ABSTRACT
BACKGROUND: The development of intraoperative sentinel node biopsy (SLNB) analysis methods, such as One-Step Nucleic Acid Amplification (OSNA), has made single-stage procedures possible. AIMS: We investigated the incidence of OSNA-detected lymph node positivity, comparing it to conventional histopathology, the incidence of non-SLNB node disease for the OSNA positive patients, and the breast re-operation rate, to assess the benefit from single-stage procedures. METHODS: This was a single-centre series of 573 consecutive patients undergoing SLNB (173 histopathology and 400 OSNA). RESULTS: OSNA-detected SLNB macrometastasis was similar to routine histopathology, with more micrometastasis detected (p < 0.001). Non-SLNB involvement in the OSNA group was similar to documented histopathological series. 27.6% of OSNA patients avoided further surgery because of OSNA. The median time for OSNA results was 42 min. CONCLUSIONS: OSNA is an effective method for detecting SLNB-metastasis. It is easily used in clinical practice, providing reliable results and negating the need for a second axillary operation.
Subject(s)
Nucleic Acid Amplification Techniques/methods , Sentinel Lymph Node Biopsy , Adult , Aged , Aged, 80 and over , Breast Neoplasms/pathology , Breast Neoplasms/surgery , Breast Neoplasms, Male/surgery , Female , Humans , Intraoperative Period , Male , Middle Aged , Reoperation/statistics & numerical dataSubject(s)
Biomarkers, Tumor/metabolism , Breast Neoplasms/diagnosis , Carcinoma, Ductal, Breast/diagnosis , Keratin-19/metabolism , Nucleic Acid Amplification Techniques , Sentinel Lymph Node Biopsy , Staining and Labeling/methods , Breast Neoplasms/metabolism , Carcinoma, Ductal, Breast/metabolism , Carcinoma, Ductal, Breast/secondary , Carcinoma, Intraductal, Noninfiltrating/metabolism , Carcinoma, Intraductal, Noninfiltrating/pathology , Female , Humans , Immunohistochemistry , Lymph Nodes/metabolism , Lymph Nodes/pathology , Lymphatic Metastasis , Neoplasm Micrometastasis/diagnosisABSTRACT
Diabetic mastopathy, or diabetic fibrous breast disease is a well-characterised benign, fibro-inflammatory condition affecting women with insulin dependent diabetes. To date the relationship between this condition and breast carcinoma has been poorly reported. We describe a case of breast carcinoma arising within a diabetic fibrous breast lesion, in a renal transplant recipient.
