[Expression and significance of secreted frizzled-related protein 1 and ß-catenin in gingival tissue of patients with chronic periodontitis].

OBJECTIVE: This study aimed to investigate the expression and correlation of secreted frizzled-related protein 1 (SFRP1) and ß-catenin in gingival tissues of patients with chronic periodontitis (CP). The role of the classical Wnt/ß-catenin signaling pathway in the development of periodontitis was also explored. METHODS: Twenty-eight patients with CP (CP group) were enrolled in this study. Among them, 16 cases were moderate CP, and 12 demonstrated severe CP. Twelve healthy cases comprised the controls (normal group). Gingival tissue was collected, and the probing depth, bleeding index, and clinical attachment loss were recorded. The expression levels of SFRP1 and ß-catenin were detected by immunohistochemistry, and staining intensity was evaluated by double scoring method. SPSS 19.0 was used for statistical analysis. RESULTS: The staining strength scores of SFRP1 and ß-catenin were 2.16±0.65 and 1.12±0.51 in the normal group, 3.57±0.45 and 2.36±0.49 in the CP group, 3.61±0.40 and 2.30±0.44 in the moderate CP group, and 3.52±0.52 and 2.45±0.55 in the severe CP group, respectively. The expression of SFRP1 and ß-catenin in the CP group was higher than that in the normal group (P<0.01). A significant difference was noted between the normal group and the moderate and severe CP groups (P<0.01) but none between the moderate and severe CP groups (P>0.05). A positive correlation was found between the expression of SFRP1 and ß-catenin (r=0.657, P<0.01). The expression levels of ß-catenin and SFRP1 were related to periodontal indexes. The correlation between the expression of SFRP1 and probing depth was most significant (r=0.723, P<0.01), as well as that between ß-catenin and bleeding index (r=0.697, P<0.01). CONCLUSIONS: Patients with CP exhibit elevated expression of SFRP1 and ß-catenin in gingival tissues, and this event is related to the degree of periodontal destruction. Abnormal expression of SFRP1 and ß-catenin may promote the development of periodontitis.

[Detection of Dickkopf-1 and alkaline phosphatase activity in gingival crevicular fluid from chronic periodontitis with Er:YAG laser as an adjunctive treatment].

PURPOSE: The aim of this study was to evaluate the clinical efficacy and the level of DKK1 and alkaline phosphatase (ALP) activity in gingival crevicular fluid (GCF) while taking Er:YAG laser as an adjunctive to scaling and root planning in the treatment of chronic periodontitis (CP). METHODS: Eleven patients with CP were included and there were nineteen pairs of homonym teeth(thirty-eight teeth) in this split-mouth design, and they were randomly assigned to experimental group or control group. In the experimental group, a combination of ultrasonic subgingigval scaling and root planning with hand instrument (SRP) were performed with Er: YAG laser as an adjunctive; in the control group, only SRP was performed. The main variables were bleeding index (BI), probing depth (PD), clinical attachment loss (CAL) which were assessed at baseline (1 week after ultrasonic subgingival scaling), l month and 3 months after treatment. GCF was collected at baseline, l week, l month and 3 months, and the levels of DKK1 and ALP activity were detected at the same time point. The data were analyzed with SPSS 19.0 software package. RESULTS: Both groups showed significant reduction of PD, CAL, BI values 1 month and 3months after treatment, but no significant difference in clinical parameters were found between the two groups. In the experimental group, the activity of ALP reduced to (386.69±146.42), (341.221±171.62), (249.27±98.72) from (396.191±150.55) U/L and the level of DKK1 dropped to (310.34±184.68), (270.04±55.14), (247.31±56.99) from (307.12±45.63) µg/L at the end of 1 week, 1 month, 3 months, respectively. Meanwhile, in the control group, the activity of ALP reduced to (374.72±131.27), (344.42±127.80), (252.36±90.4 ) from (394.09±120.25) U/L and the level of DKK1 dropped to (310.34±84.68), (270.04±55.14), (247.31±56.99) from (305.33±147.40) µg/L at the end of l week, l month, 3months, respectively. There is no significant difference between the two groups at any period for ALP or DKK1. CONCLUSIONS: Er:YAG laser was a safe no-surgical adjunctive therapy in treating chronic periodontitis, further observation is needed to determine its long-term effectiveness.

[Effect of periodontal initial therapy on the amount of SFRP1 in gingival crevicular fluid in patients with periodontitis].

PURPOSE: To detect the change of secreted frizzled-related protein-1（SFRP1） in gingival crevicular fluid during periodontal initial treatment and explore the relationship between SFRP1 and the activity of chronic periodontitis. METHODS: Twenty-two patients with moderate to severe periodontitis were selected, and 5 healthy volunteers were enrolled into the study as control group. The bleeding index(BI),periodontal probing depth(PD)and clinical attachment loss(CAL) were recorded at 1 week after supragingival scaling, one month after subgingival scaling. Gingival crevicular fluid (GCF) was collected at 1 week after supragingival scaling, one week after subgingival scaling, one month after subgingival scaling. The level of SFRP1 in GCF samples was detected by ELISA. SPSS19.0 software package was used for data processing. RESULTS: The amount of SFRP1 in GCF of moderate to severe periodontitis group was (40.80±4.85) pg, and that of normal control was (33.42±2.24) pg at 1 week after supragingival scaling. The amount of SFRP1 in GCF was significantly higher in moderate to severe periodontitis compared to normal control group (P<0.05). In moderate to severe periodontitis group, the amount of SFRP1 in GCF significantly increased at l week after subgingival scaling (45.99±5.23) pg compared to 1 week after supragingival scaling and 1 month after subgingival scaling (36.92±4.00) pg (P<0.05); There was significant decrease in the amount of SFRP1 in GCF at 1 month after subgingival scaling,compared to 1 week after supragingival scaling and l week after subgingival scaling (P<0.05). CONCLUSIONS: The BI, PD in the periodontitis groups were significantly improved at 1 month after initial therapy,and the amount of SFRP1 in GCF changed with different periodontal inflammation state．