ABSTRACT
It has been shown that most of Drosophila melanogaster mutant lines obtained as a result of X-rays irradiation (XI) as well as of the combined action of XI and some chemical agents are characterized by decreased indexes of average (7-40 %) and maximal (1-35 %) life span. Insertion-excision processes at the instable genes white and cut are among the reasons of decreased vitality and shortened life span in induced mutants. Collection of neurodegenerative mutants has been obtained under the influence of ENU. Fast dying of flies and decreased vitality correlated with time point of neurodegenerations in brain structure.
Subject(s)
Drosophila melanogaster , Longevity , Mutation , Animals , Brain/drug effects , Brain/pathology , Brain/radiation effects , Caffeine/toxicity , Drosophila melanogaster/drug effects , Drosophila melanogaster/genetics , Drosophila melanogaster/growth & development , Drosophila melanogaster/radiation effects , Ethylnitrosourea/toxicity , Genes, Insect/drug effects , Genes, Insect/radiation effects , Lethal Dose 50 , Longevity/drug effects , Longevity/genetics , Longevity/radiation effects , X Chromosome/drug effects , X Chromosome/genetics , X Chromosome/radiation effects , X-Rays/adverse effectsABSTRACT
Under the influence of ethyl methanesulfonate the series of both morphological and structural mutants with different types of brain changes has been obtained in Drosophila melanogaster Oregon R strain. In the future, this collection of mutants will be used in the investigations of genetic control of brain degeneration and possible ways of brain regeneration.
Subject(s)
Brain/cytology , Drosophila melanogaster/drug effects , Ethyl Methanesulfonate/pharmacology , Mutagens/pharmacology , Animals , Brain/drug effects , Drosophila melanogaster/cytology , Drosophila melanogaster/genetics , Female , Male , Mutation , X Chromosome/drug effectsABSTRACT
The genetic instability of Drosophila melanogaster genes induced by the mobile genetic elements is reviewed. The main attention is paid to genetic instability depended on types of crossing. Data on the possibility of genetic instability induction by the chemical and physical (X-rays, heat-shock) agents and their complex effect are cited. It was shown that a number of agents which cause mutagenic effect realize their action by involving of mobile genetic elements.
Subject(s)
Drosophila melanogaster/genetics , Genes, Insect/genetics , Genetic Variation/genetics , Interspersed Repetitive Sequences/genetics , Animals , Crossing Over, Genetic/drug effects , Crossing Over, Genetic/genetics , Crossing Over, Genetic/radiation effects , Drosophila melanogaster/drug effects , Drosophila melanogaster/radiation effects , Genes, Insect/drug effects , Genes, Insect/radiation effects , Genetic Variation/drug effects , Genetic Variation/radiation effects , Interspersed Repetitive Sequences/drug effects , Interspersed Repetitive Sequences/radiation effectsABSTRACT
The influence of mitomycin C on the genetic stability of Drosophila laboratory lines was investigated. This mutagen caused one-locus and multi-loci mutations with high frequencies (approximately 10(-2), 10(-3)) in the first and the second generations. It is locus-specific mutagenesis. In situ hybridization experiments indicated the existence of primary and new sites of location of mobile genetic elements mdg 1, mdg 2, mdg 4. This instability was caused by activation of transposition and recombination.
