ABSTRACT
The study of the activity of natural killer cells in patients with chronic alcoholism, stage II, has revealed its essential decrease in comparison with that in healthy subjects, which is particularly pronounced with effector/target cell ratio equal to 100:1 and 50:1 respectively. Besides, two subgroups can be differentiated among the total population of alcoholic patients: subgroup 1 with the activity of killer cells remaining practically intact and subgroup 2 with profound defects in the functioning of these cells. The presence of essential defects in natural killer cells of patients with the severe form of alcoholism is suggested.
Subject(s)
Alcoholism/immunology , Killer Cells, Natural/immunology , Adult , Cytotoxicity Tests, Immunologic , Ethanol/adverse effects , Humans , Immunity, Cellular , Substance Withdrawal Syndrome/immunologyABSTRACT
The data of the study of alpha/beta interferon (IFN) effect in mice of different genotype were presented. CBA mice of H-2k genotype, C57B1/6 mice of H-2b genotype and their hybrid (CBA X C57B1/6) F1 have been used in the experiments. IFN has been injected intraperitoneally in a dose of 100-5000 U/mouse in combination with antigenic stimulation. It was shown that IFN enhanced stem cells migration from bone marrow in CBA, but not in (CBA X C57B1/6)F1 mice. At the same time the splenocytes from CBA mice were more sensitive to inhibition by IFN than splenocytes from C57B1/6 mice. This was found in antibody and immune rosette-formation tests. The effect of IFN on the immune system cells is probably predetermined by the individual genetic characteristics of a mouse strain.
Subject(s)
Adjuvants, Immunologic/genetics , Interferon Type I/genetics , Adjuvants, Immunologic/immunology , Animals , Antibody-Producing Cells/drug effects , Cell Movement/drug effects , Hematopoietic Stem Cells/drug effects , Hybridization, Genetic , Interferon Type I/immunology , Mice , Mice, Inbred C57BL/genetics , Mice, Inbred CBA/genetics , Rosette FormationABSTRACT
A study of immunomodulating action of mouse alpha/beta interferon (IFN) was performed in conditions of congenital and experimental thymus-dependent immune deficiency. The action of IFN was assessed in vivo, with IFN injected intraperitoneally. It was shown that IFN did not change killer effect of lymphocytes in the reaction of stem cell inactivation in "nude" mice, but enhanced antibody and rosette formation in the spleen of these animals. In addition, IFN did not change these parameters in the spleen of HRS mice with abnormal differentiation of T lymphocytes, but enhanced antibody and rosette formation in the spleen of mice, thymectomized in old age. It is concluded that the normal function of T-system is essential for the action of IFN.
Subject(s)
Adjuvants, Immunologic , Immunologic Deficiency Syndromes/congenital , Immunologic Deficiency Syndromes/therapy , Interferon Type I/therapeutic use , Thymus Gland/immunology , Animals , Antibody Formation/drug effects , Hematopoietic Stem Cells/drug effects , Hematopoietic Stem Cells/immunology , Immunologic Deficiency Syndromes/immunology , Male , Mice , Mice, Inbred Strains , Rosette Formation , T-Lymphocytes/drug effects , T-Lymphocytes/immunology , Thymus Gland/drug effectsSubject(s)
Thymus Gland/immunology , Animals , Bone Marrow Transplantation , Interleukin-2/immunology , Killer Cells, Natural/immunology , Lymphatic Diseases/immunology , Lymphatic Diseases/therapy , Mice , Mice, Nude , Radiation Chimera , Rosette Formation , T-Lymphocytes/immunology , T-Lymphocytes/transplantation , Thymectomy , Transplantation, IsogeneicABSTRACT
Thymosine, a thymus hormone, restores the thymectomy induced deterioration of the routine pathways of migration and differentiation ofhemopoietic stem cells in mice. Administration of thymosine together with bone marrow cells from thymectomized mice to irradiated recipients also restores the level of migration and differentiation of hemopoietic stem cells. The inducing effect of thymosine on the maturation of T-lymphocyte precursors, which in their turn restore the usual rate of migration and differentiation of hemopoietic stem cells, has been suggested.
Subject(s)
Hematopoietic Stem Cells/drug effects , Thymosin/pharmacology , Thymus Hormones/pharmacology , Animals , Bone Marrow/radiation effects , Cell Differentiation/drug effects , Cell Movement/drug effects , Colony-Forming Units Assay , Gamma Rays , Mice , Mice, Inbred C57BL , Mice, Inbred CBA , ThymectomyABSTRACT
Lymph node cells of normal CBA mice, syngeneic radiation chimerae CBA leads to CBA and B-mice after incubation with thymosin (fraction 5) were transplanted to sublethally irradiated recipients (CBA X C57BL) F1; the number of endogenic colonies in the recipient's spleen was then recorded. Thymosin was shown to increase the killer activity of the lymph node cells of normal mice CBA, syngeneic chimerae CBA leads to CBA, but not of B-mice. As suggested, TU-cells' subpopulation served as target cells for thymosin.
