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1.
Cell Rep ; 43(6): 114267, 2024 Jun 25.
Article in English | MEDLINE | ID: mdl-38795344

ABSTRACT

In the adult brain, structural and functional parameters, such as synaptic sizes and neuronal firing rates, follow right-skewed and heavy-tailed distributions. While this organization is thought to have significant implications, its development is still largely unknown. Here, we address this knowledge gap by investigating a large-scale dataset recorded from the prefrontal cortex and the olfactory bulb of mice aged 4-60 postnatal days. We show that firing rates and spike train interactions have a largely stable distribution shape throughout the first 60 postnatal days and that the prefrontal cortex displays a functional small-world architecture. Moreover, early brain activity exhibits an oligarchical organization, where high-firing neurons have hub-like properties. In a neural network model, we show that analogously right-skewed and heavy-tailed synaptic parameters are instrumental to consistently recapitulate the experimental data. Thus, functional and structural parameters in the developing brain are already extremely distributed, suggesting that this organization is preconfigured and not experience dependent.


Subject(s)
Brain , Animals , Mice , Brain/growth & development , Olfactory Bulb/growth & development , Neurons/metabolism , Mice, Inbred C57BL , Synapses/metabolism , Synapses/physiology , Prefrontal Cortex/growth & development , Prefrontal Cortex/cytology , Action Potentials/physiology , Nerve Net/growth & development , Models, Neurological
2.
Nat Commun ; 15(1): 738, 2024 Jan 25.
Article in English | MEDLINE | ID: mdl-38272901

ABSTRACT

Sharp wave-ripples (SPW-Rs) are a hippocampal network phenomenon critical for memory consolidation and planning. SPW-Rs have been extensively studied in the adult brain, yet their developmental trajectory is poorly understood. While SPWs have been recorded in rodents shortly after birth, the time point and mechanisms of ripple emergence are still unclear. Here, we combine in vivo electrophysiology with optogenetics and chemogenetics in 4 to 12-day-old mice to address this knowledge gap. We show that ripples are robustly detected and induced by light stimulation of channelrhodopsin-2-transfected CA1 pyramidal neurons only from postnatal day 10 onwards. Leveraging a spiking neural network model, we mechanistically link the maturation of inhibition and ripple emergence. We corroborate these findings by reducing ripple rate upon chemogenetic silencing of CA1 interneurons. Finally, we show that early SPW-Rs elicit a more robust prefrontal cortex response than SPWs lacking ripples. Thus, development of inhibition promotes ripples emergence.


Subject(s)
Hippocampus , Pyramidal Cells , Mice , Animals , Hippocampus/physiology , Pyramidal Cells/physiology , Interneurons/physiology
3.
bioRxiv ; 2023 Dec 30.
Article in English | MEDLINE | ID: mdl-38234832

ABSTRACT

Neuronal firing sequences are thought to be the basic building blocks of neural coding and information broadcasting within the brain. However, when sequences emerge during neurodevelopment remains unknown. We demonstrate that structured firing sequences are present in spontaneous activity of human brain organoids and ex vivo neonatal brain slices from the murine somatosensory cortex. We observed a balance between temporally rigid and flexible firing patterns that are emergent phenomena in human brain organoids and early postnatal murine somatosensory cortex, but not in primary dissociated cortical cultures. Our findings suggest that temporal sequences do not arise in an experience-dependent manner, but are rather constrained by an innate preconfigured architecture established during neurogenesis. These findings highlight the potential for brain organoids to further explore how exogenous inputs can be used to refine neuronal circuits and enable new studies into the genetic mechanisms that govern assembly of functional circuitry during early human brain development.

4.
Elife ; 112022 08 17.
Article in English | MEDLINE | ID: mdl-35975980

ABSTRACT

Throughout development, the brain transits from early highly synchronous activity patterns to a mature state with sparse and decorrelated neural activity, yet the mechanisms underlying this process are poorly understood. The developmental transition has important functional consequences, as the latter state is thought to allow for more efficient storage, retrieval, and processing of information. Here, we show that, in the mouse medial prefrontal cortex (mPFC), neural activity during the first two postnatal weeks decorrelates following specific spatial patterns. This process is accompanied by a concomitant tilting of excitation-inhibition (E-I) ratio toward inhibition. Using optogenetic manipulations and neural network modeling, we show that the two phenomena are mechanistically linked, and that a relative increase of inhibition drives the decorrelation of neural activity. Accordingly, in mice mimicking the etiology of neurodevelopmental disorders, subtle alterations in E-I ratio are associated with specific impairments in the correlational structure of spike trains. Finally, capitalizing on EEG data from newborn babies, we show that an analogous developmental transition takes place also in the human brain. Thus, changes in E-I ratio control the (de)correlation of neural activity and, by these means, its developmental imbalance might contribute to the pathogenesis of neurodevelopmental disorders.


