Single-cell data analysis of malignant epithelial cell heterogeneity in lung adenocarcinoma for patient classification and prognosis prediction.

Lung cancer is one of the leading causes of cancer-related death. Most advanced lung adenocarcinoma (LUAD) patients have poor survival because of drug resistance and relapse. Neglecting intratumoral heterogeneity might be one of the reasons for treatment insensitivity, while single-cell RNA sequencing (scRNA-seq) technologies can provide transcriptome information at the single-cell level. Herein, we combined scRNA-seq and bulk RNA-seq data of LUAD and identified a novel cluster of malignant epithelial cells - KRT81+ malignant epithelial cells - associated with worse prognoses. Further analysis revealed that the hypoxia and EMT pathways of these cells were activated to predispose them to differentiate into metastatic lung adenocarcinoma cells. Finally, we also studied the role of these tumor cells in the immune microenvironment and their role in the classification and prognosis prediction of lung adenocarcinoma patients.

Pathological changes and outcomes of coronary artery lesions in Kawasaki disease / 中华实用儿科临床杂志

Kawasaki disease is a nonspecific systemic vasculitis of unknown etiology which mainly involves small and medium arteries.The most serious complication is coronary artery lesions, which can lead to serious cardiovascular events.Because of the unknown etiology and pathogenesis, the acute pathological changes and long-term evolution of coronary artery lesions are particularly important for clinical diagnosis, treatment and long-term management.This article reviews the research progress of coronary artery pathology in Kawasaki disease and the long-term prognosis of coronary artery lesions.