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1.
Hum Immunol ; 79(5): 373-379, 2018 May.
Article in English | MEDLINE | ID: mdl-29458071

ABSTRACT

Transplantation is currently the therapy of choice for endstage organ failure even though it requires long-term immunosuppresive therapy, with its numerous side effects, for acceptance of the transplanted organ. In rare cases however, patients develop operational tolerance, that is, graft survival without immunosuppression. Studies conducted on these patients reveal genetic, phenotypic, and functional signatures. They provide a better understanding of the immunological mechanisms involved in operational tolerance and define biomarkers that could be used to adapt immunosuppressive treatment to the individual, safely reduce immunosuppression doses, and ideally and safely guide immunosuppression withdrawal. This review summarizes studies that suggest a role for B cells as biomarkers of operational tolerance and discusses the use of B cells as a predictive tool for immunologic risk.


Subject(s)
B-Lymphocytes/immunology , Transplantation Tolerance/immunology , B-Lymphocyte Subsets/cytology , B-Lymphocyte Subsets/immunology , B-Lymphocytes/cytology , Biomarkers/metabolism , Cell Differentiation/immunology , Graft Rejection/genetics , Graft Rejection/immunology , Graft Survival/genetics , Graft Survival/immunology , Humans , Models, Immunological , Receptors, Antigen, B-Cell/immunology , Receptors, Antigen, B-Cell/metabolism , Transplantation Tolerance/genetics
2.
Am J Transplant ; 17(6): 1490-1501, 2017 Jun.
Article in English | MEDLINE | ID: mdl-27888555

ABSTRACT

Renal operationally tolerant patients (TOL) display a defect in B cell differentiation, with a deficiency in plasma cells. Recently described, T follicular helper (Tfh) cells play a critical role in B cell differentiation. We analyzed blood Tfh subsets in TOL and transplanted patients with stable graft function under immunosuppression (STA). We observed a reduced proportion of blood activated and highly functional Tfh subsets in TOL, without affecting Tfh absolute numbers. Functionally, Tfh cells from TOL displayed a modified gene expression profile, failed to produce interleukin-21, and were unable to induce IgG production by naive B cells. This Tfh defect is linked to a low incidence of postgraft de novo donor-specific antibody (dnDSA) immunization, suggesting that the lack of Tfh cells in TOL may induce a protolerogenic environment with reduced risk of developing dnDSA. Finally, we showed that elevated Tfh in STA precedes the occurrence of dnDSA during an alloresponse. These data provide new insights into the mechanisms of antibody response in operational tolerance. Disrupted homeostasis and impaired Tfh function in TOL could lead to a reduced risk of developing dnDSA and suggest a predictive role of blood Tfh cells on the occurrence of dnDSA in transplant recipients.


Subject(s)
B-Lymphocytes/immunology , Graft Rejection/immunology , Immune Tolerance/immunology , Isoantibodies/immunology , Kidney Transplantation , Plasma Cells/immunology , T-Lymphocytes, Helper-Inducer/immunology , Adult , Aged , Antibody Formation/immunology , Cell Differentiation , Female , Follow-Up Studies , Glomerular Filtration Rate , Graft Survival , Humans , Interleukins/metabolism , Kidney Failure, Chronic/surgery , Kidney Function Tests , Lymphocyte Activation , Male , Middle Aged , Prognosis , Risk Factors , T-Lymphocytes, Helper-Inducer/pathology , Transplant Recipients
3.
Am J Transplant ; 16(11): 3255-3261, 2016 11.
Article in English | MEDLINE | ID: mdl-27367750

