ABSTRACT
Melatonin plays an important role in cancer (tumor growth and metastasis) through different pathways and may have therapeutic significance.
Subject(s)
Adjuvants, Immunologic/pharmacology , Antioxidants/pharmacology , Cell Proliferation/drug effects , Gene Expression Regulation, Neoplastic/drug effects , Melatonin/metabolism , Melatonin/pharmacology , Neoplasms/metabolism , Adjuvants, Immunologic/metabolism , Antioxidants/metabolism , Apoptosis/drug effects , Cell Adhesion/drug effects , Cell Movement/drug effects , Humans , Neoplasm InvasivenessABSTRACT
The study of ENT cancer allows the implementation of molecular biology methods in diagnosis, predicting the evolution of the disease and suggesting a certain treatment. MMPs are proteolytic enzymes, zinc dependent endopeptidases, secreted by tissues and proinflammatory cells that play a role in the clearance of cell surface receptors. They are expressed as zymogens (inactive forms). Proteolytic enzymes cleave zymogens generating active forms. They are involved in cell proliferation, adhesion, differentiation, migration, angiogenesis, apoptosis and host defense.
Subject(s)
Carcinoma, Squamous Cell/enzymology , Extracellular Matrix/metabolism , Gene Expression Regulation, Enzymologic/physiology , Gene Expression Regulation, Neoplastic/physiology , Matrix Metalloproteinases/metabolism , Molecular Biology/methods , Otorhinolaryngologic Neoplasms/enzymology , Angiogenesis Inducing Agents/metabolism , Focal Adhesion Protein-Tyrosine Kinases/metabolism , Gene Expression Regulation, Enzymologic/genetics , Gene Expression Regulation, Neoplastic/genetics , Humans , Matrix Metalloproteinase Inhibitors/metabolism , Matrix Metalloproteinases/classificationABSTRACT
The observation of a 17-year-old teenager with progeria, ventricular septal shortcoming with wide anterolateral [correction of arteriolateral] myocardial ischemia. From an ocular point of view, he shows senescence with senile ectropion, evolutive cataract, retinal angiosclerosis. The role of the manifestation of ocular senescence in Hutchinson-Gilford Syndrome is emphasized.
Subject(s)
Cataract/diagnosis , Ectropion/diagnosis , Progeria/diagnosis , Retinal Vessels/pathology , Adolescent , Heart Septal Defects, Ventricular/diagnosis , Humans , Male , Myocardial Ischemia/diagnosis , Prostatic Hyperplasia/diagnosis , Retinal Diseases/diagnosis , Sclerosis/diagnosisSubject(s)
Endotoxins/pharmacology , Liver/drug effects , Salmonella typhi , Animals , In Vitro Techniques , Liver/ultrastructure , SwineABSTRACT
In view of applying an experimental method of transplantation of the liver (of the heterotopic type) within the possibilities of the team, and that could be applied in humans, experiments were performed in both pigs and dogs. Details of this procedure are presented, as well as the factors that could lead to a successful completion of the experiment. The results obtained by the authors have improved in parallel with the standardization of the technique and the skill of the team.
Subject(s)
Liver Transplantation , Animals , Dogs , Liver Circulation , Postoperative Complications , Swine , Transplantation, Heterologous , Vascular Surgical ProceduresABSTRACT
In view of providing hepatic assistance, and for conservation aims in attempts at transplantation conditions have been investigated for achieving extracorporeal normothermal perfusion of the liver isolated from pig and dog. A total of 33 experiments have been performed. The preparations and the devices used allow to apply the method in a patient only after stabilisation of the perfusion, and frees the medical team from a series of technical problems. The duration and the quality of the perfusion are especially influenced by the collecting technique. In the dog the liver should be removed 3--5 hours, and in the pig 7--8 hours before perfusion is started. The functional condition of the liver can be assessed at 45 minutes after the start of the perfusion by evaluating the following criteria: the macroscopic aspect of the liver, the blood flow, the biliary flow, the ascitis fluid, TGP and oxygen consumption. Adequate installations and a permanent involvement of the laboratory team are essential conditions for the application of this method to the treatment of acute severe hepatic failure.
