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1.
Gene Ther ; 22(8): 619-27, 2015 Aug.
Article in English | MEDLINE | ID: mdl-25965394

ABSTRACT

Loss of SPATA7 function causes the pathogenesis of Leber congenital amaurosis and retinitis pigmentosa. Spata7 knockout mice mimic human SPATA7-related retinal disease with apparent photoreceptor degeneration observed as early as postnatal day 15 (P15). To test the efficacy of adeno-associated virus (AAV)-mediated gene therapy for rescue of photoreceptor survival and function in Spata7 mutant mice, we employed the AAV8(Y733F) vector carrying hGRK1-driven full-length FLAG-tagged Spata7 cDNA to target both rod and cone photoreceptors. Following subretinal injection of this vector, FLAG-tagged SPATA7 was found to colocalize with endogenous SPATA7 in wild-type mice. In Spata7 mutant mice initially treated at P15, we observed improvement of photoresponse, photoreceptor ultrastructure and significant alleviation of photoreceptor degeneration. Furthermore, we performed treatments at P28 and P56 and found that all treatments (P15-P56) can ameliorate rod and cone loss in the long term (1 year); however, none efficiently protect photoreceptors from degeneration by 86 weeks of age as only a small amount of treated photoreceptors can survive to this time. This study demonstrates long-term improvement of photoreceptor function by AAV8(Y733F)-introduced Spata7 expression in a mouse model as potential treatment of the human disease, but also suggests that treated mutant photoreceptors still undergo progressive degeneration.


Subject(s)
DNA-Binding Proteins/genetics , Dependovirus/genetics , Genetic Therapy , Leber Congenital Amaurosis/therapy , Retinitis Pigmentosa/therapy , Animals , Dependovirus/metabolism , Disease Models, Animal , Genetic Vectors , Leber Congenital Amaurosis/genetics , Mice, Inbred C57BL , Mice, Knockout , Retinal Cone Photoreceptor Cells/metabolism , Retinal Cone Photoreceptor Cells/pathology , Retinal Rod Photoreceptor Cells/metabolism , Retinal Rod Photoreceptor Cells/pathology , Retinitis Pigmentosa/genetics
2.
Gene Ther ; 17(9): 1162-74, 2010 Sep.
Article in English | MEDLINE | ID: mdl-20428215

ABSTRACT

A prerequisite for using corrective gene therapy to treat humans with inherited retinal degenerative diseases that primarily affect rods is to develop viral vectors that target specifically this population of photoreceptors. The delivery of a viral vector with photoreceptor tropism coupled with a rod-specific promoter is likely to be the safest and most efficient approach to target expression of the therapeutic gene to rods. Three promoters that included a fragment of the proximal mouse opsin promoter (mOP), the human G-protein-coupled receptor protein kinase 1 promoter (hGRK1), or the cytomegalovirus immediate early enhancer combined with the chicken ß actin proximal promoter CBA were evaluated for their specificity and robustness in driving GFP reporter gene expression in rods, when packaged in a recombinant adeno-associated viral vector of serotype 2/5 (AAV2/5), and delivered via subretinal injection to the normal canine retina. Photoreceptor-specific promoters (mOP, hGRK1) targeted robust GFP expression to rods, whereas the ubiquitously expressed CBA promoter led to transgene expression in the retinal pigment epithelium, rods, cones and rare Müller, horizontal and ganglion cells. Late onset inflammation was frequently observed both clinically and histologically with all three constructs when the highest viral titers were injected. Cone loss in the injected regions of the retinas that received the highest titers occurred with both the hGRK1 and CBA promoters. Efficient and specific rod transduction, together with preservation of retinal structure was achieved with both mOP and hGRK1 promoters when viral titers in the order of 10(11)vg ml(-1) were used.


