Detection of Micrometer-Sized Virus Aerosols by Using a Real-Time Bioaerosol Monitoring System.

This study investigates a real-time handheld bioaerosol monitoring system for the detection of biological particles using UV-LED and light-induced fluorescence technology. Biological particles produce both scattering and fluorescence signals simultaneously, which can help distinguish them from general particles. The detected scattering, fluorescence, and simultaneous signals are then converted into photon signals and categorized based on predetermined criteria. A reliable biological particle generator was required to validate the performance of the system. This study explores the use of an M13 bacteriophage as a virus simulant of biological agents and employs a customized inkjet aerosol generator to produce M13 bacteriophage aerosols of a specific size by controlling the concentration of M13. We confirmed that micro-sized, narrowly dispersed M13 aerosols were efficiently generated. Additionally, we confirmed the performance of this real-time handheld bioaerosol monitoring system by detecting viruses.

Comparative Evaluation of Flexural Toughness of Steel Fiber-Reinforced Concrete Beams.

Specifications are available to quantify flexural performance of steel-fiber reinforced concrete beams with several parameters. Each specification provides different results. This study comparatively evaluates existing flexural beam test standards that are used to evaluate the flexural toughness of SFRC beam specimens. Two standards, EN-14651 and ASTM C1609, were followed to test SFRC beams under the three-point bending test (3PBT) and the four-point bending test (4PBT), respectively. Both normal tensile strength steel fiber (1200 MPa) and high tensile strength steel fiber (1500 MPa) in high-strength concrete were considered in this study. The reference parameters recommended in the two standards, which include equivalent flexural strength, residual strength, energy absorption capacity, and flexural toughness, were compared based on the tensile strength (normal or high) of the steel fiber in high-strength concrete. The 3PBT and 4PBT results indicate that both standard test methods yield similar results to quantify the flexural performance of SFRC specimens. However, unintended failure modes were observed for both standard test methods. The adopted correlation model shows that the flexural performance of SFRC is similar for 3PBTs and 4PBTs, but the residual strength obtained from the 3PBTs tends to be greater than that obtained from 4PBTs with an increase in the tensile strength of steel fiber.

Optimizing the Sensitivity of Biological Particle Detectors through Atmospheric Particle Analysis According to Climatic Characteristics in South Korea.

Biological agents used in biological warfare or bioterrorism are also present in bioaerosols. Prompt identification of a biological weapon and its characteristics is necessary. Herein, we optimized an environmentally adaptive detection algorithm that can better reflect changes in the complex South Korean environment than the current models. The algorithm distinguished between normal and biological particles using a laser-induced fluorescence-based biological particle detector capable of real-time measurements and size classification. We ensured that the algorithm operated with minimal false alarms in any environment by training based on experimental data acquired from an area where rainfall, snow, fog and mist, Asian dust, and water waves on the beach occur. To prevent time and money wastage due to false alarms, the detection performance for each level of sensitivity was examined to enable the selection of multiple sensitivities according to the background, and the appropriate level of sensitivity for the climate was determined. The basic sensitivity was set more conservatively than before, with a 3% alarm rate at 20 agent-containing particles per liter of air (ACPLA) and a 100% alarm rate at 63 ACPLA. The reliability was increased by optimizing five variables. False alarms did not occur in situations where no alarm was unnecessary.

Internal Relative Humidity, Autogenous Shrinkage, and Strength of Cement Mortar Modified with Superabsorbent Polymers.

Laboratory evaluations were performed to investigate the effect of internal curing (IC) by superabsorbent polymers (SAP) on the internal relative humidity (IRH), autogenous shrinkage, coefficient of thermal expansion (CTE), and strength characteristics of low water-cement ratio (w/c) mortars. Four types of SAP with different cross-linking densities and particle sizes were used. Test results showed that the SAP inclusion effectively mitigated the IRH drops due to self-desiccation and corresponding autogenous shrinkage, and the IC effectiveness tended to increase with an increased SAP dosage. The greater the cross-linking density and particle size of SAP, the less the IRH drop and autogenous shrinkage. The trend of autogenous shrinkage developments was in good agreement with that of IRH changes, with nearly linear relationships between them. Both immediate deformation (ID)-based and full response-based CTEs were rarely affected by SAP inclusions. There were no substantial losses in compressive and flexural strengths of SAP-modified mortar compared to reference plain mortar. The findings revealed that SAPs can be effectively used to reduce the shrinkage cracking potential of low w/c cement-based materials at early ages, without compromising mechanical and thermal characteristics.