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1.
J Chromatogr ; 446: 179-85, 1988 Jul 27.
Article in English | MEDLINE | ID: mdl-3209654

ABSTRACT

A preparative-scale enrichment of the allergenic components of the pollen extract of Parietaria judaica, which grow all over the Mediterranean area, has been obtained by high-performance liquid chromatography, operating in the ion-exchange mode at pH 7 with a curvilinear ionic-strength gradient.


Subject(s)
Allergens/analysis , Plant Extracts/analysis , Pollen/analysis , Chromatography, High Pressure Liquid , Isoelectric Focusing , Radioallergosorbent Test , Ultrafiltration
2.
J Chromatogr ; 444: 209-18, 1988 Jul 01.
Article in English | MEDLINE | ID: mdl-3204131

ABSTRACT

Size-exclusion and ion-exchange high-performance liquid chromatography were used to monitor the presence of polymeric aggregates and of families of allergens of similar isoelectric point in Parietaria judaica pollen extracts. A radio-allergo-sorbent test and chromatofocusing were used for the analysis of individual fractions. This allows the detection of two groups of allergens, having pI of 4.7 and greater than 5.7, respectively, and shows the effect of dialysis or ultrafiltration on the distribution of allergens.


Subject(s)
Allergens/analysis , Plant Extracts/analysis , Pollen/analysis , Chromatography, Gel , Chromatography, High Pressure Liquid , Evaluation Studies as Topic , Isoelectric Focusing , Radioallergosorbent Test
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