ABSTRACT
The dynamics of redox potential, pH, CH4, H2, CO2, N2 formation under the community development under anaerobic conditions in presence of acceptors of electrons (potassium ferricyanide, molecular oxygen, sodium nitrate and sodium sulphate) has been investigated. It has been established that the optimal value at the redox potential under the development of methanogenic community was created at the expense of microorganisms vital activity. The decrease of methanogenesis under the electron acceptors introduction is connected with the increase of the culture medium redox potential.
Subject(s)
Euryarchaeota/metabolism , Anaerobiosis , Carbon Dioxide/chemistry , Culture Media , Euryarchaeota/growth & development , Ferricyanides , Hydrogen/chemistry , Hydrogen-Ion Concentration , Methane/metabolism , Nitrates , Nitrogen/chemistry , Oxidation-Reduction , Oxygen , SulfatesABSTRACT
Modified Hungate vessel made of native penicillinum bottles and chemical vessels has been created and experimentally studied. The vessels can be used for cultivation of facultative and obligate anaerobe microorganisms on liquid and solid nutrient media. Locking devices of the vessel are described.
Subject(s)
Bacteria, Anaerobic/growth & development , Bacteriological Techniques/instrumentation , Culture MediaABSTRACT
Active sulphate-reducing microorganism which belongs to the genus Pseudomonas has been distinguished and described. The culture is a facultative aerobe, optimum Eh is -170-180 mV. Pseudomonas sp. being cultivated under strictly anaerobic conditions sulphate-reduction proceeds more intensively than under aerobic conditions. This fact should be taken into account under treatment of industrial sewage.