Características climáticas y epidemiológicas asociadas al cólico renal en una zona urbana en España / Climate and Epidemiological Characteristics of Renal Colic Attendances in an Urban Setting in Spain

Objetivos: Determinar la incidencia real de los episodios de cólico renal (CR) en nuestro ámbito, así como su relación con varios factores epidemiológicos, estacionales y climáticos. Material y Métodos: Hemos analizado los registros de 156.687 atenciones en el Servicio de Urgencias del Hospital Infanta Cristina (Parla, Madrid, España), desde su apertura el 7 de abril de 2008 hasta la fecha del análisis (28 de marzo de 2010). Se registraron la fecha de nacimiento, el sexo, la historia de episodios previos de CR, el motivo de consulta y la fecha de la atención. Se obtuvieron también los valores diarios de algunos parámetros climáticos (temperatura máxima y humedad relativa). Resultados: Se registraron un total de 1.866 episodios de CR (1,19% de todas las atenciones). La edad osciló entre los 15 y los 94 años, con una mediana de 39. Los episodios de CR fueron más prevalentes en la población masculina (58,4% vs. 41,6% en la femenina, respectivamente, p<0,001). No se observaron diferencias con respecto a la historia previa de CR. Se observó un modesto pero significativo aumento en la incidencia de CR durante las estaciones de verano y otoño. No existió correlación significativa entre el número de atenciones por CR y los parámetros climáticos estudiados. Conclusiones: La incidencia de CR en nuestro ambiente es similar a la existente en la literatura. Se registró un aumento modesto pero significativo de la incidencia durante el verano y otoño, aunque no se observó relación significativa con los valores climáticos de temperatura y humedad. La ausencia de cambios estacionales importantes en la incidencia de CR puede explicarse por las características meteorológicas “no-extremas” del ambiente estudiado (AU)

Objectives: To address the real incidence of RC episodes in our setting and its relationship with several epidemiological, seasonal and climatic factors. Material and Methods: We analyzed 156,687 attendances in the emergency unit of Hospital Infanta Cristina (Parla, Madrid, Spain), from the opening of the unit in 07/04/2008 to the date of analysis (28/03/2010). Date of birth, sex, history of previous urinary lithiasis episodes, main cause and date of attendance were collected. Daily climate parameters (maximum daily temperature and percent relative humidity) were recorded. Results: A total number of 1,866 RC episodes (1.19% of all attendances) were recorded during the study period. Age ranged from 15 to 94 years, median 39. RC episodes were more prevalent in male population (58.4% vs 41.6% in females respectively, p<0.001). No differences were observed with regard to previous history of RC. A modest but significant rise in RC incidence was observed during summer and autumn. No significant correlation was observed between monthly or seasonal number of RC attendances and the climatic parameters studied. Conclusions: RC incidence in our setting is similar to the previously reported in the literature. A modest but significant higher incidence of renal colic episodes were observed during summer and autumn seasons, although no significant relationship was attributed to temperature and humidity values. Absence of dramatic seasonal changes in incidence can be explained by the “non-extreme” weather conditions in the studied setting (AU)

[Climate and epidemiological characteristics of renal colic attendances in an urban setting in Spain]. / Características climáticas y epidemiológicas asociadas al cólico renal en una zona urbana en España.

OBJECTIVES: To address the real incidence of RC episodes in our setting and its relationship with several epidemiological, seasonal and climatic factors. MATERIAL AND METHODS: We analyzed 156,687 attendances in the emergency unit of Hospital Infanta Cristina (Parla, Madrid, Spain), from the opening of the unit in 07/04/2008 to the date of analysis (28/03/2010). Date of birth, sex, history of previous urinary lithiasis episodes, main cause and date of attendance were collected. Daily climate parameters (maximum daily temperature and percent relative humidity) were recorded. RESULTS: A total number of 1,866 RC episodes (1.19% of all attendances) were recorded during the study period. Age ranged from 15 to 94 years, median 39. RC episodes were more prevalent in male population (58.4% vs 41.6% in females respectively, p<0.001). No differences were observed with regard to previous history of RC. A modest but significant rise in RC incidence was observed during summer and autumn. No significant correlation was observed between monthly or seasonal number of RC attendances and the climatic parameters studied. CONCLUSIONS: RC incidence in our setting is similar to the previously reported in the literature. A modest but significant higher incidence of renal colic episodes were observed during summer and autumn seasons, although no significant relationship was attributed to temperature and humidity values. Absence of dramatic seasonal changes in incidence can be explained by the "non-extreme" weather conditions in the studied setting.