Subject(s)
Neurodevelopmental Disorders , Prefrontal Cortex , Animals , Brain , Humans , Inhibition, Psychological , Mice , Neural Inhibition , Prefrontal Cortex/physiology
5.
Nat Commun ; 13(1): 4571, 2022 08 05.
Article in English | MEDLINE | ID: mdl-35931682

ABSTRACT

Life-long brain function and mental health are critically determined by developmental processes occurring before birth. During mammalian pregnancy, maternal cells are transferred to the fetus. They are referred to as maternal microchimeric cells (MMc). Among other organs, MMc seed into the fetal brain, where their function is unknown. Here, we show that, in the offspring's developing brain in mice, MMc express a unique signature of sensome markers, control microglia homeostasis and prevent excessive presynaptic elimination. Further, MMc facilitate the oscillatory entrainment of developing prefrontal-hippocampal circuits and support the maturation of behavioral abilities. Our findings highlight that MMc are not a mere placental leak out, but rather a functional mechanism that shapes optimal conditions for healthy brain function later in life.


Subject(s)
Chimerism , Maternal-Fetal Exchange , Animals , Female , Fetus , Mammals , Mice , Parturition , Placenta , Pregnancy
6.
Sci Data ; 9(1): 113, 2022 03 29.
Article in English | MEDLINE | ID: mdl-35351935

ABSTRACT

The acute effects of anesthesia and their underlying mechanisms are still not fully understood. Thus, comprehensive analysis and efficient generalization require their description in various brain regions. Here we describe a large-scale, annotated collection of 2-photon calcium imaging data and multi-electrode, extracellular electrophysiological recordings in CA1 of the murine hippocampus under three distinct anesthetics (Isoflurane, Ketamine/Xylazine and Medetomidine/Midazolam/Fentanyl), during natural sleep, and wakefulness. We cover several aspects of data quality standardization and provide a set of tools for autonomous validation, along with analysis workflows for reuse and data exploration. The datasets described here capture various aspects of neural activity in hundreds of pyramidal cells at single cell resolution. In addition to relevance for basic biological research, the dataset may find utility in computational neuroscience as a benchmark for models of anesthesia and sleep.


Subject(s)
Anesthesia , Calcium , Hippocampus , Sleep , Animals , Hippocampus/physiology , Mice , Xylazine
7.
PLoS Biol ; 19(4): e3001146, 2021 04.
Article in English | MEDLINE | ID: mdl-33793545

ABSTRACT

General anesthesia is characterized by reversible loss of consciousness accompanied by transient amnesia. Yet, long-term memory impairment is an undesirable side effect. How different types of general anesthetics (GAs) affect the hippocampus, a brain region central to memory formation and consolidation, is poorly understood. Using extracellular recordings, chronic 2-photon imaging, and behavioral analysis, we monitor the effects of isoflurane (Iso), medetomidine/midazolam/fentanyl (MMF), and ketamine/xylazine (Keta/Xyl) on network activity and structural spine dynamics in the hippocampal CA1 area of adult mice. GAs robustly reduced spiking activity, decorrelated cellular ensembles, albeit with distinct activity signatures, and altered spine dynamics. CA1 network activity under all 3 anesthetics was different to natural sleep. Iso anesthesia most closely resembled unperturbed activity during wakefulness and sleep, and network alterations recovered more readily than with Keta/Xyl and MMF. Correspondingly, memory consolidation was impaired after exposure to Keta/Xyl and MMF, but not Iso. Thus, different anesthetics distinctly alter hippocampal network dynamics, synaptic connectivity, and memory consolidation, with implications for GA strategy appraisal in animal research and clinical settings.