Subject(s)
Dependovirus/genetics , Genetic Therapy/methods , Promoter Regions, Genetic , Retinal Rod Photoreceptor Cells/metabolism , Actins/genetics , Actins/metabolism , Animals , Dogs , G-Protein-Coupled Receptor Kinases/genetics , G-Protein-Coupled Receptor Kinases/metabolism , Genes, Reporter/genetics , Genetic Vectors/genetics , Humans , Mice , Transfection
3.
Gene Ther ; 15(14): 1049-55, 2008 Jul.
Article in English | MEDLINE | ID: mdl-18337838

ABSTRACT

Specific cone-directed therapy is of high priority in the treatment of human hereditary retinal diseases. However, not much information exists about the specific targeting of photoreceptor subclasses. Three versions of the human red cone opsin promoter (PR0.5, 3LCR-PR0.5 and PR2.1), and the human blue cone opsin promoter HB569, were evaluated for their specificity and robustness in targeting green fluorescent protein (GFP) gene expression to subclasses of cones in the canine retina when used in recombinant adeno-associated viral vectors of serotype 5. The vectors were administered by subretinal injection. The promoter PR2.1 led to most effective and specific expression of GFP in the long- and medium-wavelength-absorbing cones (L/M cones) of normal and diseased retinas. The PR0.5 promoter was not effective. Adding three copies of the 35-bp LCR in front of PR0.5 lead to weak GFP expression in L/M cones. The HB569 promoter was not specific, and GFP was expressed in a few L/M cones, some rods and the retinal pigment epithelium. These results suggest that L/M cones, the predominant class of cone photoreceptors in the retinas of dogs and most mammalian species can be successfully targeted using the human red cone opsin promoter.


Subject(s)
Genetic Therapy/methods , Promoter Regions, Genetic , Retinal Cone Photoreceptor Cells/metabolism , Rod Opsins/genetics , Targeted Gene Repair , Animals , Color Vision Defects/metabolism , Color Vision Defects/therapy , Dependovirus/genetics , Dogs , Gene Expression , Genetic Vectors/administration & dosage , Green Fluorescent Proteins/genetics , Humans , Injections , Models, Animal , Transduction, Genetic/methods , Transgenes
4.
Mol Microbiol ; 6(10): 1395-411, 1992 May.
Article in English | MEDLINE | ID: mdl-1322485

ABSTRACT

The 90-kilobase (kb) virulence plasmid of Salmonella typhimurium is responsible for invasion from the intestines to mesenteric lymph nodes and spleens of orally inoculated mice. We used Tn5 and aminoglycoside phosphotransferase (aph) gene insertion mutagenesis and deletion mutagenesis of a previously identified 14-kb virulence region to reduce this virulence region to 7.8kb. The 7.8-kb virulence region subcloned into a low copy-number vector conferred a wild-type level of splenic infection to virulence plasmid-cured S. typhimurium and conferred essentially a wild-type oral LD50. Insertion mutagenesis identified five loci essential for virulence, and DNA sequence analysis of the virulence region identified six open reading frames. Expected protein products were identified from four of the six genes, with three of the proteins identified as doublet bands in Escherichia coli minicells. Three of the five mutated genes were able to be complemented by clones containing only the corresponding wild-type gene. Only one of the five deduced amino acid sequences, that of the positive regulatory element, SpvR, possessed significant homology to other proteins. The codon usage for the virulence genes showed no codon bias, which is consistent with the low levels of expression observed for the corresponding proteins. Consensus promoters for several different sigma factors were identified upstream of several of the genes, whereas only consensus Rho-dependent termination sequences were observed between certain of the genes. The operon structure of this virulence region therefore appears to be complex. The construction of the cloned 7.8-kb virulence region and the determination of the DNA sequence will aid in the further genetic analysis of the five plasmid-encoded virulence genes of S. typhimurium.


