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1.
Front Neuroanat ; 9: 151, 2015.
Article in English | MEDLINE | ID: mdl-26640429

ABSTRACT

The high anatomical contrast achieved with the newly emerging MRI tractographic technique of super-resolution track density imaging (TDI) encouraged us to search for a new fiber tract in the septum pellucidum. Although this septum pellucidum tract (SPT) has been observed previously, its connections were unclear due to ambiguity and limited resolution of conventional MRI images. It is now possible to identify detailed parts of SPT with the increased resolution of TDI, which involves diffusion MRI imaging, whole-brain tractography, and voxel subdivision using the track-count information. Four healthy male subjects were included in the study. The experiment was performed with 7.0T MRI, following the guidelines of the institute's institutional review board. Data were processed with the super-resolution TDI technique to generate a tractographic map with 0.18 mm isotropic resolution. The SPT was identified in all subjects. Based on additional seed tracking method with inter-axis correlation search, we have succeeded in identifying a new frontal lobe pathway in the SPT. We hypothesize that the tract is connected as a superior dorsal branch of the fornix that leads to the prefrontal cortex.

2.
J Pathol Transl Med ; 49(6): 497-510, 2015 Nov.
Article in English | MEDLINE | ID: mdl-26471340

ABSTRACT

BACKGROUND: Prenatal tongue development may affect oral-craniofacial structures, but this muscular organ has rarely been investigated. METHODS: In order to document the physiology of prenatal tongue growth, we histologically examined the facial and cranial base structures of 56 embryos and 106 fetuses. RESULTS: In Streeter's stages 13-14 (fertilization age [FA], 28 to 32 days), the tongue protruded into the stomodeal cavity from the retrohyoid space to the cartilaginous mesenchyme of the primitive cranial base, and in Streeter's stage 15 (FA, 33 to 36 days), the tongue rapidly swelled and compressed the cranial base to initiate spheno-occipital synchondrosis and continued to swell laterally to occupy most of the stomodeal cavity in Streeter's stage 16-17 (FA, 37 to 43 days). In Streeter's stage 18-20 (FA, 44 to 51 days), the tongue was vertically positioned and filled the posterior nasopharyngeal space. As the growth of the mandible and maxilla advanced, the tongue was pulled down and protruded anteriorly to form the linguomandibular complex. Angulation between the anterior cranial base (ACB) and the posterior cranial base (PCB) was formed by the emerging tongue at FA 4 weeks and became constant at approximately 124°-126° from FA 6 weeks until birth, which was consistent with angulations measured on adult cephalograms. CONCLUSIONS: The early clockwise growth of the ACB to the maxillary plane became harmonious with the counter-clockwise growth of the PCB to the tongue axis during the early prenatal period. These observations suggest that human embryonic tongue growth affects ACB and PCB angulation, stimulates maxillary growth, and induces mandibular movement to achieve the essential functions of oral and maxillofacial structures.

3.
World Neurosurg ; 83(1): 54-61.e32, 2015 Jan.
Article in English | MEDLINE | ID: mdl-23973452

ABSTRACT

BACKGROUND: Images obtained through ultra-high-field 7.0-tesla magnetic resonance imaging with track-density imaging provide clear, high-resolution tractograms that have been hitherto unavailable, especially in deep brain areas such as the limbic and thalamic regions. This study is a largely pictorial description of the deep fiber tracts in the brain using track-density images obtained with 7.0-T diffusion-weighted imaging. METHODS: To identify the fiber tracts, we selected 3 sets of tractograms and performed interaxis correlation between them. These tractograms offered an opportunity to extract new information in areas that have previously been difficult to examine using either in vivo or in vitro human brain tractography. RESULTS: With this new technique, we identified 4 fiber tracts that have not previously been directly visualized in vivo: septum pellucidum tract, anterior thalamic radiation, superolateral medial forebrain bundle, and inferomedial forebrain bundle. CONCLUSIONS: We present the high-resolution images as a tool for researchers and clinicians working with neurodegenerative and psychiatric diseases, such as Parkinson disease, Alzheimer disease, and depression, in which the accurate positioning of deep brain stimulation is essential for precise targeting of nuclei and fiber tracts.


