1.
Curr Protoc Immunol
; Chapter 6: 6.16.1-6.16.15, 2001 May.
Article
in English
| MEDLINE
| ID: mdl-18432807
ABSTRACT
This unit describes functional assays for measurement of bioactive IL-12 and ELISAs for measurement of IL-12 protein. The functional assays are based on the ability of IL-12 to stimulate proliferation of PHA-activated T lymphoblasts ("PHA blasts"). The ELISAs are technically simpler to perform than the functional assays, but cannot distinguish bioactive from inactive cytokine.
