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Int Arch Allergy Immunol ; 138(2): 134-41, 2005 Oct.
Article in English | MEDLINE | ID: mdl-16179794

ABSTRACT

BACKGROUND: Rhodotorula mucilaginosa is one of the most frequently encountered species of yeasts in our environment. We reported here a major allergen of R. mucilaginosa. METHODS: A major R. mucilaginosa allergen (Rho m 2) was characterized by two-dimensional (2D) immunoblotting, protein sequencing, cDNA cloning and IgE cross-reactivity with fungal serine proteases. RESULTS: Fourty-four sera (28%) from 157 bronchial asthmatic patients showed IgE-immunoblot reactivity against R. mucilaginosa extract. Among these 44 sera, 25 (57%) demonstrated IgE binding against a 31-kDa protein of R. mucilaginosa. Protein sequencing results suggest that it is a vacuolar serine protease. The corresponding cDNA clone encoding a mature protein of 312 residues was isolated. It shares 67-68% sequence identity with vacuolar serine protease allergens from three different Penicillium species (Pen ch 18, Pen o 18 and Pen c 18) and designated as Rho m 2 by the Allergen Nomenclature Committee. The native and recombinant Rho m 2 react with IgE antibodies and monoclonal antibody (MoAb) FUM20 against fungal serine proteases. IgE cross-reactivity between nRho m 2 and nPen ch 18 was observed. It was also detectable between rRho m 2 and rPen o 18. CONCLUSION: Our results suggest that R. mucilaginosa may also be a significant causative agent of human respiratory allergic disorders. We identified a vacuolar serine protease as a major allergen of R. mucilaginosa (Rho m 2) and a pan allergen of prevalent airborne fungal species. We detected IgE cross-reactivity among these highly conserved serine protease pan-fungal allergens.


Subject(s)
Allergens/immunology , Antigens, Fungal/immunology , Rhodotorula/immunology , Serine Endopeptidases/immunology , Allergens/classification , Allergens/genetics , Amino Acid Sequence , Antigens, Fungal/classification , Antigens, Fungal/genetics , Base Sequence , Conserved Sequence , Cross Reactions/immunology , DNA, Complementary/genetics , Humans , Immunoglobulin E/immunology , Molecular Sequence Data , Recombinant Proteins/biosynthesis , Recombinant Proteins/genetics , Recombinant Proteins/immunology , Rhodotorula/enzymology , Rhodotorula/genetics , Serine Endopeptidases/genetics , Vacuoles/enzymology
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