Interactive effects of grassland utilization and climatic factors govern the plant diversity-soil C relationship in steppe of North China.

The relationship between plant diversity and the ecosystem carbon pool is important for understanding the role of biodiversity in regulating ecosystem functions. However, it is not clear how the relationship between plant diversity and soil carbon content changes under different grassland use patterns. In a 3-year study from 2013 to 2015, we investigated plant diversity and soil total carbon (TC) content of grasslands in northern China under different grassland utilization methods (grazing, mowing, and enclosure) and climatic conditions. Shannon-Wiener and Species richness index of grassland were significantly decreased by grazing and mowing. Plant diversity was positively correlated with annual precipitation (AP) and negatively correlated with annual mean temperature (AMT). AP was the primary regulator of plant diversity. Grazing and mowing decreased TC levels in grasslands compared with enclosures, especially in topsoil (0-20 cm). The average TC content was decreased by 58 % and 36 % in the 0-10 cm soil layer, while it was decreased by 68 % and 39 % in 10-20 cm soil layer. TC was positively correlated with AP and negatively correlated with AMT. Principal component analysis (PCA) showed that plant diversity was positively correlated with soil TC, and the correlation decreased with an increase in the soil depth. Overall, this study provides a theoretical basis for predicting soil carbon storage in grasslands under human disturbances and climate change impacts.

Empowering environmental change: role of green bonds in shaping green e-commerce and sustainable business.

This research aims to investigate the impact of green bonds on the development of sustainable and environmentally friendly e-commerce businesses, with a focus on promoting environmental empowerment. Recognizing the necessity of preparing for economic changes and strengthening institutional frameworks, this study addresses the shortcomings identified in previous research, including statistical limitations, insufficient inclusion of variables, and a lack of diversity. By bridging these gaps, the research explores the interconnections between economic expansion, sustainability, profitability, and effective governance. To account for country-specific variations, a robust reduced-form modeling strategy is employed, supplemented by generalized method of moments estimates to address potential variability. The study analyzes survey data spanning the years 1990 to 2019. The research findings confirm the significant role of organizational excellence and economic growth in fostering sustainability, lending support to the presence of the environmental Kuznets curve hypothesis. Additionally, the study underscores the potential adverse consequences for the natural environment if economic liberalization proceeds without robust governance structures. By providing original empirical evidence on the effectiveness of green bonds in driving the growth of sustainable e-commerce businesses, this research contributes to the field of environmental empowerment and emphasizes the importance of well-designed frameworks to guide economic transitions. The insights derived from this study have implications for policymakers, businesses, and organizations aiming to align economic growth with sustainability goals, ultimately facilitating a more environmentally conscious and resilient future.

Assessment of the vegetation sensitivity index in alpine meadows with a high coverage and toxic weed invasion under grazing disturbance.

Maintaining healthy ecosystems is essential to ensure sustainable socio-economic development. Studies combining remote sensing data with grassland health assessments, extensively performed at different scales, are important for monitoring grassland health from a spatiotemporal perspective to enable scientific grazing management. However, most studies only use quantitative grassland degradation indices, such as grassland cover; this is done despite the fact that some degraded grasslands maintain a high level of cover solely by virtue of the proliferation of toxic weeds. Thus, seeking indices that are a more accurate representation of the health status of grassland vegetation is of utmost importance. Therefore, in order to accurately characterize the ecological integrity of grasslands (i.e., while limiting the impact of confounding variables such as weeds), we chose the grassland health comprehensive evaluation index VOR (vigor, organization, and resilience) to assess the health of grasslands on the Tibetan Plateau. We applied the VOR evaluation indices to two rangelands with different grazing intensity on the Tibetan Plateau, and extracted 11 commonly used vegetation indices based on remote sensing images of rangelands,then modeled them with the data from field surveys. Our results show that the FVC, PS, and VOR were higher in lightly grazed pastures than in heavily grazed pastures in the 2017 and 2018 growing seasons. At the beginning of the sampling period, Poaceae accounted for a greater proportion in the HG pasture. However, by August 2018, the proportion of Poaceae in the LG pasture exceeded that in the HG pasture. the proportion of Forbs in the HG pasture was significantly greater than that in the LG pasture. This indicates that vegetation response to grazing disturbance is not only a volume reduction but also a vegetation composition change. The ratio vegetation index was the most sensitive to the vegetation health response, enabling the quantification and prediction of regional vegetation health and objectively reflecting the actual condition of the grassland ecosystem. According to a multiple regression analysis, the main climatic limiting factor in the region is precipitation, which positively correlated with VOR; whereas, grazing disturbance is an important driving factor, and it is inversely correlated with VOR.