ABSTRACT

Long-term renal transplant outcome is limited by side effects of immunosuppressive drugs, particularly calcineurin inhibitor (CNI). We assumed that some patients selected for a "low immunological risk of rejection" could be eligible and benefit from a CNI weaning strategy. We designed a prospective, randomized, multicenter, double-blind placebo-controlled clinical study (Eudract: 2010-019574-33) to analyze the benefit-risk ratio of tacrolimus weaning on highly selected patients (≥4 years of transplantation, normal histology, stable graft function, no anti-HLA immunization). The primary endpoint was improvement of renal function. Fifty-two patients were scheduled in each treatment arm, placebo compared to the CNI maintenance arm. Only 10 patients were eligible and randomized. Five patients were assigned to the placebo arm and five were assigned to the tacrolimus maintenance arm. In the tacrolimus maintenance arm, all patients maintained stable graft function and no immunological events occurred. Contrastingly, in the placebo arm, all five patients had to reintroduce a full dose of tacrolimus since three of them presented an acute rejection episode (one humoral, one mixed, and one borderline) and two displayed anti-HLA antibodies without histological lesion (one donor-specific antibodies [DSA] and one non-DSA). Clearly, tacrolimus withdrawal must be avoided even in long-term highly selective stable kidney recipients.


Subject(s)
Graft Rejection/drug therapy , Kidney Failure, Chronic/surgery , Kidney Transplantation/adverse effects , Tacrolimus/administration & dosage , Weaning , Adolescent , Adult , Aged , Aged, 80 and over , Calcineurin Inhibitors/administration & dosage , Double-Blind Method , Female , Follow-Up Studies , Glomerular Filtration Rate , Graft Rejection/etiology , Graft Survival/drug effects , Humans , Immunosuppressive Agents/therapeutic use , Kidney Function Tests , Male , Middle Aged , Postoperative Complications/drug therapy , Prospective Studies , Transplant Recipients , Treatment Failure , Young Adult
4.
Am J Transplant ; 14(1): 144-55, 2014 Jan.
Article in English | MEDLINE | ID: mdl-24354874

ABSTRACT

Operationally tolerant patients (TOL) display a higher number of blood B cells and transcriptional B cell signature. As they rarely develop an allo-immune response, they could display an abnormal B cell differentiation. We used an in vitro culture system to explore T-dependent differentiation of B cells into plasma cells. B cell phenotype, apoptosis, proliferation, cytokine, immunoglobulin production and markers of differentiation were followed in blood of these patients. Tolerant recipients show a higher frequency of CD20(+) CD24(hi) CD38(hi) transitional and CD20(+) CD38(lo) CD24(lo) naïve B cells compared to patients with stable graft function, correlating with a decreased frequency of CD20(-) CD38(+) CD138(+) differentiated plasma cells, suggestive of abnormal B cell differentiation. B cells from TOL proliferate normally but produce more IL-10. In addition, B cells from tolerant recipients exhibit a defective expression of factors of the end step of differentiation into plasma cells and show a higher propensity for cell death apoptosis compared to patients with stable graft function. This in vitro profile is consistent with down-regulation of B cell differentiation genes and anti-apoptotic B cell genes in these patients in vivo. These data suggest that a balance between B cells producing IL-10 and a deficiency in plasma cells may encourage an environment favorable to the tolerance maintenance.


Subject(s)
Apoptosis/immunology , B-Lymphocytes/immunology , Cell Differentiation/immunology , Immune Tolerance/immunology , Kidney Transplantation , Plasma Cells/cytology , Adult , Antigens, CD/immunology , Cells, Cultured , Down-Regulation , Female , Humans , Interleukin-10/biosynthesis , Lymphocyte Activation , Lymphocyte Count , Male , Middle Aged
5.
Ann Gastroenterol Hepatol (Paris) ; 23(5): 257-60, 1987 Oct.
Article in French | MEDLINE | ID: mdl-3314660

ABSTRACT

In this paper, the authors discuss an occurrence of hepatic sarcoidosis in a female patient from the French Antilles which was unique in how it was detected (during cholecystectomy), its presence in two organs (bronchus and liver) as evidenced histologically, as well as marked immunological disorders and disturbances of liver function tests. With corticosteroid therapy, the liver function tests were normalized, but histological lesions persisted.


Subject(s)
Liver Diseases/pathology , Sarcoidosis/pathology , Cholecystectomy , Female , Humans , Liver Diseases/complications , Liver Diseases/drug therapy , Middle Aged , Prednisolone/therapeutic use , Sarcoidosis/complications , Sarcoidosis/drug therapy , Urinary Bladder Calculi/complications , Urinary Bladder Calculi/surgery
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