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13.
Pharm Res ; 36(6): 88, 2019 04 19.
Article in English | MEDLINE | ID: mdl-31004233

ABSTRACT

In the original manuscript, the Figure 2 a-i is inadvertently repeated as Figure 2 a-ii. This mistake has been rectified and the corrected Figure 2 is presented below.

16.
RSC Adv ; 9(27): 15571-15572, 2019 May 14.
Article in English | MEDLINE | ID: mdl-35532484

ABSTRACT

[This corrects the article DOI: 10.1039/C4RA11969J.].

17.
J Biomed Nanotechnol ; 11(3): 392-402, 2015 Mar.
Article in English | MEDLINE | ID: mdl-26307823

ABSTRACT

This study aims at the targeted delivery of 5-fluorouracil (5-FU) and Megestrol acetate (Meg) loaded fibrinogen-graft-poly(N-Vinyl caprolactam) nanogels (5-FU/Meg-fib-graft-PNVCL NGs) toward α5ß1-integrins receptors expressed on breast cancer cells to have enhanced anti-cancer effect in vitro. To achieve this aim, we developed biocompatible thermoresponsive fib-graft-PNVCL NGs using fibrinogen and carboxyl terminated PNVCL via EDC/NHS amidation reaction. The Lower Critical Solution Temperature (LCST) of fib-graft-PNVCL could be tuned according to PNVCL/fibrinogen compositions. The 100-120 nm sized nanogels of fib-graft-PNVCL (LCST = 35 ?1 'C) was prepared using CaCl2 cross-linker. The 5-FU/Meg-fib-graft-PNVCL NGs showed a particle size of 150-170 nm size. The drug loading efficiency with 5-FU was 62% while Meg showed 74%. The 5-FU and Meg release was prominent above LCST than below LCST. The multi drug loaded fib-graft-PNVCL NGs showed enhanced toxicity, apoptosis and uptake by breast cancer (MCF-7) cells compared to their individual doses above their LCST. The in vivo assessment in Swiss albino mice showed sustained release of Meg and 5-FU as early as 3 days, confirming the therapeutic efficiency of the formulation. These results demonstrate an enhanced platform for the future animal studies on breast tumor xenograft model.


Subject(s)
Antineoplastic Combined Chemotherapy Protocols/administration & dosage , Breast Neoplasms/drug therapy , Caprolactam/analogs & derivatives , Delayed-Action Preparations/administration & dosage , Fibrinogen/pharmacokinetics , Nanocapsules/administration & dosage , Polymers/chemistry , Antineoplastic Combined Chemotherapy Protocols/chemistry , Breast Neoplasms/metabolism , Breast Neoplasms/pathology , Caprolactam/chemistry , Cell Line, Tumor , Cell Survival/drug effects , Delayed-Action Preparations/chemical synthesis , Diffusion , Fibrinogen/chemistry , Fluorouracil/administration & dosage , Fluorouracil/chemistry , Humans , Hydrogels/chemical synthesis , Megestrol Acetate/administration & dosage , Megestrol Acetate/chemistry , Nanocapsules/chemistry , Nanocapsules/ultrastructure , Particle Size , Receptors, Vitronectin/metabolism , Temperature
18.
Tissue Eng Part C Methods ; 21(10): 1044-58, 2015 Oct.
Article in English | MEDLINE | ID: mdl-25962715

ABSTRACT

The dental follicle is a source of dental follicle stem cells (DFCs), which have the potential to differentiate into the periodontal lineage. DFCs therefore are of value in dental tissue engineering. The purpose of this study was to evaluate the effect of growth factor type and concentration on DFC differentiation into periodontal specific lineages. DFCs were isolated from the human dental follicle and characterized for the expression of mesenchymal markers. The cells were positive for CD-73, CD-44, and CD-90; and negative for CD-33, CD-34, and CD-45. The expression of CD-29 and CD-31 was almost negligible. The cells also expressed periodontal ligament and cementum markers such as periodontal ligament-associated protein-1 (PLAP-1), fibroblast growth factor-2 (FGF-2), and cementum protein-1 (CEMP-1), however, the expression of osteoblast markers was absent. Further, the DFCs were cultured in three different induction medium to analyze the osteoblastic, fibroblastic, and cementoblastic differentiation. Runt-related transcription factor 2 (RUNX-2), alkaline phosphatase (ALP) activity, alizarin staining, calcium quantification, collagen type-1 (Col-1), and osteopontin (OPN) expression confirmed the osteoblastic differentiation of DFCs. DFCs cultured in recombinant human FGF-2 (rhFGF-2) containing medium showed enhanced PLAP-1, FGF-2, and COL-1 expression with increasing concentration of rhFGF-2 which thereby confirmed periodontal ligament fibroblastic differentiation. Similarly, DFCs cultured in recombinant human cementum protein-1 (rhCEMP-1) containing medium showed enhanced bone sialoprotein-2 (BSP-2), CEMP-1, and COL-1 expression with respect to rhCEMP-1 which confirmed cementoblastic differentiation. The expression of osteoblast, fibroblast, and cementoblast-related genes of DFCs cultured in induction medium was enhanced in comparison to DFCs cultured in noninduction medium. Thus, growth factor-dependent differentiation of DFCs into periodontal specific lineages was proved by quantitative analysis.


