[Enhancement of epidermal regeneration by recombinant vaccinia virus growth factor]. / Usilenie épidermal'noi regeneratsii s pomoshch'iu rekombinantnogo faktora rosta virusa ospovaktsiny.

Examining the specific activity has showed that recombinant vaccinia virus growth factor binds to appropriate receptors on the A-431 cell surface and prompts the healing acceleration of degree III burns in rats. This recombinant factor did not demonstrate pyrogenicity or toxicogenicity in tests on rabbits, guinea-pits, noninbred albino mice.

[Recombinant vaccinia virus expressing Japanese encephalitis virus protein E]. / Rekombinantnyi virus ospovaktsiny, ékspressiruiushchii belok E virusa iaponskogo zéntsefalita.

Recombinant vaccinia virus expressing protein E of Japanese encephalitis virus has been constructed. Polyclonal antibodies to JE virus reacted with recombinant protein E in immunoblotting. Immunochemical analysis of the recombinant protein E with monoclonal antibodies showed that both group specific and receptor domains of the protein were intact.

[Expression of a synthetic gene for human angiogenin in a recombinant vaccinia virus]. / Ekspressiia sinteticheskogo gena angiogenina cheloveka v sostave virusa ospovaktsiny.

The gene for angiogenin was cloned into vaccinia virus genome. The recombinant virus expressing angiogenin was obtained. The level of protein synthesis directed by the recombinant virus was analyzed by immunoblotting using monoclonal antibodies against human angiogenin.

[Immunochemical analysis of Escherichia coli expression products of I3 and A2 genes of vaccinia virus strain L-IVL genome]. / Immunokhimicheskii analiz produktov ékspressii v Escherichia coli genov I3 i A2 genoma virusa ospovaktsiny shtamma L-IVL.

Genes I3 and A2 of the vaccinia virus strain L-IVP DNA were cloned into bacterial expressing vectors. The monospecific antisera to the expression products of these genes in E. coli were obtained. By means of immunochemical cross-analysis two polypeptides of equal electrophoretic mobility were found in the virion preparations in the band of the major envelope protein p35. The major of them is the product of gene I3, and the minor is encoded by gene A2.

[Mapping the genes of the vaccinia virus, coding membrane proteins p34 and p40, by mRNA hybridization selection]. / Kartirovanie genov virusa ospovaktsiny, kodiruiushchikh membrannye belki p34 i p40, metodom gibridizatsionnoi selektsii mRNK.

The HindIII--J HindIII-F fragments of the vaccinia virus DNA strain Lister have been analysed by the technique of mRNA hybridization selection with the subsequent translation in cell-free protein synthesizing system from the rabbit reticulocytes. The viral mRNA hybridizable with the HindIII--J fragment was shown to direct the synthesis of 30 kDa polypeptide in the cell-free system. This polypeptide was demonstrated to react specifically with antiserum to plasma membrane protein p34. The viral mRNA hybridizable with the HindIII-F fragment was shown to direct the synthesis of 37 kDa polypeptide in the cell-free system. This polypeptide reacts specifically with antiserum to major membrane protein p40.

[Isolation and characteristics of vaccinia virus proteins associated with cell membranes of infected cells]. / Vydelenie i kharakteristika belkov virusa ospovaktsiny, assotsirovannykh s plazmaticheskimi membranami infitsirovannykh kletok.

The proteins of vaccinia virus associated with plasma membrane infected cells BHK-21, p60, p45, p42, p40,p35,p34,p28,p23 were isolated from plasma membranes using affinity chromatography, gel-electrophoresis and passive elution. An immunochemical characterization was carried out using specific antiserum to these proteins. Investigation of temporal regulation of proteins synthesis in infected cells showed that proteins p60, p45, p42, p40, p28 were late, and p35, p34, p23--early-late proteins. Immunochemical analysis of vaccinia virus mRNA cell-free translational products was carried out using specific antiserum. The polypeptide-precursors of viral proteins were identified.

[Molecular-biological study of vaccinia virus genome. III. Identification of the late gene product protein 36K from Hind-III-P-fragment of vaccinia virus strain L-IVP]. / Molekuliarno-biologicheskoe izuchenie genoma virusa ospovaktsiny. III. Identifikatsiia produkta pozdnego gena belka 36K iz HindIII-P-fragmenta genoma virusa ospovaktsiny shtamma L-IVP.

Vaccinia virus gene encoding 36K protein was cloned in pUR290 bacterial expressing vector and resulted in the synthesis of a chimeric protein in E. coli. The chimeric protein consists of beta-galactosidase and virus protein in C-termini. It has virus antigen specificity. By monospecific antibody 36K protein of vaccinia virus was determined to be non-virion. It is localized in the cytoplasm of infected cells.