Growth-Defense Trade-Offs Induced by Long-term Overgrazing Could Act as a Stress Memory.

Long-term overgrazing (OG) is one of the key drivers of global grassland degradation with severe loss of productivity and ecosystem functions, which may result in stress memory such as smaller stature of grassland plants. However, how the OG-induced stress memory could be regulated by phytohormones is unknown. In this study, we investigated the changes of four phytohormones of cloned offspring of Leymus chinensis that were developed from no-grazing (NG) plants and OG plants with a grazing history of 30 years. The concentrations of auxin (IAA) and gibberellic acid (GA) in OG plant leaves were 45% and 20% lower than control, respectively. Meanwhile, the level of abscisic acid (ABA) in OG leaves nearly doubled compared with that in NG leaves. The situation was quite similar in roots. Unexpectedly, no significant changes in the jasmonic acid (JA) level were observed between OG and NG plants. The changes in gene expression patterns between OG and NG plants were also investigated by transcriptomic analysis. In total, 302 differentially expressed genes (DEGs) were identified between OG and NG plants, which were mainly classified into the functions of synthesis, receptor, and signal transduction processes of phytohormones. The expression of 24 key genes related to the biosynthesis and signal transduction of IAA and GA was downregulated in OG plants. Among them, OASA1 and AO1 (regulating the biosynthesis of IAA and ABA, respectively) were reduced significantly by 88 and 92%, respectively. In addition, the content of secondary metabolites related to plant defense such as flavonoids and phenols was also increased in leaves. Taken together, the decrease of positive plant growth-related hormones (IAA and GA) together with the increase of plant stress-related hormones or factors (ABA, flavonoids, and phenols) induced the growth-defense trade-offs for L. chinensis adaptation to long-term OG stress. The findings reported in this study shed new light on the mechanism of plant-animal interaction in the grassland ecosystem and provide a deeper insight into optimizing grazing management and sustainable utilization of grassland.

Multi-Cutting Improves Forage Yield and Nutritional Value and Maintains the Soil Nutrient Balance in a Rainfed Agroecosystem.

Increasing forage yield and nutritional quality under the premise of maintaining relatively stable land area and soil nutrient content is a necessary condition for the sustainable development of grassland animal husbandry. Different cutting models [simulated grazing (SG), hay harvesting (H)] of oat (Avena sativa), common vetch (Vicia sativa) and their mixture (Avena sativa + Vicia sativa) were studied on the Loess Plateau. The results show that (1) SG could increase forage yield, crude protein, and crude fat content and decrease crude ash content. In 2014, the yield of Avena sativa per hectare was 3,578.11 kg higher than that of H; (2) the model analysis for predicting nutritional components showed that the Crude protein (CP) and EE contents of forages in each variety (combination) showed a linear downward trend with increasing forage yield. Redundancy analysis showed that precipitation, especially in the growing season, was positively correlated with grass yield and CP content; and (3) there were significant differences in soil organic carbon, total nitrogen, NO3 --N, and NH4 +-N contents for the different forage varieties (combinations) under different use modes; the values first decreased, then increased, and finally decreased. According to the comprehensive evaluation value calculated by Technique for Order Preference by Similarity to an Ideal Solution, mixed sowing was better than monoculture, and SG obtained better results than H. Overall, mixed sowing under SG can improve forage yield and nutritional quality. At the same time, precipitation regulation is the key factor affecting the production performance of rainfed cultivated grassland on the Loess Plateau.

Comparison of the Responses of Soil Fungal Community to Straw, Inorganic Fertilizer, and Compost in a Farmland in the Loess Plateau.

