ABSTRACT
Se cultivó el hemoflagelado Crithidia luciliae con el objetivo de desarrollar un método para la determinación de anticuerpos anti-DNA de doble cadena (dc) séricos. Se prepararon láminas portaobjetos, que fueron utilizadas para la detección de estos anticuerpos por el método de inmunofluorescencia indirecta en 124 muestras de suero de pacientes con enfermedades autoinmunes reumáticas. Se compararon los resultados de las preparaciones con los obtenidos sobre láminas comerciales. Solo 4 muestras fueron discordantes, y se obtuvo un coeficiente Kappa de 0.92. Estos resultados demostraron el valor diagnóstico de estas preparaciones para la detección de anticuerpos anti-DNAdc(AU)
Subject(s)
Crithidia , Antibodies, Antinuclear , Fluorescent Antibody Technique, IndirectABSTRACT
Human samples of articular cartilage from the knee of a clinically classified osteoarthritic patient, assessed by arthroscopy as part of the surgical treatment was studied by light and transmission electron microscopy. This particular case differed from others already reported in the variability of cell phenotype within the aggregates or "clones" frequently present in the osteoarthritic cartilage. The most common morphology of "clonal" cells forming the aggregates were large and rounded with an euchromatic nucleus. The cytoplasm was characterized by the presence of alternately clear and dense sites. At the ultrastructural level it was seen that the clear sites were formed by disrupted intermediates filaments and small particles, and that the dense sites were constituted by the segregation of different organelles of the chondrocytes. In addition, there were atypical aggregates composed only by secretory cells or by degenerating chondrocytes. Furthermore, a complex structure consisting of a very large cell inside a giant lacunae delimited by electron-dense material with small vesicles is described as a novel finding. The variability in the chondrocyte phenotype of the aggregates described here could be an indication of a better prognosis; nevertheless, the follow-up of the evolution of this patient is needed in order to know the final outcome.
Subject(s)
Humans , Cartilage, Articular , Osteoarthritis , Cell Aggregation , Cell Movement , Clone Cells/pathology , PhenotypeABSTRACT
Human samples of articular cartilage from the knee of a clinically classified osteoarthritic patient, assessed by arthroscopy as part of the surgical treatment was studied by light and transmission electron microscopy. This particular case differed from others already reported in the variability of cell phenotype within the aggregates or "clones" frequently present in the osteoarthritic cartilage. The most common morphology of "clonal" cells forming the aggregates were large and rounded with an euchromatic nucleus. The cytoplasm was characterized by the presence of alternately clear and dense sites. At the ultrastructural level it was seen that the clear sites were formed by disrupted intermediates filaments and small particles, and that the dense sites were constituted by the segregation of different organelles of the chondrocytes. In addition, there were atypical aggregates composed only by secretory cells or by degenerating chondrocytes. Furthermore, a complex structure consisting of a very large cell inside a giant lacunae delimited by electron-dense material with small vesicles is described as a novel finding. The variability in the chondrocyte phenotype of the aggregates described here could be an indication of a better prognosis; nevertheless, the follow-up of the evolution of this patient is needed in order to know the final outcome.(AU)