Subject(s)
Anesthetics/adverse effects , Hippocampus/drug effects , Memory Consolidation/drug effects , Spine/drug effects , Anesthesia/adverse effects , Anesthetics/pharmacology , Animals , Electrophysiological Phenomena/drug effects , Female , Fentanyl/adverse effects , Fentanyl/pharmacology , Hippocampus/cytology , Hippocampus/physiology , Isoflurane/adverse effects , Isoflurane/pharmacology , Ketamine/adverse effects , Ketamine/pharmacology , Male , Medetomidine/adverse effects , Medetomidine/pharmacology , Memory Disorders/chemically induced , Memory Disorders/pathology , Mice , Mice, Inbred C57BL , Mice, Transgenic , Midazolam/adverse effects , Midazolam/pharmacology , Nerve Net/drug effects , Nerve Net/physiology , Spine/physiology , Xylazine/adverse effects , Xylazine/pharmacology
8.
Neuron ; 109(8): 1350-1364.e6, 2021 04 21.
Article in English | MEDLINE | ID: mdl-33675685

ABSTRACT

Disturbed neuronal activity in neuropsychiatric pathologies emerges during development and might cause multifold neuronal dysfunction by interfering with apoptosis, dendritic growth, and synapse formation. However, how altered electrical activity early in life affects neuronal function and behavior in adults is unknown. Here, we address this question by transiently increasing the coordinated activity of layer 2/3 pyramidal neurons in the medial prefrontal cortex of neonatal mice and monitoring long-term functional and behavioral consequences. We show that increased activity during early development causes premature maturation of pyramidal neurons and affects interneuronal density. Consequently, altered inhibitory feedback by fast-spiking interneurons and excitation/inhibition imbalance in prefrontal circuits of young adults result in weaker evoked synchronization of gamma frequency. These structural and functional changes ultimately lead to poorer mnemonic and social abilities. Thus, prefrontal activity during early development actively controls the cognitive performance of adults and might be critical for cognitive symptoms in neuropsychiatric diseases.


Subject(s)
Cognitive Dysfunction/physiopathology , Cortical Synchronization/physiology , Nerve Net/physiopathology , Neurons/physiology , Animals , Electric Stimulation , Inhibitory Postsynaptic Potentials/physiology , Mice , Nerve Net/growth & development , Optogenetics
9.
Trends Neurosci ; 44(3): 227-240, 2021 03.
Article in English | MEDLINE | ID: mdl-33246578

ABSTRACT

The role of the prefrontal cortex (PFC) takes center stage among unanswered questions in modern neuroscience. The PFC has a Janus-faced nature: it enables sophisticated cognitive and social abilities that reach their maximum expression in humans, yet it underlies some of the devastating symptoms of psychiatric disorders. Accordingly, appropriate prefrontal development is crucial for many high-order cognitive abilities and dysregulation of this process has been linked to various neuropsychiatric diseases. Reviewing recent advances in the field, with a primary focus on rodents and humans, we highlight why, despite differences across species, a cross-species approach is a fruitful strategy for understanding prefrontal development. We briefly review the developmental contribution of molecules and extensively discuss how electrical activity controls the early maturation and wiring of prefrontal areas, as well as the emergence and refinement of input-output circuitry involved in cognitive processing. Finally, we highlight the mechanisms of developmental dysfunction and their relevance for psychiatric disorders.


Subject(s)
Mental Disorders , Rodentia , Animals , Humans , Prefrontal Cortex
10.
Neuron ; 105(1): 60-74.e7, 2020 01 08.
Article in English | MEDLINE | ID: mdl-31733940

ABSTRACT

Cognitive deficits, core features of mental illness, largely result from dysfunction of prefrontal networks. This dysfunction emerges during early development, before a detectable behavioral readout, yet the cellular elements controlling the abnormal maturation are still unknown. Here, we address this open question by combining in vivo electrophysiology, optogenetics, neuroanatomy, and behavioral assays during development in mice mimicking the dual genetic-environmental etiology of psychiatric disorders. We report that pyramidal neurons in superficial layers of the prefrontal cortex are key elements causing disorganized oscillatory entrainment of local circuits in beta-gamma frequencies. Their abnormal firing rate and timing relate to sparser dendritic arborization and lower spine density. Administration of minocycline during the first postnatal week, potentially acting via microglial cells, rescues the neuronal deficits and restores pre-juvenile cognitive abilities. Elucidation of the cellular substrate of developmental miswiring causing later cognitive deficits opens new perspectives for identification of neurobiological targets amenable to therapies.