Subject(s)
Anterior Thalamic Nuclei/anatomy & histology , Diffusion Tensor Imaging/methods , Limbic System/anatomy & histology , Medial Forebrain Bundle/anatomy & histology , Nerve Fibers/ultrastructure , Septum Pellucidum/anatomy & histology , Thalamus/anatomy & histology , Adult , Humans , Image Processing, Computer-Assisted , Male
4.
J Korean Med Sci ; 29(10): 1416-24, 2014 Oct.
Article in English | MEDLINE | ID: mdl-25368497

ABSTRACT

The two basic scripts of the Korean writing system, Hanja (the logography of the traditional Korean character) and Hangul (the more newer Korean alphabet), have been used together since the 14th century. While Hanja character has its own morphemic base, Hangul being purely phonemic without morphemic base. These two, therefore, have substantially different outcomes as a language as well as different neural responses. Based on these linguistic differences between Hanja and Hangul, we have launched two studies; first was to find differences in cortical activation when it is stimulated by Hanja and Hangul reading to support the much discussed dual-route hypothesis of logographic and phonological routes in the brain by fMRI (Experiment 1). The second objective was to evaluate how Hanja and Hangul affect comprehension, therefore, recognition memory, specifically the effects of semantic transparency and morphemic clarity on memory consolidation and then related cortical activations, using functional magnetic resonance imaging (fMRI) (Experiment 2). The first fMRI experiment indicated relatively large areas of the brain are activated by Hanja reading compared to Hangul reading. The second experiment, the recognition memory study, revealed two findings, that is there is only a small difference in recognition memory for semantic transparency, while for the morphemic clarity was much larger between Hanja and Hangul. That is the morphemic clarity has significantly more effect than semantic transparency on recognition memory when studies by fMRI in correlation with behavioral study.


Subject(s)
Brain Mapping/methods , Brain Waves/physiology , Brain/physiology , Adult , Female , Humans , Magnetic Resonance Imaging , Male , Neurolinguistic Programming , Recognition, Psychology/physiology , Writing
6.
Radiology ; 272(2): 541-8, 2014 Aug.
Article in English | MEDLINE | ID: mdl-24654972

ABSTRACT

PURPOSE: To measure the activity of individual raphe nuclei with fluorine 18 fluorodeoxyglucose (FDG) and carbon 11 ((11)C) 3-amino-4-(2-dimethylaminomethylphenylthio) benzonitrile (DASB) imaging using a brain positron emission tomography(PET)/magnetic resonance (MR) imaging fusion system. MATERIALS AND METHODS: The study was approved by the Institutional Review Board of Gil Medical Center, and all volunteers provided written informed consent. FDG PET, (11)C-DASB PET, and T2*-weighted MR images from seven healthy volunteers were acquired by using a PET/MR imaging fusion system. The standard uptake value ratio (SUVR) of FDG (FDG-SUVR) and nondisplaceable binding potential (BPnd) of (11)C-DASB (DASB-BPnd) were determined for each raphe nucleus. A Pearson correlation analysis was performed to show the correlation between FDG-SUVR and DASB-BPnd for the raphe nuclei. RESULTS: Each raphe nucleus could be distinguished in both FDG (identifiability ratio, 0.86; κ = 0.77) and (11)C-DASB (identifiability ratio, 0.89; κ = 0.72) images. The mean values of DASB-BPnd for each raphe nucleus from dorsal to caudal direction were 6.08 (raphe nucleus 1), 5.93 (raphe nucleus 2), 3.86 (raphe nucleus 3), 3.18 (raphe nucleus 4), and 2.74 (raphe nucleus 5); the mean FDG-SUVR values were 1.00 (raphe nucleus 1), 1.00 (raphe nucleus 2), 0.87 (raphe nucleus 3), 0.94 (raphe nucleus 4), and 0.90 (raphe nucleus 5). FDG-SUVR and DASB-BPnd for the raphe nuclei were significantly correlated (r = 0.506, P = .002). CONCLUSION: Serotonergic activity, both glucose metabolism and transporter binding potential of raphe nuclei, were measured with a brain-dedicated PET/MR imaging system and showed a significant correlation.