The Loess Plateau is located in the arid and semi-arid regions in northern China. The ecosystem is particularly sensitive to natural and anthropogenic disturbances. Fungi can produce extracellular enzymes, decompose a variety of organic matter, and regulate carbon and nutrient balance. We studied the changes of soil fungal community compositions in response to straw, inorganic fertilizer, and compost in a typical farmland in the Loess Plateau. Our results demonstrated that the addition of straw significantly reduces the Shannon index of the fungal community, in addition, the participation of straw significantly affects the composition of the fungal community. Functional prediction based on FUNGuild showed that straw significantly reduced the relative abundance of saprotrophs, pathotrophs, symbiotrophs, lichenized, ectomycorrhizal, and plant pathogens. Although fertilization practices destroyed the co-occurrence pattern among the fungal species, the addition of straw alleviated this affect. No significant effect of straw, compost, and inorganic fertilizers on the co-occurrence pattern among species in the soil fungal community was observed. Compared with compost and inorganic fertilizer, the addition of straw shaped the community composition by changing the relative abundance of fungal functional taxa. Thus, in the fragile Loess Plateau environment, over-fertilizing or non-order-fertilizing may destroy the co-occurrence pattern of the fungal communities and Loess Plateau ecosystem. IMPORTANCE Determining the response of soil fungi in sensitive ecosystems to external environmental disturbances is an important, yet little-known, topic in microbial ecology. In this study, we evaluated the impact of traditional fertilization management practices on the composition, co-occurrence pattern, and functional groups of fungal communities in loessial soil. Our results show that in the fragile Loess Plateau environment, fertilizer management changed the composition of the fungal community and disrupted the co-occurrence pattern between fungi. The application of straw alleviates the destroying of the co-occurrence pattern. The current research emphasizes the necessity of rational fertilization of farmland in loessial soil.

Synthesis of MOF-derived Ni@C materials for the electrochemical detection of histamine.

Histamine (HA) plays an important role in food safety supervision and is also involved in various physiological functions. Accurate and rapid detection of HA in real sample is count for much as this is the significant prerequisite for its effective monitoring. In this study, we fabricated an electrochemical sensor to detect HA via the pyrolysis of the hydrothermal Ni-MOF (metal-organic frameworks), in which the obtained Ni@C material was deployed as the sensing agent. Ni@C was comprehensively characterized in terms of its morphology, constitution, as well as its electrochemical behavior. The as-prepared sensor (Ni@C/GCE) features excellent electrocatalytic activities. It was also observed that the electrochemical property of the sensor was substantially improved because Ni@C afforded an enlarged active surface and accelerated electron transport. This sensor affords amperometric analysis in the linear range of 10-3-100 µM HA with a 3.2 × 10-4 µM low detection limit (S/N = 3). Many important features, including decent anti-interference, reproducibility, stability, and reliability, were also observed. Importantly, the sensor enabled the measurement of HA in real samples obtained from fish, thus demonstrating its practical potential as a HA analytical detector.

Productivity and Quality of Alpine Grassland Vary With Soil Water Availability Under Experimental Warming.

The plant productivity of alpine meadow is predicted to generally increase under a warming climate, but it remains unclear whether the positive response rates will vary with soil water availability. Without consideration of the response of community composition and plant quality, livestock grazing under the current stocking rate might still lead to grassland degradation, even in meadows with high plant biomass. We have conducted a warming experiment from 2010 to 2017 to examine the interactive effects of warming and soil water availability on plant growth and forage quality at individual and functional group levels in an alpine meadow located in the permafrost region of the Qinghai-Tibetan Plateau. Warming-induced changes in community composition, biomass, and forage quality varied with soil water availability. Under dry conditions, experimental warming reduced the relative importance of grasses and the aboveground biomass by 32.37 g m-2 but increased the importance value of forbs. It also increased the crude fat by 0.68% and the crude protein by 3.19% at the end of summer but decreased the acid detergent fiber by 5.59% at the end of spring. The increase in crude fat and protein and the decrease in acid detergent fiber, but the decrease in aboveground biomass and increase the importance value of forbs, which may imply a deterioration of the grassland. Under wet conditions, warming increased aboveground biomass by 29.49 g m-2 at the end of spring and reduced acid detergent fiber by 8.09% at the end of summer. The importance value of grasses and forbs positively correlated with the acid detergent fiber and crude protein, respectively. Our results suggest that precipitation changes will determine whether climate warming will benefit rangelands on the Qinghai-Tibetan Plateau, with drier conditions suppressing grassland productivity, but wetter conditions increasing production while preserving forage quality.

Bacterial and fungal communities and contribution of physicochemical factors during cattle farm waste composting.