Subject(s)
Cognitive Dysfunction/prevention & control , Cognitive Dysfunction/physiopathology , Microglia/physiology , Minocycline/pharmacology , Prefrontal Cortex/physiology , Pyramidal Cells/physiology , Animals , Animals, Newborn , Atrophy/pathology , Behavior, Animal/physiology , Beta Rhythm/physiology , Cognitive Dysfunction/genetics , Cognitive Dysfunction/pathology , Dendrites/pathology , Dendritic Spines/pathology , Female , Gamma Rhythm/physiology , Male , Mice , Mutation , Nerve Tissue Proteins/genetics , Neural Pathways/physiopathology , Optogenetics , Poly I-C , Prefrontal Cortex/pathology
11.
Front Neural Circuits ; 13: 38, 2019.
Article in English | MEDLINE | ID: mdl-31191258

ABSTRACT

Monitoring the hypnotic component of anesthesia during surgeries is critical to prevent intraoperative awareness and reduce adverse side effects. For this purpose, electroencephalographic (EEG) methods complementing measures of autonomic functions and behavioral responses are in use in clinical practice. However, in human neonates and infants existing methods may be unreliable and the correlation between brain activity and anesthetic depth is still poorly understood. Here, we characterized the effects of different anesthetics on brain activity in neonatal mice and developed machine learning approaches to identify electrophysiological features predicting inspired or end-tidal anesthetic concentration as a proxy for anesthetic depth. We show that similar features from EEG recordings can be applied to predict anesthetic concentration in neonatal mice and humans. These results might support a novel strategy to monitor anesthetic depth in human newborns.


Subject(s)
Algorithms , Anesthesia , Anesthetics/pharmacology , Brain/drug effects , Animals , Animals, Newborn , Brain/physiology , Electroencephalography , Female , Humans , Infant , Infant, Newborn , Machine Learning , Male , Mice , Mice, Inbred C57BL
12.
Eur J Neurosci ; 50(6): 3072-3084, 2019 09.
Article in English | MEDLINE | ID: mdl-31087437

ABSTRACT

Despite inherent difficulties to translate human cognitive phenotype into animals, a large number of animal models for psychiatric disorders, such as schizophrenia, have been developed over the last decades. To which extent they reproduce common patterns of dysfunction related to mental illness and abnormal processes of maturation is still largely unknown. While the devastating symptoms of disease are firstly detectable in adulthood, they are considered to reflect profound miswiring of brain circuitry as result of abnormal development. To reveal whether different disease models share common dysfunction early in life, we investigate the prefrontal-hippocampal communication at neonatal age in (a) mice mimicking the abnormal genetic background (22q11.2 microdeletion, DISC1 knockdown), (b) mice mimicking the challenge by environmental stressors (maternal immune activation during pregnancy), (c) mice mimicking the combination of both aetiologies (dual-hit models) and pharmacological mouse models. Simultaneous extracellular recordings in vivo from all layers of prelimbic subdivision (PL) of prefrontal cortex (PFC) and CA1 area of intermediate/ventral hippocampus (i/vHP) show that network oscillations have a more fragmented structure and decreased power mainly in neonatal mice that mimic both genetic and environmental aetiology of disease. These mice also show layer-specific firing deficits in PL. Similar early network dysfunction was present in mice with 22q11.2 microdeletion. The abnormal activity patterns are accompanied by weaker synchrony and directed interactions within prefrontal-hippocampal networks. Thus, only severe genetic defects or combined genetic environmental stressors are disruptive enough for reproducing the early network miswiring in mental disorders.