Subject(s)
Magnetic Resonance Imaging/methods , Multimodal Imaging , Positron-Emission Tomography/methods , Raphe Nuclei/anatomy & histology , Adult , Benzylamines/administration & dosage , Carbon Radioisotopes/administration & dosage , Female , Fluorodeoxyglucose F18/administration & dosage , Humans , Male , Radiopharmaceuticals/administration & dosage
7.
Korean J Radiol ; 14(4): 653-61, 2013.
Article in English | MEDLINE | ID: mdl-23901324

ABSTRACT

OBJECTIVE: The anterior commissure (AC) and posterior commissure (PC) are the two distinct anatomic structures in the brain which are difficult to observe in detail with conventional MRI, such as a 1.5T MRI system. However, recent advances in ultra-high resolution MRI have enabled us to examine the AC and PC directly. The objective of the present study is to standardize the shape and size of the AC and PC using a 7.0T MRI and to propose a new brain reference line. MATERIALS AND METHODS: Thirty-four, 21 males and 13 females, healthy volunteers were enrolled in this study. After determining the center of each AC and PC, we defined the connection of these centers as the central intercommissural line (CIL). We compared the known extra- and intra-cerebral reference lines with the CIL to determine the difference in the angles. Additionally, we obtained horizontal line from flat ground line of look front human. RESULTS: The difference in angle of the CIL and the tangential intercommissural line (TIL) from the horizontal line was 8.7 ± 5.1 (11 ± 4.8) and 17.4 ± 5.2 (19.8 ± 4.8) degrees in males and females, respectively. The difference in angle between the CIL and canthomeatal line was 10.1 in both male and female, and there was no difference between both sexes. Likewise, there was no significant difference in angle between the CIL and TIL between both sexes (8.3 +/- 1.1 in male and 8.8 +/- 0.7 in female). CONCLUSION: In this study, we have used 7.0T MRI to define the AC and PC quantitatively and in a more robust manner. We have showed that the CIL is a reproducible reference line and serves as a standard for the axial images of the human brain.


Subject(s)
Brain/anatomy & histology , Magnetic Resonance Imaging/methods , Adult , Brain Mapping/methods , Female , Healthy Volunteers , Humans , Male , Reproducibility of Results , Young Adult
8.
Hum Brain Mapp ; 34(10): 2538-48, 2013 Oct.
Article in English | MEDLINE | ID: mdl-23151892

ABSTRACT

The thalamus is one of the most important brain structures, with strong connections between subcortical and cortical areas of the brain. Most of the incoming information to the cortex passes through the thalamus. Accurate identification of substructures of the thalamus is therefore of great importance for the understanding of human brain connectivity. Direct visualization of thalamic substructures, however, is not easily achieved with currently available magnetic resonance imaging (MRI), including ultra-high field MRI such as 7.0T, mainly due to the limited contrast between the relevant structures. Recently, improvements in ultra-high field 7.0T MRI have opened the possibility of observing thalamic substructures by well-adjusted high-resolution T1 -weighted imaging. Moreover, the recently developed super-resolution track-density imaging (TDI) technique, based on results from whole-brain fiber-tracking, produces images with sub-millimeter resolution. These two methods enable us to show markedly improved anatomical detail of the substructures of the thalamus, including their detailed locations and directionality. In this study, we demonstrate the role of TDI for the visualization of the substructures of the thalamic nuclei, and relate these images to T1-weighted imaging at 7.0T MRI.