Inhibition of phospholipid methylation by a cytosolic factor.

Rat liver cytosol contains a heat-stable factor which inhibits phospholipid methylation by rat liver microsomes. The effect of this factor on lipid methylation was dose- and pH-dependent. This factor has an Mr of approx. 3200 as estimated by gel filtration. It could not be extracted by chloroform/methanol (2:1, v/v), and its action was inhibited by incubation with subtilisin.

Beta-adrenergic control of phosphatidylcholine synthesis by transmethylation in hepatocytes from juvenile, adult and adrenalectomized rats.

Changes in isoprenaline-sensitive phospholipid methyltransferase were studied in hepatocytes isolated from juvenile, mature and adrenalectomized rats. Isoprenaline produced greater stimulation of cyclic AMP accumulation in juvenile and mature adrenalectomized rats than in mature animals. Similarly, isoprenaline stimulated phospholipid methyltransferase in juvenile and mature adrenalectomized rats but had no effect in mature animals. Isoprenaline-mediated activation of phospholipid methyltransferase in adrenalectomized rats was time- and dose-dependent. In hepatocytes isolated from adrenalectomized rats incubated with [Me-3H]methionine or [3H]-ethanolamine the addition of isoprenaline increased the amount of radioactivity incorporated into phosphatidylcholine. The activation by isoprenaline of phospholipid methyltransferase was abolished by the beta-blocker propranolol and by insulin. These results indicate that rat liver the occupation of functional beta-receptors causes a stimulation of phospholipid methylation. It is suggested that, as reported previously, cyclic AMP activates phospholipid methyltransferase.

Stimulation by epidermal growth factor of phospholipid methyltransferase in isolated rat hepatocytes.

Epidermal growth factor produces a time- and dose-dependent activation of phospholipid methyltransferase activity in hepatocytes isolated from juvenile and mature hepatectomized rats. This treatment however has no effect with hepatocytes isolated from mature or laparotomized rats. Dansylcadaverine (50 microM), an inhibitor of receptor-mediated internalization of epidermal growth factor, has no effect on basal phospholipid methyltransferase but inhibits the stimulation of this enzyme by epidermal growth factor. These results indicate a possible role of phospholipid methylation during liver proliferation.

Phospholipid turnover during phagocytosis in human polymorphonuclear leucocytes.

We have previously observed that the phagocytosis of zymosan particles coated with complement by human polymorphonuclear leucocytes is accompanied by a time- and dose-dependent inhibition of phosphatidylcholine synthesis by transmethylation [García Gil, Alonso, Sánchez Crespo & Mato (1981) Biochem. Biophys. Res. Commun.101, 740-748]. The present studies show that phosphatidylcholine synthesis by a cholinephosphotransferase reaction is enhanced, up to 3-fold, during phagocytosis by polymorphonuclear cells. This effect was tested by both measuring the incorporation of radioactivity into phosphatidylcholine in cells labelled with [Me-(14)C]choline, and by assaying the activity of CDP-choline:diacylglycerol cholinephosphotransferase. The time course of CDP-choline:diacylglycerol cholinephosphotransferase activation by zymosan mirrors the inhibition of phospholipid methyltransferase activity previously reported. The extent of incorporation of radioactivity into phosphatidylcholine induced by various doses of zymosan correlates with the physiological response of the cells to this stimulus. This effect was specific for phosphatidylcholine, and phosphatidyl-ethanolamine turnover was not affected by zymosan. The purpose of this enhanced phosphatidylcholine synthesis is not to provide phospholipid molecules rich in arachidonic acid. The present studies show that about 80% of the arachidonic acid generated in response to zymosan derives from phosphatidylinositol. A transient accumulation of arachidonoyldiacylglycerol has also been observed, which indicates that a phospholipase C is responsible, at least in part, for the generation of arachidonic acid. Finally, isobutylmethylxanthine and quinacrine, inhibitors of phosphatidylinositol turnover, inhibit both arachidonic acid generation and phagocytosis, indicating a function for this pathway during this process.