1.
Bioorg Khim
; 18(2): 210-6, 1992 Feb.
Article
in Russian
| MEDLINE
| ID: mdl-1534987
ABSTRACT
In search for sorbents (silica gels, styrene-divinylbenzene copolymers), for immobilization of some restriction endonucleases, derivatives of trityl-containing silochroms are shown to bind EcoRI, PaeI and LplI endonucleases with the retention of 10-20, 60-70 and 40-60% activity, respectively. The immobilized restriction endonucleases have the unchanged substrate specificity, can be used several times and are stable at storage. Tritylaminopropylsilochrom is suggested to be the sorbent of choice.
Subject(s)
Deoxyribonuclease EcoRI/metabolism , Deoxyribonucleases, Type II Site-Specific/metabolism , Enzymes, Immobilized , Bacteriophage lambda/metabolism , DNA, Viral/metabolism
2.
Zh Mikrobiol Epidemiol Immunobiol
; (8): 65-7, 1991 Aug.
Article
in Russian
| MEDLINE
| ID: mdl-1950290
Subject(s)
Antigens/blood , Erythrocytes/immunology , Immunosorbent Techniques , Adsorption , Dose-Response Relationship, Immunologic , Humans , Hydrogen-Ion Concentration , Immunosorbent Techniques/instrumentation , Immunosorbent Techniques/statistics & numerical data , Polystyrenes , Sensitivity and Specificity , Serum Albumin/immunology , Surface Properties , Temperature
3.
Dokl Akad Nauk SSSR
; 302(6): 1511-3, 1988.
Article
in Russian
| MEDLINE
| ID: mdl-2466629