ABSTRACT
Analysis of the pattern of extrachromosomal DNA in different cultures of Bacillus thuringiensis and Bacillus sphaericus demonstrated a higher content of extrachromosomal DNA in B. thuringiensis than in B. sphaericus. The quantity and approximate molecular weights of the plasmids were determined. The assumption that the plasmid DNA content in B. thuringiensis strains is higher than in the other representatives of the genus Bacillus was confirmed.
Subject(s)
Bacillus thuringiensis/genetics , Bacillus/genetics , DNA, Bacterial/genetics , Extrachromosomal Inheritance , Plasmids/genetics , Bacillus/classification , Bacillus thuringiensis/classification , Molecular Weight , Serotyping , Species SpecificitySubject(s)
Bacillus thuringiensis/genetics , DNA, Recombinant/genetics , Bacillus thuringiensis/growth & development , Bacillus thuringiensis Toxins , Bacterial Proteins/genetics , Bacterial Toxins/genetics , DNA, Recombinant/isolation & purification , Electrophoresis, Agar Gel , Endotoxins/genetics , Hemolysin Proteins , Species Specificity , Spectrophotometry, UltravioletABSTRACT
The extrachromosomal DNAs from different strains of Bacillus thuringiensis subsp. israelensis were comparatively analysed. Within the serotype studied, two groups of strains have been revealed. These are characterised by a certain plasmid composition as well as by specific physiological, biochemical and insecticide properties.
