ABSTRACT
The effectiveness, both immunological (by an increase in the titers of antitoxins) and protective (by resistance to the inoculation of the absolute lethal dose of infective agents), of the regional (wound) revaccination with tetratoxoid (Clostridium perfringens, C. oedematiens, C. septicum, C. histolyticum) was demonstrated on the experimental model of wound infection (gas gangrene) of guinea pigs. The schedule of rapid immunization with tetratoxoid was developed, which made it possible to create good immunological preparedness (basic immunity) for subsequent revaccination in case of traumas within 6 days. The effectiveness of rapid immunization by the application of tetratoxoid on the wound was shown. This immunization ensured a considered increase in the titers of antitoxins within the first 6 days, which increased the protection of the animals from infection with each of the four causative agents of gas gangrene.
Subject(s)
Clostridium perfringens/immunology , Clostridium/immunology , Gas Gangrene/prevention & control , Toxoids/therapeutic use , Animals , Antitoxins/blood , Disease Models, Animal , Drug Evaluation, Preclinical , Emergencies , Gas Gangrene/immunology , Gas Gangrene/mortality , Guinea Pigs , Immunization, Secondary , Mice , Rabbits , Time Factors , Wound Infection/immunology , Wound Infection/mortality , Wound Infection/prevention & controlSubject(s)
Bacterial Vaccines/immunology , Clostridium perfringens/immunology , Gas Gangrene/immunology , Leukocytes, Mononuclear/immunology , Phagocytes/immunology , Toxoids/immunology , Wound Infection/immunology , Animals , Gas Gangrene/prevention & control , Guinea Pigs , Immunization, Secondary , Lymph Nodes/immunology , Wound Infection/prevention & controlABSTRACT
The study made with the use of complex methods established that the local (wound) application of tetanus toxoid rapidly made the manifestation of the lysosomal apparatus more pronounced, increased the oxidizing activity (determined in the nitro blue tetrazolium test) and phagocytic activity of the mononuclear phagocytizing system in the wound and in the regional lymph nodes. The wound application of tetanus toxoid significantly increased blast transformation of T lymphocytes in guinea pigs simultaneously with tetanus wound infection. The study confirmed the pathogenetic expediency of the proposed method for the stimulation of anti-tetanus immunity by the application of tetanus toxoid on the wound which specifically inhibited the primary stage of the infectious process.
