Subject(s)
Polymerase Chain Reaction/methods , Animals , Bacterial Proteins/genetics , Biotechnology , Cattle , DNA Primers , DNA, Bacterial/genetics , DNA, Viral/genetics , Evaluation Studies as Topic , Female , Humans , Male , Papillomaviridae/genetics , Sex Determination Analysis/methods , Staphylococcus aureus/genetics , Y Chromosome/geneticsABSTRACT
The major components of protein extracts from the cattle tick Boophilus microplus eggs and larvae of various ages were characterized by molecular sieving chromatography, ion exchange chromatography and SDS-PAGE. The fractions analysed showed a changing chromatographic pattern development. A serum raised against the components of a fraction showing characteristics of vitellin strongly reacted in Western blots with the major peptides of extracts from eggs, larvae, gut and ovary. Comparison of patterns obtained by electrophoresis in non-denaturing PAGE, stained with Coomassie blue or with benzidine/hydrogen peroxide, revealed that the major proteins of these extracts are haemoproteins, possibly in different aggregation states or heterogeneous in composition.
Subject(s)
Egg Proteins/metabolism , Ticks/metabolism , Animals , Cattle , Chromatography, Ion Exchange , Digestive System , Female , Larva , Ovum/metabolism , Peptides/metabolism , Ticks/growth & developmentABSTRACT
A entrada de animais infestados por carrapatos nas regiöes livres, nos meses de veräo, provoca surtos de tristeza parasitária nos animais nativos, que normalmente se encontram sem proteçäo para esta enfermidade. Nos veröes de 1987 e 1988, ocorreram vários focos isolados de carrapatos em Santa Vitória do Palmar, regiäo indene do extremo sul do Brasil. O presente trabalho teve como objetivo colaborar no esclarecimento do assunto, chegando os autores à conclusäo de que o carrapato ali existente é o Boophilus microplus, naturalmente livre de Babesia spp
