ABSTRACT
By means of fluorescent method biogenic amines in the thymus tissue basophils have been investigated in intact and immunized rats and, simultaneously, in the peripheral blood of the immunized animals the hemolysins titer has been defined as an index of the immune response of the organism. In the immunized rats the number of the tissue basophils is increasing, there are many degranulated forms among them. Hence, the antigenic stimulation facilitates to the tissue basophils secretory activation, which is morphologically manifested as their degranulation. The hemolysins titer level in the peripheral blood is gradually decreasing, as the time of immunization increases. The content of biogenic amines in the tissue basophils does not undergo similar changes.
Subject(s)
Hemolysin Proteins/biosynthesis , Histamine/metabolism , Mast Cells/metabolism , Serotonin/metabolism , Thymus Gland/cytology , Animals , Cytoplasmic Granules/ultrastructure , Female , Microscopy, Fluorescence , Rats , Thymus Gland/immunology , Time FactorsABSTRACT
T- and B-populations of lymphocytes from the thymus, spleen and mesenteric lymph nodes have been studied. Rabbits of various age were immunized intravenously with suspension of 20 . 10 g/kg sheep erythrocytes. The results of the histochemical and scanning electron microscopic investigation demonstrate that in 6-month-old animals B-lymphocytes transform intensively into plasmic cells. Under antigenic stimulation, certain changes of the lymphatic surface are demonstrated. Enzymatic activity specific for T- and B-depending zones is stated.