During composting, the composition of microbial communities is subject to constant change owing to interactions with fluctuating physicochemical parameters. This study explored the changes in bacterial and fungal communities during cattle farm waste composting and aimed to identify and prioritize the contributing physicochemical factors. Microbial community compositions were determined by high-throughput sequencing. While the predominant phyla in the bacterial and fungal communities were largely consistent during the composting, differences in relative abundances were observed. Bacterial and fungal community diversity and relative abundance varied significantly, and inversely, over time. Relationships between physicochemical factors and microbial community compositions were evaluated by redundancy analysis. The variation in bacterial community composition was significantly related to water-soluble organic carbon (WSOC), and pile temperature and moisture (p < .05), while the largest portions of variation in fungal community composition were explained by pile temperature, WSOC, and C/N (p < .05). These findings indicated that those parameters are the most likely ones to influence, or be influenced by the bacterial and fungal communities. Variation partitioning analyses indicated that WSOC and pile temperature had predominant effects on bacterial and fungal community composition, respectively. Our findings will be useful for improving the quality of cattle farm waste composts.

Bacterial and Fungal Community Structures in Loess Plateau Grasslands with Different Grazing Intensities.

The Loess Plateau of China is one of the most fragile ecosystems worldwide; thus, human production activities need to be conducted very cautiously. In this study, MiSeq high-throughput sequencing was applied to assess the relationship between bacterial and fungal community structures and changes in vegetation and soil physical and chemical properties induced by grazing, in four grasslands with different levels of grazing intensity (0, 2.67, 5.33, and 8.67 sheep/ha) in the semiarid region of the Loess Plateau. The relative abundances of the bacterial community in the grasslands with 2.67 and 5.33 sheep/ha were significantly higher than those in grasslands with 0 and 8.67 sheep/ha, and the fungal diversity was significantly lower for grasslands with 2.67 sheep/ha than for the other grasslands. Redundancy analysis (RDA) showed that plant biomass, nitrate, and total nitrogen have significant effects on bacterial community structure, whereas nitrate and total nitrogen also significantly affect fungal community structure. Variation partitioning showed that soil and plant characteristics influence the bacterial and fungal community structures; these characteristics explained 51.9 and 52.9% of the variation, respectively. Thus, bacterial and fungal community structures are very sensitive to grazing activity and change to different extents with different grazing intensities. Based on our findings, a grazing intensity of about 2.67 sheep/ha is considered the most appropriate in semiarid grassland of the Loess Plateau.

Microbial Nitrogen-Cycle Gene Abundance in Soil of Cropland Abandoned for Different Periods.

In Inner Mongolia, steppe grasslands face desertification or degradation because of human overuse and abandonment after inappropriate agricultural management. The soils in these abandoned croplands exist in heterogeneous environments characterized by widely fluctuating microbial growth. Quantitative polymerase chain reaction analysis of microbial genes encoding proteins involved in the nitrogen cycle was used to study Azotobacter species, nitrifiers, and denitrifiers in the soils from steppe grasslands and croplands abandoned for 2, 6, and 26 years. Except for nitrifying archaea and nitrous oxide-reducing bacteria, the relative genotypic abundance of microbial communities involved in nitrogen metabolism differed by approximately 2- to 10-fold between abandoned cropland and steppe grassland soils. Although nitrogen-cycle gene abundances varied with abandonment time, the abundance patterns of nitrogen-cycle genes separated distinctly into abandoned cropland versus light-grazing steppe grassland, despite the lack of any cultivation for over a quarter-century. Plant biomass and plant diversity exerted a significant effect on the abundance of microbial communities that mediate the nitrogen cycle (P < 0.002 and P < 0.03, respectively). The present study elucidates the ecology of bacteria that mediate the nitrogen cycle in recently abandoned croplands.

Effects of stocking rates on functional group diversity and forage quality in rangeland of Qilian Mountain, China.