Subject(s)
Hippocampus/physiopathology , Mental Disorders/physiopathology , Nerve Net/physiopathology , Neurons/physiology , Prefrontal Cortex/physiopathology , Action Potentials/physiology , Animals , Behavior, Animal/physiology , Disease Models, Animal , Hippocampus/growth & development , Mice , Nerve Net/growth & development , Prefrontal Cortex/growth & development
13.
J Neurosci ; 39(7): 1222-1235, 2019 02 13.
Article in English | MEDLINE | ID: mdl-30617212

ABSTRACT

Compromised brain development has been hypothesized to account for mental illness. This concept was underpinned by the function of the molecule disrupted-in-schizophrenia 1 (DISC1), which represents an intracellular hub of developmental processes and has been related to cognitive dysfunction in psychiatric disorders. Mice with whole-brain DISC1 knock-down show impaired prefrontal-hippocampal function and cognitive abilities throughout development and at adulthood, especially when combined with early environmental stressors, such as maternal immune activation (MIA). However, the contribution of abnormal DISC1-driven maturation of either prefrontal cortex (PFC) or hippocampus (HP) to these deficits is still unknown. Here, we use in utero electroporation to restrict the DISC1 knock-down to prefrontal layer II/III pyramidal neurons during perinatal development and expose these mice to MIA as an environmental stressor (dual-hit GPFCE mice, both sexes). Combining in vivo electrophysiology and neuroanatomy with behavioral testing, we show that GPFCE mice at neonatal age have abnormal patterns of oscillatory activity and firing in PFC, but not HP. Abnormal firing rates in PFC of GPFCE mice relate to sparser dendritic arborization and lower spine density. Moreover, the long-range coupling within prefrontal-hippocampal networks is decreased at this age. The transient prefrontal DISC1 knock-down was sufficient to permanently perturb the prefrontal-hippocampal communication and caused poorer recognition memory performance at pre-juvenile age. Thus, developmental dysfunction of prefrontal circuitry causes long-lasting disturbances related to mental illness.SIGNIFICANCE STATEMENT Hypofrontality is considered a main cause of cognitive deficits in mental disorders, yet the underlying mechanisms are still largely unknown. During development, long before the emergence of disease symptoms, the functional coupling within the prefrontal-hippocampal network, which is the core brain circuit involved in cognitive processing, is reduced. To assess to which extent impaired prefrontal development contributes to the early dysfunction, immune-challenged mice with transient DISC1 knock-down confined to PFC were investigated in their prefrontal-hippocampal communication throughout development by in vivo electrophysiology and behavioral testing. We show that perturbing developmental processes of prefrontal layer II/III pyramidal neurons is sufficient to diminish prefrontal-hippocampal coupling and decrease the cognitive performance throughout development.


Subject(s)
Cognitive Dysfunction/genetics , Nerve Tissue Proteins/genetics , Animals , Behavior, Animal/physiology , Cognitive Dysfunction/psychology , Exploratory Behavior/physiology , Female , Gene Knockdown Techniques , Hippocampus/cytology , Hippocampus/growth & development , Mice , Mice, Inbred C57BL , Neural Pathways/growth & development , Prefrontal Cortex/cytology , Prefrontal Cortex/growth & development , Pregnancy , Prenatal Exposure Delayed Effects , Pyramidal Cells/ultrastructure , Recognition, Psychology/physiology
14.
Elife ; 72018 04 10.
Article in English | MEDLINE | ID: mdl-29631696

ABSTRACT

The long-range coupling within prefrontal-hippocampal networks that account for cognitive performance emerges early in life. The discontinuous hippocampal theta bursts have been proposed to drive the generation of neonatal prefrontal oscillations, yet the cellular substrate of these early interactions is still unresolved. Here, we selectively target optogenetic manipulation of glutamatergic projection neurons in the CA1 area of either dorsal or intermediate/ventral hippocampus at neonatal age to elucidate their contribution to the emergence of prefrontal oscillatory entrainment. We show that despite stronger theta and ripples power in dorsal hippocampus, the prefrontal cortex is mainly coupled with intermediate/ventral hippocampus by phase-locking of neuronal firing via dense direct axonal projections. Theta band-confined activation by light of pyramidal neurons in intermediate/ventral but not dorsal CA1 that were transfected by in utero electroporation with high-efficiency channelrhodopsin boosts prefrontal oscillations. Our data causally elucidate the cellular origin of the long-range coupling in the developing brain.