Subject(s)
Diffusion Tensor Imaging/methods , Echo-Planar Imaging/methods , Imaging, Three-Dimensional/methods , Thalamic Nuclei/ultrastructure , Adult , Algorithms , Female , Humans , Male
9.
Ann Neurol ; 71(2): 267-77, 2012 Feb.
Article in English | MEDLINE | ID: mdl-22367998

ABSTRACT

OBJECTIVE: To investigate anatomical changes in the substantia nigra (SN) of Parkinson disease (PD) patients with age-matched controls by using ultra-high field magnetic resonance imaging (MRI). METHODS: We performed 7T MRI in 10 PD and 10 age-matched control subjects. Magnetic resonance images of the SN were obtained from a 3-dimensional (3D) T(2)*-weighted gradient echo sequence. Region of interest-based 3D shape analysis was performed to quantitatively compare images from the 2 groups. RESULTS: The boundary between the SN and crus cerebri was not smooth in PD subjects. Undulation in the lateral surface of the SN appeared more intense in the side contralateral to that with the more severe symptoms, and more prominent at the rostral level of the SN than at the intermediate or caudal levels. In addition to the lateral surface, there was a striking difference in the dorsomedial aspects of the SN between PD and control subjects. In control subjects, a brighter signal region was observed along the dorsomedial surface of the lateral portion of SN, whereas in PD subjects, this region was observed as a dark region containing a hypointense signal in T(2)*-weighted images. The measurement of SN volumes, normalized to the intracranial volumes, showed higher values in PD subjects than in control subjects. INTERPRETATION: This study demonstrates that 3D 7T MRI can definitively visualize anatomical alterations occurring in the SN of PD subjects. Further pathological studies are required to elucidate the nature of these anatomical alterations.


Subject(s)
Magnetic Resonance Imaging/methods , Parkinson Disease/pathology , Substantia Nigra/pathology , Aged , Female , Humans , Magnetic Resonance Imaging/instrumentation , Male , Middle Aged , Parkinson Disease/diagnosis
10.
Neuroimage ; 59(2): 1094-7, 2012 Jan 16.
Article in English | MEDLINE | ID: mdl-21963920

ABSTRACT

UNLABELLED: The brainstem contains various important monoaminergic neuronal centers, including the raphe nuclei which contain serotonergic neurons. The raphe nuclei, however, are not easily identifiable and located by conventional neuroimaging. METHODS: Fluorodeoxyglucose positron emission tomography (PET) and magnetic resonance imaging (MRI) were performed in seven healthy subjects using a new PET-MRI, which consists of a high-resolution research tomograph (HRRT) PET and 7.0 T-MRI. Glucose metabolism of raphe nuclei was semiquantitatively measured and identified along the midline brainstem region in vivo. RESULTS: Midline nuclei clustered in four groups appeared to be the raphe nuclei and could be clearly visualized; specifically, we identified the groups as the dorsal raphe, raphe reticularis centralis superior, raphe pontis, and raphe magnus group. CONCLUSION: FDG imaging of the midline raphe nuclei in vivo could potentially be an important tool for investigating brain diseases as well as conducting functional brain studies in the context of sleep disorders, depression, and neurodegenerative disease.


Subject(s)
Fluorodeoxyglucose F18/pharmacokinetics , Glucose/metabolism , Magnetic Resonance Imaging/methods , Positron-Emission Tomography/methods , Raphe Nuclei/metabolism , Subtraction Technique , Female , Humans , Male , Metabolic Clearance Rate , Radiopharmaceuticals/pharmacokinetics , Raphe Nuclei/diagnostic imaging , Raphe Nuclei/pathology , Tissue Distribution , Young Adult
11.
Mov Disord ; 26(4): 713-8, 2011 Mar.
Article in English | MEDLINE | ID: mdl-21506148

ABSTRACT

Parkinson's disease (PD) is a neurodegenerative disorder resulting from progressive loss of dopaminergic neurons in the substantia nigra (SN) pars compacta. Therefore, imaging of the SN has been regarded to hold greatest potential for use in the diagnosis of PD. At the 7.0T magnetic resonance imaging (MRI), it is now possible to delineate clearly the shapes and boundaries of the SN. We scanned eight early and two advanced PD patients, along with nine age-matched control subjects, using a 7.0T MRI in an attempt to directly visualize the SN and quantify the differences in shape and boundaries of SN between PD subjects in comparison with the normal control subjects. In the normal controls, the boundaries between the SN and crus cerebri appear smooth, and clean "arch" shapes that stretch ventrally from posterior to anterior. In contrast, these smooth and clean arch-like boundaries were lost in PD subjects. The measured correlation analyses show that, in PD patients, there is age-dependent correlation and substantially stronger UPDRS motor score-dependent correlation. These results suggest that, by using 7.0T MRI, it appears possible to use these visible and distinctive changes in morphology as a diagnostic marker of PD.