The present study aimed at investigating a balance between environment and livestock grazing, through identifying appropriate stocking rate in rangeland with highest biodiversity and forage quality. The experiment was carried out to determine the effects of six stocking rates on Shannon Weiner index of functional group diversity and nutritive value and relationship between them in the edge of the Tibetan plateau of China. The results showed that abundance of functional group diversity indices were significantly influenced by stocking rates (p < 0.05) and the highest appeared in 2.5 and 2.6 animal month unit (AMU) ha(-1)'. There were significant differences in forage content of nitrogen (N), water-soluble carbohydrate (WSC), neutral detergent fiber (NDF) and acid detergent fiber (ADF) under stocking rates (p<0.05). There were higher N and WSC content but lower NDF and ADF content under 2.5 and 2.6 AMU ha(-1) than other stocking rates. Positive relationship was found between all functional group diversity indices and N and WSC content of community but negative relationship between all functional group diversity indices and NDF and ADF content of community. All the results represented that moderate control of stocking rates was an effective management measure to protect functional diversity, improve forage quality and sustain rangeland health.

Effect of abandonment on diversity and abundance of free-living nitrogen-fixing bacteria and total bacteria in the cropland soils of Hulun Buir, Inner Mongolia.

In Inner Mongolia, steppe grasslands face desertification or degradation because of human over activity. One of the reasons for this condition is that croplands have been abandoned after inappropriate agricultural management. The soils in these croplands present heterogeneous environments in which conditions affecting microbial growth and diversity fluctuate widely in space and time. In this study, we assessed the molecular ecology of total and free-living nitrogen-fixing bacterial communities in soils from steppe grasslands and croplands that were abandoned for different periods (1, 5, and 25 years) and compared the degree of recovery. The abandoned croplands included in the study were natural restoration areas without human activity. Denaturing gradient gel electrophoresis and quantitative PCR (qPCR) were used to analyze the nifH and 16S rRNA genes to study free-living diazotrophs and the total bacterial community, respectively. The diversities of free-living nitrogen fixers and total bacteria were significantly different between each site (P<0.001). Neither the total bacteria nor nifH gene community structure of a cropland abandoned for 25 years was significantly different from those of steppe grasslands. In contrast, results of qPCR analysis of free-living nitrogen fixers and total bacteria showed significantly high abundance levels in steppe grassland (P<0.01 and P<0.03, respectively). In this study, the microbial communities and their gene abundances were assessed in croplands that had been abandoned for different periods. An understanding of how environmental factors and changes in microbial communities affect abandoned croplands could aid in appropriate soil management to optimize the structures of soil microorganisms.

Effects of Buyang Huanwu Decoction on antioxidant and drug-metabolizing enzymes in rat liver.

AIM: To study the effect of Buyang Huanwu Decoction (BYHWD) on the antioxidant enzymes and drug-metabolizing enzymes in rat liver. METHOD: Following treatment of rats with BYHWD at 6.42, 12.83, or 25.66 g·kg(-1) per day for 15 days, microsomes and cytosols isolated from the liver tissues were prepared by differential centrifugation according to standard procedures. The activities of the antioxidant enzymes and cytochrome b5, NADPH-cytochrome P450 reductase, CYP3A, CYP2E1, UGT, and GST of the rat livers were determined by UV-Vis spectrophotometer. RESULTS: The activities of ALT, AST, antioxidant enzymes, and the Hepatosomatic Index in serum were not significantly affected. In cytosols, the activity of CAT was significantly increased at the dosage of 12.83 g·kg(-1), and all the other antioxidant activities and MDA levels were not affected by this treatment. BYHWD had no effect on cytochrome b5, NADPH-cytochrome P450 reductase, CYP3A, and UGT. At the highest dose (25.66 g·kg(-1)), the activity of CYP2E1 was significantly inhibited, and the activities of GST and the level of GSH were increased. CONCLUSION: BYHWD is safe for the liver, and has the functions of detoxification and antioxidant. Patients should be cautioned about the herb-drug interaction of BYHWD and CYP2E1 substrates.

[Effects of xuefu zhuyu decoction on antioxidant and drug-metabolizing enzymes in liver of rats].

