ABSTRACT
In experiment on 12 Chinchilla rabbits dynamics of reparative regeneration was studied at the terms 2 and 4 months. Bone defect in mandible corner was closed by osteoplastic material Gapkol which was covered from inside by allogenic or autologic stem cells received from rabbit adipose tissue. The results of the ray tracing methods of study were verified by SEM and histological methods.
Subject(s)
Bone Regeneration , Collagen , Dental Materials , Durapatite , Mandible/surgery , Mesenchymal Stem Cell Transplantation , Osteogenesis , Adipose Tissue , Animals , Drug Combinations , Follow-Up Studies , Mesenchymal Stem Cells/cytology , Microscopy, Electron, Scanning , Osteosclerosis/surgery , Rabbits , Time Factors , Tomography, X-Ray Computed , Transplantation, Autologous , Transplantation, HomologousABSTRACT
Reconstructive surgery often collides with complicated task of defect recovery in the situation of deficit of maxillofacial skeleton tissues, that leads to necessary search of accessible cells source with osteogenic potentials and proper substrate for cell transplantation. In the study the regeneratory potential of stromal cells of adipose tissue was tested in the rabbit model of mandible through angle defect. Cells of allogenic and autologic nature stimulated reparative osteogenesis but their influence upon bone matrix maturation was different. When the construction with allogenic cells was transplanted reaction of its rejection was not detected on histological level.
Subject(s)
Adipose Tissue/cytology , Bone Regeneration , Mandible/surgery , Multipotent Stem Cells , Osteogenesis , Stem Cell Transplantation , Stromal Cells , Animals , Bone Matrix/physiology , Cell Differentiation , Follow-Up Studies , Mandible/physiology , Multipotent Stem Cells/physiology , Multipotent Stem Cells/transplantation , Osteogenesis/physiology , Rabbits , Stromal Cells/physiology , Stromal Cells/transplantation , Time Factors , Tissue Engineering , Transplantation, Autologous , Transplantation, HomologousABSTRACT
Digital microfocal x-ray study was experimentally studied in animals to examine the time course of changes in their bone regeneration. Sixteen Chinchila rabbits whose bone defect in the angle of the mandibular ramus had been closed with the osteoplastic material Gapcol with the applied allogeneic, autologous stem cells isolated from rabbit adipose tissue and human plasma enriched with thrombocytic growth factors were examined. The capabilities of digital microfocal x-ray study versus x-ray computed tomography were compared in the evaluation of reparative regeneration of bone tissue. The results of radiation studies were verified with the data of scanning electron microscopy.