Subject(s)
Magnetic Resonance Imaging , Parkinson Disease/pathology , Substantia Nigra/pathology , Adult , Aged , Brain Mapping , Case-Control Studies , Female , Functional Laterality , Humans , Image Processing, Computer-Assisted/methods , Male , Middle Aged
12.
J Nucl Med ; 52(3): 401-4, 2011 Mar.
Article in English | MEDLINE | ID: mdl-21321261

ABSTRACT

UNLABELLED: The anatomy of the thalamus and its connectivity with surrounding areas are known. Localized metabolic activities at the thalamic substructural level have not been measured in vivo in human brains because of limited resolution and contrast. METHODS: The energy metabolism and fine anatomic structures of the thalamus were measured simultaneously in 5 healthy subjects using a PET/MRI fusion imaging system. Measured metabolism in individual thalamic nuclei was quantified by corresponding PET/MRI images. RESULTS: Substructures of the thalamus were clearly distinguished in 7.0-T MRI images, and the corresponding metabolic activities measured by PET were integrated by the PET/MRI system. The medial dorsal thalamic nucleus consistently showed the highest glucose uptake among the thalamic nuclei. CONCLUSION: These results demonstrate that substructure-specific metabolic activities in the thalamus can be measured with a PET/MRI system consisting of an ultra-high-resolution PET component and an ultra-high-field MRI component.


Subject(s)
Fluorodeoxyglucose F18/pharmacokinetics , Glucose/metabolism , Magnetic Resonance Imaging/methods , Positron-Emission Tomography/methods , Thalamic Nuclei/diagnostic imaging , Thalamic Nuclei/metabolism , Adult , Female , Humans , Male , Metabolic Clearance Rate , Radiopharmaceuticals/pharmacokinetics , Subtraction Technique , Tissue Distribution
13.
J Korean Med Sci ; 25(12): 1710-5, 2010 Dec.
Article in English | MEDLINE | ID: mdl-21165283

ABSTRACT

Authors had prepared the high-quality sectioned images of a cadaver head. For the delineation of each cerebral gyrus, three-dimensional model of the same brain was required. The purpose of this study was to develop the segmentation protocol of cerebral gyri by referring to the three-dimensional model on the personal computer. From the 114 sectioned images (intervals, 1 mm), a cerebral hemisphere was outlined. On MRIcro software, sectioned images including only the cerebral hemisphere were volume reconstructed. The volume model was rotated to capture the lateral, medial, superior, and inferior views of the cerebral hemisphere. On these four views, areas of 33 cerebral gyri were painted with colors. Derived from the painted views, the cerebral gyri in sectioned images were identified and outlined on the Photoshop to prepare segmented images. The segmented images were used for production of volume and surface models of the selected gyri. The segmentation method developed in this research is expected to be applied to other types of images, such as MRIs. Our results of the sectioned and segmented images of the cadaver brain, acquired in the present study, are hopefully utilized for medical learning tools of neuroanatomy.


Subject(s)
Cerebrum/anatomy & histology , Models, Anatomic , Aged , Humans , Imaging, Three-Dimensional , Male , Software
14.
J Nucl Med ; 51(10): 1545-8, 2010 Oct.
Article in English | MEDLINE | ID: mdl-20847161

ABSTRACT

UNLABELLED: The hippocampus is one of the best-known neural structures in the brain and has been of interest in observing the substructures and their metabolic functions. However, it has been difficult to distinguish its substructures and functions in vivo because of its small size. METHODS: (18)F-FDG PET and high-resolution MRI of the hippocampus were performed on 5 healthy subjects using a PET/MRI system. The metabolism of each hippocampal substructure was measured in vivo on the basis of the MR images. RESULTS: The dentate gyrus and cornu ammonis 4 showed the highest glucose uptake in the healthy subjects. CONCLUSION: Measuring glucose metabolism in the substructures of the hippocampus could provide a new tool for the future investigation of related brain diseases or functional studies, such as Alzheimer disease or memory and learning studies.


