3D Phage-based biomolecular filter for effective high throughput capture of Salmonella Typhimurium in liquid streams.

Foodborne illnesses caused by pathogens on fresh produce remain one of the most critical food safety problems the world faces. The recalls of pasta salad in 2018 and pre-cut melons in 2019 imply current methods in identifying the source of pathogens and outbreak prevention are inappropriate and time consuming. In this article, a new technology, called the 3D phage-based biomolecular filter, was developed to simultaneously capture and concentrate foodborne pathogens from large volumes of liquid streams (food liquid or wash water streams). The 3D phage-based filter consisted of phage-immobilized magnetoelastic (ME) filter elements, a filter pipe system, and a uniform magnetic field to fix and align the ME filter elements in the 3D filter column. The closely packed ME filter elements display a 3D layered structure which allows for enhanced surface interaction of the immobilized bacteriophage with specific pathogens in the passing liquid streams. As a result, a pathogen capture rate of more than 90% was achieved at a high flow rate of 3 mm/s with 20,000 ME filter elements. The capability of the 3D phage-based filter to capture pathogens in liquid streams at different filter element packing densities was further validated by experiments, finite element analysis and theoretical calculations. The capture rate increases significantly with larger numbers of ME filter elements placed in the testing pipe, and the turbulence flow induced by the 3D stacking of ME filter elements can further improve the capture efficiency. This technology enables rapid capture and analysis of large volume of water in processing fresh fruit and vegetables for the presence of small quantities of pathogens, which will ultimately benefit producers, the food industry, and society with improved food safety and production efficiency.

Role of HAND2-AS1 in human tumors.

Long noncoding RNAs (lncRNAs) are molecules more than 200 nucleotides in length. They play roles in various cells, mainly regulating cell growth, differentiation, and apoptosis. They also participate in the pathogenesis of many diseases. In fact, several studies have shown that lncRNAs function as cancer or tumor suppressor genes and play important roles in the occurrence and development of cancer in humans. New evidence has shown that lncRNA heart and neural crest derivatives expressed 2-antisense RNA 1 (lncRNA HAND2-AS1) hinders the occurrence and development of various tumors. Overexpression of HAND2-AS1 was found to be significantly related to the clinical and pathological characteristics of cancer patients, as well as the regulation of cell proliferation, apoptosis, invasion, metastasis, and energy metabolism through several possible mechanisms. Therefore, HAND2-AS1 may be a promising tumor biomarker and therapeutic target. Here, we review the biological functions, mechanisms, and potential clinical significance of HAND2-AS1 in numerous human tumors.

Magnetostrictive Microcantilever as an Advanced Transducer for Biosensors.

The magnetostrictive microcantilever (MSMC) as a high-performance transducer was introduced for the development of biosensors. The principle and characterization of MSMC are presented. The MSMC is wireless and can be easily actuated and sensed using magnetic field/signal. More importantly, the MSMC exhibits a high Q value and works well in liquid. The resonance behavior of MSMC is characterized in air at different pressures and in different liquids, respectively. It is found that the Q value of the MSMC in water reaches about 40. Although the density and viscosity of the surrounding media affect the resonance frequency and the Q value of MSMC, the density has a stronger influence on the resonance frequency and the viscosity has a stronger influence on the Q value, which result in that, for MSMC in air at pressure of less than 100 Pa, the resonance frequency of MSMC is almost independent of the pressure, while the Q value increases with decreasing pressure. MSMC array was developed and characterized. It is experimentally demonstrated that the characterization of an MSMC array is as simple as the characterization of a single MSMC. A filamentous phage against Salmonella typhimurium was utilized as bio-recognition unit to develop an MSMC based biosensor. The detection of S. typhimurium in water demonstrated that the MSMC works well in liquid.

In-air and underwater performance and finite element analysis of a flextensional device having electrostrictive poly(vinylidene fluoride-trifluoroethylene) polymers as the active driving element.

A flextensional transducer, in which the electrostrictive poly(vinylidene fluoride-trifluoroethylene) [P(VDF-TrFE)] copolymer was used as the active driving element, was fabricated and characterized. The results show that transducers of several millimeters thick can produce an axial displacement of more than 1 mm in air along the thickness direction, and a transmitting voltage response of 123 dB re 1 microPa/V at 1 m in water at frequencies of several kilohertz. A finite element code (ANSYS, Inc., Canonsburg, PA) was used to model the in-air and underwater responses of the flextensional transducer over a broad frequency range. The calculated resonance frequencies and transmitting voltage response spectra show good agreement with the experimental data. In addition, the performance of both the in-air actuator and underwater transducer was analyzed for different design parameters of the flextensional structure. These results show that the performance of the flextensional transducer could be tailored readily by adjusting the parameters of the flextensional metal shell.

Design, fabrication, and performance of a flextensional transducer based on electrostrictive polyvinylidene fluoride-trifluoroethylene copolymer.

Taking advantage of the high electrostrictive strain and high elastic energy density of a newly developed electrostrictive polymer, modified poly(vinylidene fluoride-trifluoroethylene) [P(VDF-TrFE)] based polymers, a flex-tensional transducer was designed, and its performance was investigated. The flextensional transducer consists of a multilayer stack made of electrostrictive P(VDF-TrFE) polymer films and two flextensional shells fixed at the ends to the multilayer stack. Because of the large transverse strain level achievable in the electrostrictive polymer and the displacement amplification of the flextensional shells, a device of a few millimeters thick and lateral dimension about 30 mm x 25 mm can generate an axial displacement output of more than 1 mm. The unique flextensional configuration and the high elastic energy density of the active polymer also enable the device to offer high-load capability. As an underwater transducer, the device can be operated at frequencies below 1 kHz and still exhibit relatively high transmitting voltage response (TVR), very high source level (SL), and low mechanical quality factor (Qm).