ABSTRACT
Human α-defensin 5 (HD5) is a 32-residue cysteine-rich host-defense peptide that exhibits broad-spectrum antimicrobial activity and contributes to innate immunity in the human gut and other organ systems. Despite many years of investigation, its antimicrobial mechanism of action remains unclear. In this work, we report that HD5ox, the oxidized form of this peptide that exhibits three regiospecific disulfide bonds, causes distinct morphological changes to Escherichia coli and other Gram-negative microbes. These morphologies include bleb formation, cellular elongation, and clumping. The blebs are up to â¼1 µm wide and typically form at the site of cell division or cell poles. Studies with E. coli expressing cytoplasmic GFP reveal that HD5ox treatment causes GFP emission to localize in the bleb. To probe the cellular uptake of HD5ox and subsequent localization, we describe the design and characterization of a fluorophore-HD5 conjugate family. By employing these peptides, we demonstrate that fluorophore-HD5ox conjugates harboring the rhodamine and coumarin fluorophores enter the E. coli cytoplasm. On the basis of the fluorescence profiles, each of these fluorophore-HD5ox conjugates localizes to the site of cell division and cell poles. These studies support the notion that HD5ox, at least in part, exerts its antibacterial activity against E. coli and other Gram-negative microbes in the cytoplasm.
Subject(s)
Anti-Bacterial Agents/pharmacology , Escherichia coli/drug effects , alpha-Defensins/pharmacology , Amino Acid Sequence , Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/metabolism , Disulfides/chemistry , Disulfides/pharmacology , Escherichia coli/cytology , Gram-Negative Bacteria/cytology , Gram-Negative Bacteria/drug effects , Humans , Microbial Sensitivity Tests , Microbial Viability/drug effects , Microscopy, Electron, Scanning/methods , Molecular Sequence Data , Oxidation-Reduction , alpha-Defensins/chemistry , alpha-Defensins/metabolismABSTRACT
Defensin attack! Here we report the screening of human defensin 5 against the Keio Collection of E. coli strains. The results of this screen further our understanding of how this important hostdefense peptide kills bacteria and how bacteria protect themselves against the attack from the human host.
Subject(s)
Escherichia coli Infections/immunology , Escherichia coli/physiology , Host-Pathogen Interactions , Mutation , alpha-Defensins/immunology , Amino Acid Sequence , Escherichia coli/genetics , Escherichia coli/immunology , Escherichia coli Infections/microbiology , Gene Library , Humans , Immunity, Innate , Models, Molecular , Molecular Sequence Data , alpha-Defensins/chemistryABSTRACT
We report the discovery of HD5-CD, an unprecedented C2-symmetric ß-barrel-like covalent dimer of the cysteine-rich host-defense peptide human defensin 5 (HD5). Dimerization results from intermonomer disulfide exchange between the canonical α-defensin Cys(II)-Cys(IV) (Cys(5)-Cys(20)) bonds located at the hydrophobic interface. This disulfide-locked dimeric assembly provides a new element of structural diversity for cysteine-rich peptides as well as increased protease resistance, broad-spectrum antimicrobial activity, and enhanced potency against the opportunistic human pathogen Acinetobacter baumannii.