Subject(s)
Fluorodeoxyglucose F18/pharmacokinetics , Glucose/metabolism , Hippocampus/anatomy & histology , Hippocampus/metabolism , Magnetic Resonance Imaging/methods , Positron-Emission Tomography/methods , Adult , Female , Humans , Male , Radiopharmaceuticals/pharmacokinetics , Tissue Distribution
15.
J Neurol Sci ; 295(1-2): 23-6, 2010 Aug 15.
Article in English | MEDLINE | ID: mdl-20547401

ABSTRACT

Despite a few case reports over the last 60 years, little progress has been made in defining the phenotype, genotype and pathophysiological mechanisms involved in pigmentary orthochromatic leukodystrophy (POLD). Furthermore, there is currently no data available regarding MRI in patients in the relatively early stages of POLD. Here, we present a 37 year old male patient with brain biopsy-proven POLD who had brain MRIs three times during the first year of his clinical course and proton MR spectroscopy (MRS) throughout his diagnostic evaluation. This patient with POLD was clinically characterized by seizures, rapidly progressive frontally predominant dementia and gait disturbance. The brain MRIs taken serially over the first year revealed progressive development of frontal-predominant white matter changes in the periventricular areas during the earlier periods, which later spread into the deep white matter. His MRS was helpful in the diagnostic approach because the results enabled demyelinating changes to be distinguished from other disease processes such as ischemia, gliosis or tumors. The MRS findings also reflected the disease dynamics because metabolic derangement was observed, even in the white matter that appeared normal. The findings presented here provide insight into the dynamics of POLD.


Subject(s)
Brain Diseases/diagnosis , Brain/pathology , Demyelinating Diseases/diagnosis , Magnetic Resonance Spectroscopy , Adult , Antigens, CD/metabolism , Aspartic Acid/analogs & derivatives , Aspartic Acid/metabolism , Brain/metabolism , Brain Diseases/complications , Brain Mapping , Creatine/metabolism , Demyelinating Diseases/complications , Humans , Magnetic Resonance Imaging , Male
16.
J Neurosurg ; 113(3): 639-47, 2010 Sep.
Article in English | MEDLINE | ID: mdl-20380532

ABSTRACT

OBJECT: A challenge associated with deep brain stimulation (DBS) in treating advanced Parkinson disease (PD) is the direct visualization of brain nuclei, which often involves indirect approximations of stereotactic targets. In the present study, the authors compared T2*-weighted images obtained using 7-T MR imaging with those obtained using 1.5- and 3-T MR imaging to ascertain whether 7-T imaging enables better visualization of targets for DBS in PD. METHODS: The authors compared 1.5-, 3-, and 7-T MR images obtained in 11 healthy volunteers and 1 patient with PD. RESULTS: With 7-T imaging, distinct images of the brain were obtained, including the subthalamic nucleus (STN) and internal globus pallidus (GPi). Compared with the 1.5- and 3-T MR images of the STN and GPi, the 7-T MR images showed marked improvements in spatial resolution, tissue contrast, and signal-to-noise ratio. CONCLUSIONS: Data in this study reveal the superiority of 7-T MR imaging for visualizing structures targeted for DBS in the management of PD. This finding suggests that by enabling the direct visualization of neural structures of interest, 7-T MR imaging could be a valuable aid in neurosurgical procedures.


