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Two tRNA gene clusters associated with rRNA operons rrnD and rrnE in Bacillus subtilis.

Rudner, R; Chevrestt, A; Buchholz, S R; Studamire, B; White, A M; Jarvis, E D.

J Bacteriol ; 175(2): 503-9, 1993 Jan.

Article in English | MEDLINE | ID: mdl-8419296

ABSTRACT

Sequence analysis of cloned rescued DNA fragments from a Bacillus subtilis strain with an inserted recombinant plasmid in ribosomal operon rrnE revealed the presence of two tRNA genes for Met and Asp at the 3' end of the operon. Probing chromosomal DNA from a strain carrying a plasmid inserted in rrnD with a fragment containing the genetically unassigned cluster of 16 tRNA genes revealed that the cluster is located immediately following the rrnD operon. Our findings show that all 10 rrn operons in B. subtilis are associated with tRNA gene clusters.

Subject(s)

Bacillus subtilis/genetics , Genes, Bacterial , Multigene Family , Operon , RNA, Ribosomal/genetics , RNA, Transfer/genetics , Amino Acid Sequence , Base Sequence , Blotting, Southern , Chromosome Mapping , Chromosomes, Bacterial , DNA, Bacterial/genetics , DNA, Bacterial/isolation & purification , DNA, Ribosomal/genetics , Molecular Sequence Data , Oligodeoxyribonucleotides , Polymerase Chain Reaction/methods , Restriction Mapping

ABSTRACT

Subject(s)

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