Subject(s)
Bacterial Proteins/genetics , DNA, Bacterial/genetics , Salmonella typhimurium/genetics , Amino Acid Sequence , Animals , Base Sequence , Cloning, Molecular , Codon , Consensus Sequence , DNA Transposable Elements , Genetic Complementation Test , Kanamycin Kinase , Mice , Molecular Sequence Data , Mutagenesis, Insertional , Open Reading Frames , Phenotype , Phosphotransferases/genetics , Plasmids , Salmonella Infections, Animal/microbiology , Salmonella typhimurium/pathogenicity , Virulence
5.
Infect Immun ; 58(8): 2651-8, 1990 Aug.
Article in English | MEDLINE | ID: mdl-2164511

ABSTRACT

We have confirmed that the 28,000-molecular-weight (28K) protein encoded by the virA gene of the 90-kilobase Salmonella typhimurium virulence plasmid is a virulence factor. It was previously shown that a Tn5 insertion, vir-22::Tn5, located in the virulence plasmid greatly attenuated virulence for mice and inhibited the production of a 28K protein (P.A. Gulig and R. Curtiss III, Infect. Immun. 56:3262-3271, 1988). Plasmid pYA426 fully complemented vir-22::Tn5 to virulence by increasing splenic infection after oral inoculation and encoded the 28K protein. To identify the virulence gene(s) of pYA426 mutated by vir-22::Tn5, we constructed nested deletions in pYA426 and examined deletion derivatives for their abilities to complement vir-22::Tn5. Only derivatives still producing the 28K protein complemented vir-22::Tn5. Furthermore, the smallest complementing derivative encoded only the 28K protein, as determined by DNA sequence analysis. Therefore, the 28K protein is sufficient for complementation of the attenuating mutation vir-22::Tn5 and must be the virulence factor inhibited by the insertion. We determined the nucleotide sequence of the 1.2-kilobase BamHI-EcoRI fragment encoding the 28K protein and identified the structural gene, virA. A 723-base-pair open reading frame which encodes a peptide with a molecular weight of 27,572 was found.


Subject(s)
Bacterial Proteins/genetics , Genes, Bacterial , Plasmids/genetics , Salmonella typhimurium/pathogenicity , Virulence Factors , Amino Acid Sequence , Base Sequence , Cloning, Molecular , DNA Transposable Elements , DNA, Bacterial , Escherichia coli/genetics , Genetic Complementation Test , Molecular Sequence Data , Molecular Weight , Mutation , Salmonella typhimurium/genetics , Virulence/genetics
6.
J Immunol ; 139(1): 169-74, 1987 Jul 01.
Article in English | MEDLINE | ID: mdl-2953811

ABSTRACT

The effects of injecting urokinase into subdermal air sacs on the back of mice was studied. Urokinase was leukotactic in the concentration range of 2 X 10(-13) to 2 X 10(-15) M. This response was absolutely dependent on the enzyme activity of the serine esterase, but was found to be independent of generation of the chemotactic complement split product C5a. At high doses of urokinase (greater than 2 X 10(-12) M), no cellular infiltration was observed. Injection of 2 X 10(-10) M urokinase i.p. led to the systemic desensitization of mice when challenged in the skin with a lower dose (2 X 10(-14) M) of urokinase. Urokinase desensitization did not alter the ability of mice to respond to the chemical chemotactic factor f-met-leu-phe or to respond to C5a-dependent chemotactic stimuli. Urokinase desensitized mice failed to demonstrate a chemotactic response to nerve growth factor, thrombin, plasmin, or factor X activating enzyme, all of which were chemotactic in non-urokinase pre-treated animals. The results of these studies indicate the presence of three physiologically independent inflammatory pathways in mice: independent of C5 and not influenced by pretreatment with urokinase, independent of C5 and inhibited by pretreatment with urokinase, and dependent on C5 and not influenced by pretreatment with urokinase.


Subject(s)
Chemotaxis, Leukocyte , Metalloendopeptidases , Neutrophils/physiology , Urokinase-Type Plasminogen Activator/physiology , Animals , Dose-Response Relationship, Drug , Endopeptidases/metabolism , Female , Fibrinolysin/physiology , Inflammation/physiopathology , Mice , N-Formylmethionine Leucyl-Phenylalanine/pharmacology , Nerve Growth Factors/pharmacology , Staphylococcal Protein A/pharmacology , Thrombin/physiology
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