ABSTRACT
The interaction between milk xanthine oxidase (XO) and lactoperoxidase (LP) in model system and antimicrobial action of these enzymes on Escherichia coli 0-111 were studied. It was shown, that bacterial superoxide dismutase (SOD), which transforms O2-. (XO-reaction product) into H2O2 (substrate of LP), is necessary for binding of the reaction sequence: XO-->LP-->antimicrobial products. It is suggested, that these enzymes unite in the protective system in intestinal infections of newborns. Bacterial SOD in this case acts as the key factor, creating the system.
Subject(s)
Escherichia coli/immunology , Lactoperoxidase/immunology , Milk/enzymology , Superoxide Dismutase/immunology , Xanthine Oxidase/immunology , Animals , Cattle , Escherichia coli/enzymology , Free Radicals , Milk/immunologyABSTRACT
The adsorption activity of formolated sheep red blood cells can be increased by their combined treatment with low-frequency ultrasound, tannin and Tween-40. The diagnosticums thus obtained have proved to be highly sensitive and specific and can be recommended for the detection of antibodies to Bordetella parapertussis in the sera of sick children.
Subject(s)
Bordetella/immunology , Erythrocytes/immunology , Animals , Antibodies, Bacterial/analysis , Antigens, Bacterial/immunology , Bordetella pertussis/immunology , Child , Erythrocytes/drug effects , Hemagglutination Inhibition Tests , Hemagglutination Tests , Humans , Polysorbates/pharmacology , Sheep/immunology , Tannins/pharmacology , UltrasonicsABSTRACT
The authors elaborated a method of obtaining pertussis soluble antigenic complex by dialysis through the cellophane membrane against the physiological saline at a temperature of 4 degrees C. An antigen which was active in the passive hemagglutination and neutralization of antibodies tests was revealed in the dialyzate. The amount of this antigen in the dialyzate increased gradually up to the 7th day and then became stabilized. The serological activity of the antigen after evaportation increased 4-16 times. The results of the antibody neutralization test pointed to the presence in the dialysate of substances common to those contained in the 1a and 1Da fractions isolated from the pertussis bacteria with the aid of ammounium sulfate.