Subject(s)
Cell Differentiation , Dental Cementum/metabolism , Dental Sac/metabolism , Fibroblasts/metabolism , Osteoblasts/metabolism , Periodontium/metabolism , Stem Cells/metabolism , Adolescent , Adult , Dental Cementum/cytology , Dental Sac/cytology , Female , Fibroblasts/cytology , Humans , Male , Organ Specificity , Osteoblasts/cytology , Periodontium/cytology , Stem Cells/cytology
19.
ACS Appl Mater Interfaces ; 7(18): 9399-409, 2015 May 13.
Article in English | MEDLINE | ID: mdl-25893690

ABSTRACT

Injectable gel systems, for the purpose of bone defect reconstruction, have many advantages, such as controlled flowability, adaptability to the defect site, and increased handling properties when compared to the conventionally used autologous graft, scaffolds, hydroxyapatite blocks, etc. In this work, nanohydroxyapatite (nHAp) incorporated chitin-poly(ε-caprolactone) (PCL) based injectable composite microgels has been developed by a simple regeneration technique for bone defect repair. The prepared microgel systems were characterized using scanning electron microscope (SEM), Fourier transformed infrared spectroscopy (FTIR), and X-ray diffraction (XRD). The composite microgel, with the incorporation of nHAp, showed an increased elastic modulus and thermal stability and had shear-thinning behavior proving the injectability of the system. The protein adsorption, cytocompatibility, and migration of rabbit adipose derived mesenchymal stem cells (rASCs) were also studied. Chitin-PCL-nHAp microgel elicited an early osteogenic differentiation compared to control gel. The immunofluorescence studies confirmed the elevated expression of osteogenic-specific markers such as alkaline phosphatase, osteopontin, and osteocalcin in chitin-PCL-nHAp microgels. Thus, chitin-PCL-nHAp microgel could be a promising injectable system for regeneration of bone defects which are, even in deeper planes, irregularly shaped and complex in nature.


Subject(s)
Bone Regeneration/drug effects , Bone and Bones/physiology , Chitin/pharmacology , Durapatite/pharmacology , Gels/chemistry , Nanoparticles/chemistry , Polyesters/pharmacology , Tissue Engineering/methods , Adipose Tissue/cytology , Adsorption , Alkaline Phosphatase/metabolism , Animals , Blood Proteins/metabolism , Bone and Bones/drug effects , Cell Adhesion/drug effects , Cell Differentiation/drug effects , Cell Movement/drug effects , Elasticity/drug effects , Immunohistochemistry , Injections , Mesenchymal Stem Cells/cytology , Mesenchymal Stem Cells/drug effects , Osteocalcin/metabolism , Osteopontin/metabolism , Rabbits , Rheology/drug effects , Temperature , Viscosity/drug effects
20.
Colloids Surf B Biointerfaces ; 127: 105-13, 2015 Mar 01.
Article in English | MEDLINE | ID: mdl-25660093

ABSTRACT

Reduced levels of endogenous growth factors and diminished angiogenesis are contributory factors for impaired wound healing in diabetic patients. Vascular endothelial growth factor (VEGF) is the most potent angiogenic growth factor which stimulates multiple phases of wound healing angiogenesis and thereby accelerates healing. The aim of this work was to develop chitosan-hyaluronic acid composite sponge incorporated with fibrin nanoparticles loaded with VEGF as a wound dressing for diabetic wounds. VEGF loaded fibrin nanoparticles (150-180 nm) were prepared and characterized which were then incorporated to the composite sponge. The prepared sponges were characterized by SEM and FT-IR. Porosity, swelling, biodegradation, mechanical properties and haemostatic potential of the sponges were also studied. Release of VEGF from the composite sponges was evaluated using ELISA kit. More than 60% of the loaded VEGF was released in three days. Cell viability and attachment studies of the composite sponges were evaluated using human dermal fibroblast (HDF) cells and human umbilical vein endothelial cells (HUVECs). HUVECs seeded on VEGF containing sponges showed capillary like tube formation which was absent in control sponges. The results suggest that the prepared chitosan-hyaluronic acid/VEGF loaded nanofibrin composite sponges (CHVFS) have potential to induce angiogenesis in wound healing.


Subject(s)
Bandages , Chitosan/pharmacology , Fibrin/pharmacology , Hyaluronic Acid/pharmacology , Nanoparticles/chemistry , Neovascularization, Physiologic/drug effects , Vascular Endothelial Growth Factor A/pharmacology , Wound Healing/drug effects , Blood Coagulation/drug effects , Cell Adhesion/drug effects , Cell Proliferation/drug effects , Cell Survival/drug effects , Human Umbilical Vein Endothelial Cells , Humans , Kinetics , Materials Testing , Microscopy, Fluorescence , Nanocomposites/chemistry , Nanocomposites/ultrastructure , Nanoparticles/ultrastructure , Porosity
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