Subject(s)
Glutamic Acid/metabolism , Hippocampus/physiology , Interneurons/physiology , Neural Pathways/physiology , Prefrontal Cortex/physiology , Pyramidal Cells/physiology , Theta Rhythm , Action Potentials , Animals , Animals, Newborn , Female , Male , Mice , Mice, Inbred C57BL , Optogenetics , Receptors, Glutamate/metabolism
15.
J Neurosci ; 37(21): 5263-5273, 2017 05 24.
Article in English | MEDLINE | ID: mdl-28539349

ABSTRACT

We previously found that Mertk and its ligand Gas6, astrocytic genes involved in phagocytosis, are upregulated after acute sleep deprivation. These results suggested that astrocytes may engage in phagocytic activity during extended wake, but direct evidence was lacking. Studies in humans and rodents also found that sleep loss increases peripheral markers of inflammation, but whether these changes are associated with neuroinflammation and/or activation of microglia, the brain's resident innate immune cells, was unknown. Here we used serial block-face scanning electron microscopy to obtain 3D volume measurements of synapses and surrounding astrocytic processes in mouse frontal cortex after 6-8 h of sleep, spontaneous wake, or sleep deprivation (SD) and after chronic (∼5 d) sleep restriction (CSR). Astrocytic phagocytosis, mainly of presynaptic components of large synapses, increased after both acute and chronic sleep loss relative to sleep and wake. MERTK expression and lipid peroxidation in synaptoneurosomes also increased to a similar extent after short and long sleep loss, suggesting that astrocytic phagocytosis may represent the brain's response to the increase in synaptic activity associated with prolonged wake, clearing worn components of heavily used synapses. Using confocal microscopy, we then found that CSR but not SD mice show morphological signs of microglial activation and enhanced microglial phagocytosis of synaptic elements, without obvious signs of neuroinflammation in the CSF. Because low-level sustained microglia activation can lead to abnormal responses to a secondary insult, these results suggest that chronic sleep loss, through microglia priming, may predispose the brain to further damage.SIGNIFICANCE STATEMENT We find that astrocytic phagocytosis of synaptic elements, mostly of presynaptic origin and in large synapses, is upregulated already after a few hours of sleep deprivation and shows a further significant increase after prolonged and severe sleep loss, suggesting that it may promote the housekeeping of heavily used and strong synapses in response to the increased neuronal activity of extended wake. By contrast, chronic sleep restriction but not acute sleep loss activates microglia, promotes their phagocytic activity, and does so in the absence of overt signs of neuroinflammation, suggesting that like many other stressors, extended sleep disruption may lead to a state of sustained microglia activation, perhaps increasing the brain's susceptibility to other forms of damage.


Subject(s)
Astrocytes/metabolism , Cerebral Cortex/metabolism , Microglia/metabolism , Phagocytosis , Sleep Deprivation/metabolism , Animals , Cerebral Cortex/cytology , Cerebral Cortex/physiopathology , Female , Intercellular Signaling Peptides and Proteins/genetics , Intercellular Signaling Peptides and Proteins/metabolism , Male , Mice , Mice, Inbred C57BL , Proto-Oncogene Proteins/genetics , Proto-Oncogene Proteins/metabolism , Receptor Protein-Tyrosine Kinases/genetics , Receptor Protein-Tyrosine Kinases/metabolism , Synapses/metabolism , c-Mer Tyrosine Kinase
16.
Sci Rep ; 6: 36804, 2016 11 10.
Article in English | MEDLINE | ID: mdl-27830758

ABSTRACT

Exploration of a novel environment leads to neuronal DNA double-strand breaks (DSBs). These DSBs are generated by type 2 topoisomerase to relieve topological constrains that limit transcription of plasticity-related immediate early genes. If not promptly repaired, however, DSBs may lead to cell death. Since the induction of plasticity-related genes is higher in wake than in sleep, we asked whether it is specifically wake associated with synaptic plasticity that leads to DSBs, and whether sleep provides any selective advantage over wake in their repair. In flies and mice, we find that enriched wake, more than simply time spent awake, induces DSBs, and their repair in mice is delayed or prevented by subsequent wake. In both species the repair of irradiation-induced neuronal DSBs is also quicker during sleep, and mouse genes mediating the response to DNA damage are upregulated in sleep. Thus, sleep facilitates the repair of neuronal DSBs.


Subject(s)
DNA Breaks, Double-Stranded , DNA Repair , Neurons/physiology , Sleep , Animals , Brain/pathology , Brain/radiation effects , DNA Repair Enzymes/genetics , DNA Repair Enzymes/metabolism , Drosophila melanogaster , Female , Male , Mice, Inbred C57BL , Neurons/radiation effects , Radiation Injuries, Experimental/genetics , Transcriptional Activation
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