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Radiat Environ Biophys ; 59(4): 733-741, 2020 11.
Article in English | MEDLINE | ID: mdl-32914274

ABSTRACT

While radiation-induced lung injury (RILI) is known to be progressed by Th2 skewed, pro-inflammatory immune response, there have been few therapeutic attempts through Th1 immune modulation. We investigated whether the immunostimulant CpG-oligodeoxynucleotide (CpG-ODN) would be effective against RILI by way of measuring reactive oxygen species (ROS) and nitric oxides (NO), histopathology, micro-three-dimensional computer tomography (CT), and cytokine profiling. We found that KSK CpG-ODN (K-CpG) significantly reduced histopathological fibrosis when compared to the positive control (PC) group (p < 0.01). The levels of ROS production in serum and splenocyte of PC group were significantly higher than that of K-CpG group (p < 0.01). The production of nitric oxide (NO) in CpG-ODNs group was higher than that of PC group. Last, cytokine profiling illustrated that the protein concentrations of Th1-type cytokines such as IL-12 and TNF-α as well as Th2-type cytokine IL-5 in K-CpG group inclined to be significantly (p < 0.001 or p < 0.01) higher than those of in PC group. Collectively, our study clearly indicates that K-CpG is effective against RILI in mice by modulating the innate immune response. To our knowledge, this is the first note on anti-RILI effect of human type, K-CpG, clinically implying the potential of immunotherapy for RILI control.


Subject(s)
Lung Injury/drug therapy , Oligodeoxyribonucleotides/therapeutic use , Radiation Injuries, Experimental/drug therapy , Animals , Cytokines/blood , Female , Lung/diagnostic imaging , Lung/drug effects , Lung/immunology , Lung/pathology , Lung Injury/diagnostic imaging , Lung Injury/immunology , Lung Injury/pathology , Mice, Inbred C57BL , Nitric Oxide/immunology , Oligodeoxyribonucleotides/pharmacology , Radiation Injuries, Experimental/diagnostic imaging , Radiation Injuries, Experimental/immunology , Radiation Injuries, Experimental/pathology , Reactive Oxygen Species/immunology , Spleen/cytology , Spleen/drug effects , Spleen/radiation effects , Tomography, X-Ray Computed , X-Rays
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