ABSTRACT
An increase in frequency of heart contractions, changes in the bioelectrical heart activity and decrease of the force-pump heart function are observed in the rabbits with model chronic ischemic heart disease lipid metabolism in significantly changed the concentration of cholesterol in the right ventricle myocardium as well as the concentrations of unsaturated lipid acids in the heart tissues increase, while the content of saturated lipid acids and antioxidant activity of plasma decrease. Bioenergetic processes change as follows: succinate-dehydrogenase and alpha-keto-glutarate-dehydrogenase activity decrease, while the concentrations of glycogen, lactic and pyruvic acids and lactate dehydrogenase activity in the heart tissues increase.
Subject(s)
Cholesterol, Dietary/administration & dosage , Myocardial Ischemia/metabolism , Animals , Cholesterol/metabolism , Chronic Disease , Disease Models, Animal , Glycogen/metabolism , Ketoglutarate Dehydrogenase Complex/metabolism , L-Lactate Dehydrogenase/metabolism , Lactates/metabolism , Lactic Acid , Lipid Metabolism , Myocardial Contraction/physiology , Myocardial Ischemia/chemically induced , Oxidation-Reduction , Pyruvates/metabolism , Pyruvic Acid , Rabbits , Succinate Dehydrogenase/metabolismABSTRACT
While laboratory experimental model of coronary heart disease (according to Frol'kis et al.) is developed, activity of succinate dehydrogenase, alpha-ketoglutarate dehydrogenase, Na+, Ka(+)- and Mg2+ ATPase decreases, but activity of lactate dehydrogenase and concentrations of lactic and pyruvic acids in the heart tissue increase. At the same time concentration of glycogene increases more than twice. As far as we can see there is an evidence of a decrease of glycogene utilization due to change in levels of regulatory processes. Despite a decrease of ATP synthesis by the inhibition of tricarboxylic acid cycle the ATP:ADP relation reduces to ATP, as emphatic inhibition of ATPase in the heart tissues takes place in development of the model of the coronary heart disease. The relation between ATP and ADP is considered as a regulator of glycogene utilization. In the liver tissue activity of succinate dehydrogenase, alpha-ketoglutarate dehydrogenase, Na+, K(+)- and Mg2+ ATPase falls, while concentrations of lactic acid grow. No accumulation of glycogen is observed. It is obvious that there are controversial metabolic processes. Experimental data are discussed.
Subject(s)
Glycogen/metabolism , Liver Glycogen/metabolism , Liver/metabolism , Myocardial Ischemia/metabolism , Myocardium/metabolism , Animals , Chronic Disease , Disease Models, Animal , Energy Metabolism , Glycogen/analysis , Liver/chemistry , Liver Glycogen/analysis , Myocardium/chemistry , Oxidation-Reduction , RabbitsABSTRACT
The irritating, sensitizing, and toxic effect of water produced by the method of carbon dioxide hydration was examined in animal experiments and human observations. It was found that before and after decontamination this water did not cause skin irritation or organism sensitization, did not have a toxic effect on the CNS, liver, kidneys or blood morphology, and did not deteriorate adaptive capabilities of the animal or human body.
Subject(s)
Aerospace Medicine , Carbon Dioxide/chemical synthesis , Ecological Systems, Closed , Hydrogen Bonding , Nickel/toxicity , Space Flight , Water Pollutants, Chemical/toxicity , Water Supply/standards , Animals , Guinea Pigs , Humans , Nickel/isolation & purification , Rats , USSR , Water Pollutants, Chemical/isolation & purification , Water Supply/analysisSubject(s)
Fatty Acids/classification , Lipopolysaccharides/classification , Proteus/classification , Chromatography, Gas , Fatty Acids/analysis , Gas Chromatography-Mass Spectrometry , Lipopolysaccharides/analysis , Proteus/analysis , Proteus mirabilis/analysis , Proteus mirabilis/classification , Proteus vulgaris/analysis , Proteus vulgaris/classificationABSTRACT
The comparative study of the fatty acid composition of bacterial lipopolysaccharides (LPS) in the genera Escherichia, Shigella, Salmonella has been carried out under identical experimental conditions. The LPS of the bacteria under study have been found to contain a number of saturated fatty acids and oxyacids which could not be previously detected in these bacteria in other studies. In all bacterial strains under study LPS include mainly 3-oxytetradecanoic, tetradecanoic and dodecanoic fatty acids. The essential feature of the fatty acid composition of Salmonella is the presence of 2-oxytetradecanoic acid; this acid is absent in Escherichia and Shigella, which can thus be used as a differentiating criterion. The content of other fatty acids in Salmonella is similar to that in Escherichia and Shigella. These data confirm that the genera Escherichia, Shigella and Salmonella are phylogenetically related, the relationship between Escherichia and Shigella being more close.
Subject(s)
Escherichia/classification , Fatty Acids/analysis , Lipopolysaccharides/analysis , Salmonella/classification , Shigella/classification , Chromatography, Gas , Escherichia/analysis , Fatty Acids/classification , Lipopolysaccharides/classification , Salmonella/analysis , Shigella/analysisABSTRACT
The fatty acid composition of total cellular lipids and lipid A of lipopolysaccharides was studied in Providencia alcalifaciens and P. stuartii. Significant differences were found in the fatty acid composition of total cellular lipids from the above species; these are caused mainly by the presence of cyclopropane fatty acids (methylenehexadecanoic and methyleneoctadecanoic acids) in P. stuartii and their absence in P. alcalifaciens. Differences were also established in the content of fatty acids in lipid A of lipopolysaccharides from P. alcalifaciens and P. stuartii. The data are consistent with the idea that P. alcalifaciens and P. stuartii are separate taxons though, apparently, closely related.
