ABSTRACT
The mean count of cells with chromosome aberrations increased in a 72-h culture of peripheral blood lymphocytes of children with Helicobacter pylori-associated gastroduodenal diseases. After eradication therapy, intensification of clastogenesis was observed in the majority of children. Addition of vetoron to the treatment protocols reduced manifestations of clastogenesis.
Subject(s)
Chromosome Aberrations/statistics & numerical data , Helicobacter Infections/genetics , Adolescent , Child , Child, Preschool , Chromosome Disorders/prevention & control , Duodenitis/complications , Female , Gastritis/complications , Helicobacter Infections/blood , Helicobacter Infections/drug therapy , Helicobacter pylori , Humans , Male , beta Carotene/therapeutic useABSTRACT
Five workers of molybdenum gilding at the metallurgical works were observed prior to and after administration of ascorbic acid. Tests for chromosomal aberrations (CA) and sister chromatid exchanges (SCE) were used as the criteria for estimation. Individual sensitivity to the chemicals was revealed in the workers. Administration of ascorbic acid caused reduction of the level of chromosomal structural rearrangements.
Subject(s)
Ascorbic Acid/therapeutic use , Chromosome Aberrations , Lymphocytes/drug effects , Molybdenum/toxicity , Occupational Exposure , Sister Chromatid Exchange , Cells, Cultured , Humans , Molybdenum/chemistry , SaltsABSTRACT
As a result of research it is established that molybdenum shows the most pronounced genetic activity at the early stages of spermatogenesis. Genetic effects of molybdenum and its modification by vitamin S are registered in the test of dominant lethal mutations. The cytogenetic activity of molybdenum, the molybdenic candle-end and its modification by antibacterial preparation have been revealed in the cells of mammalian marrow.
Subject(s)
Ascorbic Acid/pharmacology , Drosophila melanogaster/genetics , Molybdenum/toxicity , Mutagens/toxicity , Quinoxalines/toxicity , Spermatogenesis/genetics , Animals , Drosophila melanogaster/drug effects , Male , Mice , Spermatogenesis/drug effectsABSTRACT
Ammonium molybdate cadmium iodide and cadmium chloride have been studied in test for their genotoxic effect on induction of DNA-cellular bonding, extrasynthesis of DNA in spermatozoa of mice as well as in test to estimate a fertility criterion of Drosophila males. Ammonium molybdate, cadmium iodide and cadmium chloride are stated to be able to induce injuries of native DNA in test on induction of DNA-cellular bonding and DNA-sex cells of mice and Drosophila melanogaster in dominant-lethal test and in experiments on estimation of a fertility coefficient of Drosophila males, respectively.
Subject(s)
Cadmium/toxicity , Genes/drug effects , Molybdenum/toxicity , Animals , Cadmium Chloride , DNA/biosynthesis , DNA/drug effects , Drosophila melanogaster , Fertility/drug effects , Fertility/genetics , Male , Mice , Mice, Inbred C57BL , Mice, Inbred CBA , Spermatozoa/drug effects , Spermatozoa/metabolismABSTRACT
Heavy metal salts, the workers from molybdenum, tungsten and cobalt plants to make in contact with, reveal their mutagenic activity. Individual sensitivity to heavy metal salts has been analyzed through the example of molybdenum. Chromosome aberrations and sister chromatid exchanges have been studied for the regularities of their formation in lymphocytes of workers depending on the length of service. Sensitivity of tests of chromosome aberrations and sister chromatid exchanges has been compared to reveal genetic consequences of these types of the effects.
Subject(s)
Chromosome Aberrations , Metals/adverse effects , Occupational Exposure/adverse effects , Sister Chromatid Exchange/drug effects , Humans , Lymphocytes/drug effects , Lymphocytes/ultrastructure , Metallurgy , Mutagenicity Tests/methods , Occupational Exposure/statistics & numerical data , Risk Factors , USSRSubject(s)
Ascorbic Acid/pharmacology , Metallurgy , Metals/adverse effects , Mutation/drug effects , Occupational Exposure/adverse effects , Cells, Cultured/drug effects , Cells, Cultured/ultrastructure , Chromosome Aberrations , DNA/drug effects , DNA Damage/drug effects , Humans , Lymphocytes/drug effects , Lymphocytes/ultrastructure , Sister Chromatid Exchange/drug effectsSubject(s)
Ascorbic Acid/therapeutic use , Cadmium/adverse effects , DNA Damage , DNA Repair/drug effects , DNA, Single-Stranded/drug effects , DNA/drug effects , Mutation/drug effects , Occupational Exposure/adverse effects , Vitamin A/therapeutic use , 4-Nitroquinoline-1-oxide/pharmacology , Cadmium Chloride , Cells, Cultured/drug effects , Cells, Cultured/metabolism , Cells, Cultured/radiation effects , DNA/biosynthesis , DNA/radiation effects , DNA Repair/radiation effects , DNA, Single-Stranded/biosynthesis , DNA, Single-Stranded/radiation effects , Drug Evaluation, Preclinical , Humans , Lymphocytes/drug effects , Lymphocytes/metabolism , Lymphocytes/radiation effects , Mutation/radiation effects , Stimulation, Chemical , Ultraviolet RaysABSTRACT
The workers contacting with cobalt chloride for 10 years and more have been receiving 50 mg ascorbic acid for 30 days. DNA repair was analysed after this procedure in lymphocytes cultivated in vitro according to following criteria: reactivation and induced mutagenesis of vaccinia virus as well as formation of DNA breaks and their resynthesis upon treatment with cobalt chloride and 4-nitro-quinoline-1-oxide.
Subject(s)
Ascorbic Acid/pharmacology , Cobalt/toxicity , Mutagens , Occupational Exposure , 4-Nitroquinoline-1-oxide/toxicity , Cells, Cultured , Cobalt/antagonists & inhibitors , DNA Damage , DNA Repair/drug effects , Humans , Lymphocytes/drug effects , Lymphocytes/microbiology , Vaccinia virus/geneticsABSTRACT
Mechanisms of DNA repair in lymphocytes of persons having occupational contact with heavy metal salts for at least 10 years were investigated. The long-term exposure to heavy metal salts did not result in development of mechanisms of adaptation to the respective metals, as shown by increased DNA sensitivity to these compounds and by decrease in the repair activity. However, in some cases, resistance to other mutagens, 4-NQO, in particular, was observed.
Subject(s)
Cobalt/toxicity , DNA Damage , DNA Repair , Environmental Exposure , Molybdenum/toxicity , Mutagens , Humans , Lymphocytes/drug effects , Lymphocytes/metabolismABSTRACT
Analysis of data on the influence of different metals (cadmium, cobalt, molybdenum) on the Drosophila melanogaster cells has revealed a considerable genotoxic effect of cadmium salts which manifests in the high percentage of cells elimination at the early stages of spermatogenesis--the premeiotic cells. The modifying effect of molybdenum in the combination with cadmium and molybdenum decreasing the percentage of the progeny fall may be induced by activation of the reparation systems. In the bone marrow cells of mice Cd ions modify cytogenetic effects of the AB preparation, intensifying its mutagenic activity in some cases.