Xuefu Zhuyu decoction (XFZYD) is a famous traditional Chinese medicine (TCM) formula, is widely used in the treatment of cardiovascular and cerebrovascular diseases in China over one hundred years. But its effect on antioxidant and drug-metabolizing enzymes are unknown. This study was to observe the effects of Xuefu Zhuyu decoction (XFZYD) on the activities of antioxidant and drug metabolism enzymes (DMEs) in liver of rats. Male SD rats, treated with XFZYD at the dosage of 3.51, 7.02 and 14.04 g x kg(-1) per day for 15 days, serum were collected, tissue fluid, cytosols and microsomes isolated from liver tissues were prepared by centrifugation according to the standard procedure, the activities of antioxidant enzymes and drug-Metabolizing Enzymes were determined by UV-V is spectrophotometer. In serum, the activities of AST was not significantly affected by the treatment with XFZYD, at the high- est dose, the levels of ALT, Cr and BUN were significantly decreased (P < 0.05). GPX were significantly increased at the dose of 7.02, 14.04 g x kg(-1) (P < 0.05), CAT were significantly increased at the highest dose (P < 0.05). T-SOD was not significantly af- fected by this treatment. In the liver tissue, GPX was significantly increased at the dose of 3.51, 7.02 g x kg(-1) (P < 0.05), GST, CAT and T-SOD were not significantly affected following this treatment. In cytosols, GST was significantly increased at the dose of 3.51 g x kg(-1) (P < 0.05), T-SOD was remarkable induced at the dose of 3.51 and 7.02 g x kg(-1) (P < 0.05). In microsomes, XFZYD had no significant effect on Cytochromeb5, NADPH-Cytochrome P450 reductase, CYP3A, CYP2E1 and UGT, XFZYD significantly in- duced GST at the dose of 3.51 and 7.02 g x kg(-1) (P < 0.05), and the level of GSH were significantly increased by XFZYD at the dose of 3.51, 7.02 and 14.04 g kg(-1) (P < 0.05). These findings suggest XFZYD can induce the activities of GPX, CAT, SOD, GST and increase GSH level in liver of rats, which indicate XFZYD may have detoxification and antioxidant functions.

[The construction of lentivirus-mediated RNAi vector containing hTERT].

OBJECTIVE: To construct a recombinant lentivirus RNA interference (RNAi) vector carrying hTERT gene, and to obtain the titer of the lentiviral stock for investigating the expression in the eukaryotic cells and the effect on the hTERT gene silencing in the eukaryotic cells. METHODS: Two complimentary oligos of small interference RNA (siRNA) with hairpin structures targeting the hTERT gene and a negative control were synthesized, then ligated with pLVTHM vector and sequenced. The recombinant vectors were then transfected with viral packaging mix into T293 cells, viral supernatant was harvested to determine the titer. U87 cells infected by virus were harvested and the expression of hTERT, telomerase activity and apoptosis were detected by reverse transcription-PCR(RT-PCR), TRAP assay and flow cytometry separately. RESULTS: Sequencing data showed that the constructed plasmids contained the correct sequences of hTERT siRNA transcript templates. A vector producing cell line T293 was established, and the titer for transfection was obtained. RT-PCR and TRAP flow cytometry analyses demonstrated that hTERT shRNA expression construct could suppress the expression of hTERT and telomerase activity and induce apoptosis. CONCLUSION: A lentivirus RNAi vector targeting hTERT gene was successfully constructed, which decreased the expression of hTERT and telomerase activity effectively and induced apoptosis. It has set up a research platform for the gene therapy of tumors which take hTERT as the target.

Lentiviral vector mediated siRNA knock-down of hTERT results in diminished capacity in invasiveness and in vivo growth of human glioma cells in a telomere length-independent manner.

Glioma cells are characterized by their invasiveness and resistance against conventional therapeutics. Telomerase activity has been suggested to be an important target for glioma treatment. Here we assessed the anticancer effects and its potential mechanisms of lentiviral vector mediated siRNA knock-down of the human telomerase reverse transcriptase (hTERT) in U87MG human glioblastoma cells. Stable expression of anti-hTERT siRNA reduced the hTERT expression and TRAP assay telomerase activity to barely detectable levels. Injection of lentiviral vectors encoding anti-hTERT siRNA significantly inhibited the growth of pre-established macroscopic xenograft tumors, which was in contrast to the finding that no obvious effects on cell growth, cell cycle progression and telomere length were observed in anti-hTERT siRNA expressing U87MG cells during short-term in vitro cultures. The in vivo glioma growth inhibition effect was already evident in the period coincided with no detectable telomere length changes, suggesting that hTERT inhibition may hinder glioma cell growth in a telomere length-independent manner. Importantly, transwell migration assay showed profound inhibitory effect on the invasive capacity of U87MG cells following short-term anti-hTERT siRNA expression. Thus, efficient knock-down of hTERT can inhibit glioma cell proliferation and migration prior to its effect on telomere length.