ABSTRACT
A new oleanane-type triterpene, ardisiapunine A (1), together with eight known compounds were isolated from the roots of Ardisia lindleyana D.Dietr. Their chemical structures were determined by means of spectroscopic methods including HR-ESI-MS and (1 D, 2 D) NMR data. The absolute configuration of compound 1 was established by a single-crystal X-ray diffraction experiment. The new compound is an unusual oleanane-type triterpene bearing an acetal and a C-13-C-18 double bond. The cytotoxicity of all isolated compounds were evaluated using four human cancer cell lines, including A549, HepG2, HeLa and U87. The new compound 1 and compound 2 were weakly active but the known compound 6 exhibited a high cytotoxicity compared to cisplatin.
Subject(s)
Antineoplastic Agents, Phytogenic , Antineoplastic Agents , Ardisia , Saponins , Triterpenes , Humans , Ardisia/chemistry , Triterpenes/pharmacology , Triterpenes/chemistry , Molecular Structure , HeLa Cells , Antineoplastic Agents, Phytogenic/pharmacology , Antineoplastic Agents, Phytogenic/chemistry , Saponins/chemistryABSTRACT
Objective To design and synthesze novel pyrrolopyridine as integrase inhibitors. Method The launched anti-HIV drug raltegravir was selected as template compounds. According to the concept of bioisosterism and molecular docking ,a series of pyrrolopyridine compounds(6a-6t)were designed,synthesized and their anti-integrase 3′-processing activity were analyzed. Re?sults The designed compounds were successfully synthesized and the structures of these compounds were confirmed by 1H NMR,13C NMR and ESI-HRMS. The anti-IN 3′-P activity of these compounds were also measured. The binding mode and docking energy of rep?resentative compounds were analyzed. Conclusion The structure-activity relationships of these compounds were also analyzed by the results of docking. These results lay the foundation for the further optimization of these compounds.