Subject(s)
Deep Brain Stimulation/methods , Magnetic Resonance Imaging , Parkinson Disease/pathology , Parkinson Disease/therapy , Therapy, Computer-Assisted/methods , Adult , Brain/pathology , Feasibility Studies , Globus Pallidus/pathology , Humans , Magnetic Resonance Imaging/instrumentation , Magnetic Resonance Imaging/methods , Middle Aged , Phantoms, Imaging , Subthalamic Nucleus/pathology , Therapy, Computer-Assisted/instrumentation , Young Adult
17.
Mod Pathol ; 23(7): 1000-11, 2010 Jul.
Article in English | MEDLINE | ID: mdl-20348884

ABSTRACT

Acute chorioamnionitis is a well-established lesion of the placenta in cases with intra-amniotic infection. In contrast, the clinicopathological significance of chronic chorioamnionitis is unclear. This study was conducted to determine the frequency and severity of chronic chorioamnionitis in normal pregnancy and in various pregnancy complications. Placentas from the following patient groups were studied: (1) term not in labor (n=100), (2) term in labor (n=100), (3) preterm labor (n=100), (4) preterm prelabor rupture of membranes (n=100), (5) preeclampsia at term (n=100), (6) preterm preeclampsia (n=100), and (7) small-for-gestational-age at term (n=100). Amniotic fluid CXCL10 concentration was measured in 64 patients. CXCL9, CXCL10, and CXCL11 mRNA expressions in the chorioamniotic membranes were assessed using real-time quantitative reverse transcription-PCR. The frequency of chronic chorioamnionitis in the preterm labor group and the preterm prelabor rupture of membranes group was 34 and 39%, respectively, which was higher than that of normal-term placentas (term not in labor, 19%; term in labor, 8%; P<0.05 each). The frequency of chronic chorioamnionitis in the preeclampsia at term group, preterm preeclampsia group, and small-for-gestational-age group was 23, 16, and 13%, respectively. Concomitant villitis of unknown etiology was found in 38 and 36% of preterm labor cases and preterm prelabor rupture of membranes cases with chronic chorioamnionitis, respectively. Interestingly, the median gestational age of preterm chronic chorioamnionitis cases was higher than that of acute chorioamnionitis cases (P<0.05). The median amniotic fluid CXCL10 concentration was higher in cases with chronic chorioamnionitis than in those without, in both the preterm labor group and preterm prelabor rupture of membranes group (P<0.05 and P<0.01, respectively). CXCL9, CXCL10, and CXCL11 mRNA expression in the chorioamniotic membranes was also higher in cases with chronic chorioamnionitis than in those without chronic chorioamnionitis (P<0.05). We propose that chronic chorioamnionitis defines a common placental pathological lesion among the preterm labor and preterm prelabor rupture of membranes groups, especially in cases of late preterm birth. Its association with villitis of unknown etiology and the chemokine profile in amniotic fluid suggests an immunological origin, akin to transplantation rejection and graft-versus-host disease in the chorioamniotic membranes.


Subject(s)
Chorioamnionitis/epidemiology , Chorioamnionitis/pathology , Pregnancy Complications/epidemiology , Pregnancy Complications/pathology , Adult , Amniotic Fluid/chemistry , Amniotic Fluid/metabolism , Chemokine CXCL10/biosynthesis , Chemokine CXCL11/biosynthesis , Chemokine CXCL9/biosynthesis , Chorioallantoic Membrane/chemistry , Chorioallantoic Membrane/metabolism , Chorioamnionitis/metabolism , Chronic Disease , Enzyme-Linked Immunosorbent Assay , Female , Fetal Membranes, Premature Rupture/epidemiology , Fetal Membranes, Premature Rupture/metabolism , Fetal Membranes, Premature Rupture/pathology , Humans , Immunohistochemistry , Obstetric Labor, Premature/epidemiology , Obstetric Labor, Premature/metabolism , Obstetric Labor, Premature/pathology , Pre-Eclampsia/epidemiology , Pre-Eclampsia/metabolism , Pre-Eclampsia/pathology , Pregnancy , Pregnancy Complications/metabolism , Premature Birth/epidemiology , Premature Birth/metabolism , Premature Birth/pathology , RNA, Messenger/analysis , Reverse Transcriptase Polymerase Chain Reaction , Young Adult
18.
J Korean Med Sci ; 25(1): 135-41, 2010 Jan.
Article in English | MEDLINE | ID: mdl-20052359

ABSTRACT

Sectional anatomy of human brain is useful to examine the diseased brain as well as normal brain. However, intracerebral reference points for the axial, sagittal, and coronal planes of brain have not been standardized in anatomical sections or radiological images. We made 2,343 serially-sectioned images of a cadaver head with 0.1 mm intervals, 0.1 mm pixel size, and 48 bit color and obtained axial, sagittal, and coronal images based on the proposed reference system. This reference system consists of one principal reference point and two ancillary reference points. The two ancillary reference points are the anterior commissure and the posterior commissure. And the principal reference point is the midpoint of two ancillary reference points. It resides in the center of whole brain. From the principal reference point, Cartesian coordinate of x, y, z could be made to be the standard axial, sagittal, and coronal planes.


