Bioinformatics and biomedical informatics with ChatGPT: Year one review.

The year 2023 marked a significant surge in the exploration of applying large language model (LLM) chatbots, notably ChatGPT, across various disciplines. We surveyed the applications of ChatGPT in bioinformatics and biomedical informatics throughout the year, covering omics, genetics, biomedical text mining, drug discovery, biomedical image understanding, bioinformatics programming, and bioinformatics education. Our survey delineates the current strengths and limitations of this chatbot in bioinformatics and offers insights into potential avenues for future developments.

Equivalent chemical substitution in double-double perovskite-type ALaLiTeO6:Mn4+ (A = Ba2+, Sr2+, Ca2+) phosphors enabling wide range crystal field strength regulation and efficient far-red emission.

Mn4+-activated phosphors have shown wide prospective applications in phosphor-converted white light-emitting diodes (pc-WLEDs) and pc-LEDs used in illumination and indoor plant cultivation, respectively. Recently, double perovskites A2B'B''O6 with a tunable crystal structure and versatile octahedral sites have been extensively studied as good host matrixes for Mn4+-emitters to realize tunable far-red emissions. Herein, a series of double-double perovskite-type ALaLiTeO6:Mn4+ (A = Ba, Ba0.5Sr0.5, Sr, Sr0.5Ca0.5, Ca) phosphors were synthesized and structurally characterized, and the correlations between their structure and luminescence were also studied systematically. With a decrease of the A-cation size, an increased distortion in the average structure and a structure symmetry lowering (I2/m → P21/n) were observed for ALaLiTeO6:Mn4+. In contrast, on the local scale, the degree of (Li/Te)O6-octahedral distortion is positively correlated with the ΔIR value, which is the ionic radius difference between A2+ and La3+. The local structural changes were found to be irrelevant to the significant improvements in photoluminescence properties. In combination with careful spectroscopic analysis, we deciphered that a decreased A-cation is in fact helpful for the enhancements in crystal field strength (Dq/B = 2.12-2.82) and Mn-O covalent bonding, thereby resulting in an improved quantum efficiency, a suppressed nonradiative transition, and a redshift in photoluminescence spectra. Amongst the ALaLiTeO6:Mn4+ phosphor series, CaLaLiTeO6:Mn4+ exhibits the highest external quantum efficiency of 70.1% and internal quantum efficiency of 96.4% and superior thermal stability (93.3%@423 K), making CaLaLiTeO6:Mn4+ very promising as far-red phosphors for pc-LEDs. The findings of this work will serve as a new guide for rational design of high-performance Mn4+-activated double-double perovskite-type far-red phosphors.

Description of Nilgiriuspygoprominulus sp. n. (Opiliones, Assamiidae, Trionyxellinae) from China, with notes on its sexual dimorphism.

Background: A new species of Nilgirius, N.pygoprominulus sp. n. (male and female) in the family Assamiidae from Yunnan Province, China, is described and illustrated herein. Sexual size dimorphism (male larger than female) is inconsistent with most assamiids. Other sexually dimorphic features (body shape, leg IV and pseudonychium) are reported. New information: Nilgiriuspygoprominulus sp. n. is described as a new species of Trionyxellinae. Information about sexual dimorphism of the species is reported.

Development and verification of multiplex real-time RT-PCR assay for classing and identification of HPIV1,HPIV2 and HPIV3 / 中国生物制品学杂志

@#ObjectiveTo develop and verify a multiplex real-time RT-PCR assay for simultaneous identification of human parainfluenza virus type 1(HPIV1),type 2(HPIV2)and type 3(HPIV3).MethodsThe whole genome sequences of HPIV1,HPIV2 and HPIV3 were downloaded from the database for alignment analysis,and the conserved regions were selected. Specific primers and probes were designed for the three viruses respectively to develop a multiplex real-time RTPCR assay with human ribonuclease P(RNase P)as theinternal quality control. The method was verified for the sensitivity,specificity and precision and used to detect 192 clinical samples.ResultsAfter optimization,the multiplex real-time RTPCR reaction system was determined to be 30 μL,including 10 × NeoscriptRTase/UNG Multi mix 3 μL,5 × Neoscript RT Premix Multi Buffer 6 μL,upstream and downstream primers of HPIV1,HPIV2 and HPIV3(100 μmol/L)0. 1 μL respectively,HPIV1,HPIV2,HPIV3 probes(100 μmol/L)0. 05 μL respectively,RNase P upstream and downstream primers(50 μmol/L)0. 06 μL respectively,RNase P probe(50 μmol/L)0. 03 μL respectively,template 15 μL,and ddH2O supplemented to 30 μL. The reaction conditions were 50 ℃ 20 min,95 ℃ 3 min and 45 cycles of 95 ℃ 15 s and54 ℃ 30 s. Fluorescence signals were collected during annealing in each cycle. The minimum detection limits of HPIV1,HPIV2 and HPIV3 were all 500 copies/mL by the multiplex real-time RT-PCR assay;The method showed no cross-reaction with influenza A virus,influenza B virus,respiratory syncytial virus andnovel coronaviruses. The coefficients of variation(CVs)in intra-and inter-groups of the recombinant plasmid standard mixture with three different concentrations were all less than 3%. HPIV1,HPIV2 and HPIV3 were detected in 192 clinical samples,and the positive rates were7. 81%,0. 05% and 3. 1%,respectively.ConclusionThe multiplex real-time RT-PCR assay for detection of HPIV1,HPIV2 and HPIV3 developed in this study has good sensitivity,specificity and precision,which has a high clinical application prospect in the field of rapid diagnosis and identification of HPIV.

Enhancing the oxide-ionic conductivity of Ba3Mo1+xNb1-2xGexO8.5 at intermediate temperatures: the effect of site-selective Ge4+-substitution.

Significant oxide-ionic conductivity has been recently reported for a family of cation-deficient hexagonal perovskite derivatives Ba3M2O8.5 (M = Mo/W6+ and Nb5+/V5+). Herein, strong 4-fold coordination geometry preferring Ge4+ ions are doped into Ba3Mo1+xNb1-2xGexO8.5 to manipulate the oxygen distribution within palmierite-like layers for the enhancement of oxide-ionic conductivity. Rietveld refinement of the neutron diffraction data of Ba3Mo1.2Nb0.6Ge0.2O8.5 reveals that Ge4+-ions are selectively incorporated into the palmierite-like layers, owing to their strong 4-fold coordination environment preference. Such a site-selective doping behavior leads to an increase in the occupation proportion of the O3 site and a concomitant decrease in the occupancy factor for O2. Ionic conduction measurements show that the bulk conductivity of Ba3Mo1.2Nb0.6Ge0.2O8.5 is about twice higher than that of the parent compound at intermediate temperatures (300-500 °C). Furthermore, bond-valence site energy (BVSE) landscape analysis reveals that the oxygen ionic conduction of Ba3Mo1+xNb1-2xGexO8.5 is dominated by the two-dimensional pathways along the palmierite-like layers, despite the three-dimensional (3D) oxygen diffusion pathways being present in the hybrid structure, which strongly confirms that the enhancement in ionic conductivity at intermediate temperatures is attributed to the site-selective Ge4+-substitution-induced redistribution of oxygen ions within the palmierite-like layers.