levels for Fe and Mn, respectively, exceeding 0.3 and 0.1 mg/L (World Health Organization guidelines). Moreover, in the groundwater of Yangtze Catchment, the order of Fe and Mn contents is followed by upper< middle< lower. Based on the calculated hazard index (HI), HIadult and HIchild were in a range of 0-4.91 and 0-11.07, respectively. There was an area of 3,483 and 35,523 km2 with a non-carcinogenic risk from Fe and Mn, correspondingly. The numbers of affected adults and children were about 3,018,066 and 2,775,007, respectively. It means that 0.20% and 2.00% of the study area or 0.64% and 0.59% of the total population will suffer health risks from Fe and Mn intake in groundwater, respectively. Therefore, a significant basis for groundwater safety in the Yangtze catchment and similar areas was provided in this study.

Host plant height explains the effect of nitrogen enrichment on arbuscular mycorrhizal fungal communities.

Nitrogen (N) enrichment is widely known to affect the root-associated arbuscular mycorrhizal fungal (AMF) community in different ways, for example, via altering soil properties and/or shifting host plant functional structure. However, empirical knowledge of their relative importance is still lacking. Using a long-term N addition experiment, we measured the AMF community taxonomic and phylogenetic diversity at the single plant species (roots of 15 plant species) and plant community (mixed roots) levels. We also measured four functional traits of 35 common plant species along the N addition gradient. We found divergent responses of AMF diversity to N addition for host plants with different innate heights (i.e. plant natural height under unfertilized treatment). Furthermore, our data showed that species-specific responses of AMF diversity to N addition were negatively related to the change in maximum plant height. When scaling up to the community level, N addition affected AMF diversity mainly through increasing the maximum plant height, rather than altering soil properties. Our results highlight the importance of plant height in driving AMF community dynamics under N enrichment at both species and community levels, thus providing important implications for understanding the response of AMF diversity to anthropogenic N deposition.

Coexistence is stabilized by conspecific negative density dependence via fungal pathogens more than oomycete pathogens.

Plant pathogens are often hypothesized to promote species coexistence by generating conspecific negative density dependence (CNDD). However, the relative importance of fungal versus oomycete pathogens in maintaining plant species coexistence and community composition remains unresolved, despite their recognized effects on plant performance. Here, we use fungicide application to investigate how fungal versus oomycete pathogens affect plant species coexistence in an alpine meadow. We found that the severity of foliar fungal disease was density-dependent at both intra- and interspecific levels. Fungal pathogen-exclusion treatment successfully decreased the severity of foliar fungal diseases, with no detectable effects on root colonization by arbuscular mycorrhizal fungi or on soil chemical properties. Fungal pathogens were important factors shaping CNDD across 25 coexisting plant species. Exclusion of fungal pathogens significantly reduced plant species richness and Shannon's evenness. Treatments that excluded fungal pathogens also led to significant shifts in plant community composition toward more Poaceae and Cyperaceae. These results indicate that fungal pathogens, especially those affecting aboveground plant parts, may play a larger role in maintaining species coexistence and shaping community composition than has been previously recognized.