Subject(s)
Brain/anatomy & histology , Aged , Anatomy, Cross-Sectional , Brain/diagnostic imaging , Brain Mapping , Cadaver , Humans , Image Processing, Computer-Assisted , Male , Tomography, X-Ray Computed
19.
Neuroimage ; 49(3): 2134-40, 2010 Feb 01.
Article in English | MEDLINE | ID: mdl-19909820

ABSTRACT

In-vivo volumetric measurements of hippocampus have proven to be highly informative for studying various neurological diseases such as Alzheimer's disease. The usefulness of volumetric imaging, however, has been limited due to the poor image resolutions obtained by currently available MRI images. In this study, a new result of volumetric image measurement of the hippocampus using 7.0 T MRI images of high contrast and resolution is described. To verify the usefulness of the proposed method, its reliability and sensitivity were examined and compared with existing imaging techniques such as 1.5 T or 3.0 T MRI imaging. The results of our study with 7.0 T MRI clearly demonstrated superior boundary detection for the hippocampal head, body, and tail compared with low field MRIs. In conclusion, robust and reproducible volumetric measurements as well as 3D images of clear contrast obtained with 7.0 T suggest the usefulness of high field MR imaging and its eventual use for the accurate diagnosis of hippocampal diseases and related research.


Subject(s)
Hippocampus/anatomy & histology , Magnetic Resonance Imaging/methods , Adult , Female , Humans , Image Interpretation, Computer-Assisted , Magnetic Resonance Imaging/instrumentation , Male , Reproducibility of Results , Sensitivity and Specificity
20.
Cleft Palate Craniofac J ; 45(2): 131-40, 2008 Mar.
Article in English | MEDLINE | ID: mdl-18333644

ABSTRACT

OBJECTIVE: To elucidate abnormal growth patterns of human fetal maxillae with cleft lip and palate (CLP). SUBJECT: A total of 71 fetal maxillae with CLP were obtained from aborted human fetuses. METHOD: Dimensions of the maxillary trapezoid (MT), formed by the maxillary primary growth centers (MxPGC), were taken from radiographic images. The CLP dimensions were compared with maxillary trapezoid dimensions of normal fetuses from a previous study (Lee et al., 1992). MAIN OUTCOME MEASURES: Cleft lip subjects without a cleft palate, unilateral cleft lip-alveolar cleft or cleft palate (UCL+A/UCLP), and bilateral cleft lip-alveolar cleft or cleft palate (BCL+A/BCLP) displayed abnormal MT patterns. MT abnormalities were most marked in the BCL+A/BCLP cohort. RESULTS: The MT growth of prenatal CLP maxillae was severely arrested, resulting in abnormal MT shape on palatal radiograms. BCL+A/BCLP subjects had a more protruded nasal septum than subjects with other types of CLPs, while UCL+A/UCLP subjects showed severe deviation of the protruded nasal septum toward the noncleft side. Cleft lip-only subjects also exhibited abnormal MT growth. CONCLUSION: MT is primarily involved in CLPs, so that the MT shape could be utilized as a sensitive indicator for the analysis of maxillary malformation in different types of CLPs.


Subject(s)
Cleft Lip/embryology , Cleft Palate/embryology , Maxilla/abnormalities , Alveolar Process/abnormalities , Alveolar Process/embryology , Case-Control Studies , Cephalometry/methods , Cohort Studies , Gestational Age , Humans , Maxilla/embryology , Nasal Bone/embryology , Nasal Septum/embryology , Sphenoid Bone/embryology , Zygoma/embryology
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